Gene Expression Signature in Peripheral Blood Cells from Medical Students Exposed to Chronic Psychological Stress
Abstract
To assess response to chronic psychological stress, gene expression profiles in peripheral blood from 18 medical students confronting license examination were analyzed using an original microarray. Total RNA was collected from each subject 9 months before the examination and mixed to be used as a universal control. At that time, most students had normal scores on the state-trait anxiety inventory (STAI). However, STAI scores were significantly elevated at 2 months and at 2 days before the examination. Pattern of the gene expression profile was more uniform 2 days before than 2 months before the examination. We identified 24 genes that significantly and uniformly changed from the universal control 2 days before the examination. Of the 24 genes, real-time PCR validated changes in mRNA levels of 10 (PLCB2, CSF3R, ARHGEF1, DPYD, CTNNB1, PPP3CA, POLM, IRF3, TP53, and CCNI). The identified genes may be useful to assess chronic psychological stress response.
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Stress resilience, the ability to manage anxiety under stress, is influenced by genetic factors.
“Stress resilience, the ability to manage anxiety under stress, is influenced by genetic factors.”
From Comprehensive review of chronic stress pathways and the efficacy of behavioral stress reduction programs (BSRPs) in managing diseases. (2024) · cites this paper as [17]
Upregulation of DRD1-DRD4, DBH, DAT, and BDNF is associated with higher resilience.
“Variants in genes like NPY, CRHR1, and 5-HTT are linked to lower resilience, while upregulation of DRD1–DRD4, DBH, DAT, and BDNF is associated with higher resilience.”
From Comprehensive review of chronic stress pathways and the efficacy of behavioral stress reduction programs (BSRPs) in managing diseases. (2024) · cites this paper as [17]
Studies on law students found that genetic variability in the NPS/NPSR1 system becomes more evident under chronic stress conditions like exam preparation.
“Similarly, studies on law students found that genetic variability in the NPS/NPSR1 system becomes more evident under chronic stress conditions like exam preparation [17,22,41,109].”
From Comprehensive review of chronic stress pathways and the efficacy of behavioral stress reduction programs (BSRPs) in managing diseases. (2024) · cites this paper as [17]
Variants in genes like NPY, CRHR1, and 5-HTT are linked to lower resilience.
“Variants in genes like NPY, CRHR1, and 5-HTT are linked to lower resilience, while upregulation of DRD1–DRD4, DBH, DAT, and BDNF is associated with higher resilience.”
From Comprehensive review of chronic stress pathways and the efficacy of behavioral stress reduction programs (BSRPs) in managing diseases. (2024) · cites this paper as [17]
Research on medical students facing license examinations revealed significant changes in gene expression profiles in peripheral blood, validating changes in 10 key genes (DPYD, CSF3R, PLCB2, CTNNB1, PPP3CA, IRF3, POLM, CCNI, ARHGEF1, and TP53).
“Research on medical students facing license examinations revealed significant changes in gene expression profiles in peripheral blood, validating changes in 10 key genes (DPYD, CSF3R, PLCB2, CTNNB1, PPP3CA, IRF3, POLM, CCNI, ARHGEF1, and TP53).”
From Comprehensive review of chronic stress pathways and the efficacy of behavioral stress reduction programs (BSRPs) in managing diseases. (2024) · cites this paper as [17]
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References 29 · Cited by 36
- Authors
- Kawai, T., Morita, K., Masuda, K., Nishida, K., Shikishima, M., Ohta, M., Saito, T., & Rokutan, K.
- Affiliations
- Tokushima University (JP)
- Hitachi (Japan) (JP)
- Corresponding author
- Rokutan, Kazuhito
- Venue
- Biological Psychology
- Publisher
- Elsevier BV
- Published
- 2007-10
- Volume
- 76
- Issue
- 3
- Pages
- 147-155
- ISSN
- 0301-0511 (ISSN-L)
- OpenAlex
- W2065037028
- Cited by
- 36
- References
- 29
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 2 · 2017: 1 · 2016: 1 · 2015: 2 · 2013: 7 · 2012: 3
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biological Psychology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Research Institute of Science and Technology for Society
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Science and Technology Agency
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
- Health Sciences › Medicine › Psychiatry and Mental health › Cancer, Stress, Anesthesia, and Immune Response
- Keywords
- chronic psychological stress, gene expression signatures, peripheral blood cells, medical students, state-trait anxiety, PLCβ2, CSF3R, ARHGEF1, dihydropyrimidine dehydrogenase, CTNNB1, PPP3CA, IRF3, TP53, mRNA expression
- MeSH terms
- Adaptation, Psychological, Adult, Anxiety, Blood Cells, Female, Gene Expression Regulation, Humans, Male, Personality Inventory, Stress, Psychological, Students, Medical, Cluster Analysis, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling
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