Microphysiological head and neck cancer model identifies novel role of lymphatically secreted monocyte migration inhibitory factor in cancer cell migration and metabolism
Open accessAbstract
Regional metastasis of head and neck cancer (HNC) is prevalent (approximately 50% of patients at diagnosis), yet the underlying drivers and mechanisms of lymphatic spread remain unclear. The complex tumor microenvironment (TME) of HNC plays a crucial role in disease maintenance and progression; however, the contribution of the lymphatics remains underexplored. We created a primary patient cell derived microphysiological system that incorporates cancer-associated-fibroblasts from patients with HNC alongside a HNC tumor spheroid and a lymphatic microvessel to create an in vitro TME platform to investigate metastasis. Screening of soluble factor signaling identified novel secretion of macrophage migration inhibitory factor (MIF) by lymphatic endothelial cells conditioned in the TME. Importantly, we also observed patient-to-patient heterogeneity in cancer cell migration similar to the heterogeneity observed in clinical disease. Optical metabolic imaging at the single cell level identified a distinct metabolic profile of migratory versus non-migratory HNC cells in a microenvironment dependent manner. Additionally, we report a unique role of MIF in increasing HNC reliance on glycolysis over oxidative phosphorylation. This multicellular, microfluidic platform expands the tools available to explore HNC biology in vitro through multiple orthogonal outputs and establishes a system with enough resolution to visualize and quantify patient-to-patient heterogeneity.
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The presence of cancer-associated fibroblasts promotes cancer-cell migration in lymphatic vessel devices.
“In a later study, the same group showed that the presence of CAFs promotes cancer-cell migration in these devices (Figure 4F), but the role of lymphatic endothelial cells (LECs) herein remains to be investigated [121,122].”
From Cancer-on-chip models for metastasis: importance of the tumor microenvironment (2024) · cites this paper as [122]
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Cancer-on-chip models for metastasis: importance of the tumor microenvironment
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- Authors
- Yada, R. C., Desa, D. E., Gillette, A. A., Bartels, E., Harari, P. M., Skala, M. C., Beebe, D. J., & Kerr, S. C.
- Affiliations
- University of Wisconsin–Madison (US)
- Corresponding author
- Beebe, David J.
- Kerr, Sheena C.
- ORCID
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2023-07
- Volume
- 298
- Pages
- 122136
- ISSN
- 0142-9612 (ISSN-L)
- PMC
- PMC10205684
- OpenAlex
- W4367187079
- Cited by
- 30
- References
- 61
- Citations by year
- 2026: 10 · 2025: 9 · 2024: 8 · 2023: 3
- FWCI
- 3.27
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (am, 315 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Biomaterials (acceptedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (P30CA014520, T32 GM140935)
- Retina Research Foundation
- University of Wisconsin-Madison
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Macrophage Migration Inhibitory Factor
- Health Sciences › Medicine › Oncology › Lymphatic System and Diseases
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- head and neck cancer, lymph node metastasis, macrophage migration inhibitory factor, tumor microenvironment, lymphatic endothelial cells, tumor cell migration, cancer metabolism, glycolysis, oxidative phosphorylation, cancer-associated fibroblasts, tumor spheroids, optical metabolic imaging
- MeSH terms
- Cell Movement, Head and Neck Neoplasms, Humans, Macrophage Migration-Inhibitory Factors, Monocytes, Endothelial Cells, Cell Line, Tumor, Tumor Microenvironment
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