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Surfaces modified with nanometer-thick silver-impregnated polymeric films that kill bacteria but support growth of mammalian cells
Open accessAbstract
Silver is widely used as a biocidal agent in ointments and wound dressings. However, it has also been associated with tissue toxicity and impaired healing. In vitro characterization has also revealed that typical loadings of silver employed in ointments and dressings (∼ 100 μg/cm2) lead to cytotoxicity. In this paper, we report the results of an initial study that sought to determine if localization of carefully controlled loadings of silver nanoparticles within molecularly thin films immobilized on surfaces can lead to antimicrobial activity without inducing cytotoxicity. Polymeric thin films of poly(allylamine hydrochloride) (PAH) and poly(acrylic acid) (PAA) were prepared by layer-by-layer deposition and loaded with ∼0.4 μg/cm2 to ∼23.6 μg/cm2 of silver nanoparticles. Bacterial killing efficiencies of the silver-loaded films were investigated against Staphylococcus epidermidis, a gram-positive bacterium, and it was determined that as little as ∼0.4 μg/cm2 of silver in the polymeric films caused a reduction of 6 log10 CFU/mL (99.9999%) bacteria in suspensions incubated in contact with the films (water-borne assays). Significantly, whereas the antibacterial films containing high loadings of silver were found to be toxic to a murine fibroblast cell line (NIH-3T3), the polymeric films containing ∼0.4 μg/cm2 of silver were not toxic and allowed attachment, and growth of the mammalian cells. Thus, the results of this study go beyond prior reports by identifying silver-impregnated, polymeric thin films that are compatible with in vitro mammalian cell culture yet exhibit antibacterial activity. These results support the hypothesis that localization of carefully controlled loadings of silver nanoparticles within molecularly thin polymeric films can lead to antimicrobial activity without cytotoxicity. More broadly, this strategy of modifying surfaces with minimal loadings of bioactive molecules indicates the basis of approaches that may permit management of microbial burden in wound beds without impairment of wound healing.
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Cites
Two-level antibacterial coating with both release-killing and contact-killing capabilities
Li, Z., Lee, D., Sheng, X., Cohen, R. E., & Rubner, M. F. (2006) · Langmuir · doi:10.1021/la0622166 · Cited by 436 · cited 2×
“The first experiments performed in our study sought to characterize the interactions of mammalian cells with silver-containing PEMs of PAH/PAA that were prepared under conditions reported previously to lead to antimicrobial activity [25,26].”
Cited by
Banerjee, I., Pangule, R. C., & Kane, R. S. (2011) · Advanced Materials · doi:10.1002/adma.201001215 · Cited by 2,749
Silver as Antibacterial Agent: Ion, Nanoparticle, and Metal
Chernousova, S., & Epple, M. (2013) · Angewandte Chemie International Edition · doi:10.1002/anie.201205923 · Cited by 2,264
Antibacterial nano-structured titania coating incorporated with silver nanoparticles
Zhao, L., Wang, H., Huo, K., Cui, L., Zhang, W., Ni, H., Zhang, Y., Wu, Z., & Chu, P. K. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.04.040 · Cited by 772
Raphel, J., Holodniy, M., Goodman, S. B., & Heilshorn, S. C. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.01.016 · Cited by 674
Hu, H., Zhang, W., Qiao, Y., Jiang, X., Liu, X., & Ding, C. (2012) · Acta Biomaterialia · doi:10.1016/j.actbio.2011.09.031 · Cited by 484
Metadata
- Authors
- Agarwal, A., Weis, T. L., Schurr, M. J., Faith, N. G., Czuprynski, C. J., McAnulty, J. F., Murphy, C. J., & Abbott, N. L.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2010-02
- Volume
- 31
- Issue
- 4
- Pages
- 680-690
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W2024928939
- Cited by
- 251
- References
- 1
- FWCI
- 8.94
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Agarwal, A., Weis, T. L., Schurr, M. J., Faith, N. G., Czuprynski, C. J., McAnulty, J. F., Murphy, C. J., & Abbott, N. L. (2010). Surfaces modified with nanometer-thick silver-impregnated polymeric films that kill bacteria but support growth of mammalian cells. Biomaterials. https://doi.org/10.1016/j.biomaterials.2009.09.092
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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