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Microalgae-associated bacteria play a key role in the flocculation of Chlorella vulgaris
Abstract
Flocculation is most preferred method for harvesting microalgae, however, the role of bacteria in microalgal flocculation process is still unknown. This study proves that bacteria play a profound role in flocculating by increasing the floc size resulting in sedimentation of microalgae. A flocculating activity of 94% was achieved with xenic Chlorella vulgaris culture as compared to 2% achieved with axenic culture. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene of xenic C. vulgaris culture revealed the presence of Flavobacterium sp., Terrimonas sp., Sphingobacterium sp., Rhizobium sp. and Hyphomonas sp. as microalgae-associated bacteria. However when Flavobacterium, Terrimonas, Sphingobacterium were eliminated by fluorescence activated cell sorter (FACS), flocculating activity reduced to 3%. Further studies with cell free extracts also suggest that bacterial extracellular substances might also have a role in enhancing flocculation. We conclude that the collective presence of certain bacteria is the determining factor in flocculation of C. vulgaris.
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“The flocculating activity reached up to 94% with the co-cultures, compared to just 2% achieved with the axenic culture.”
From Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia (2021) · cites this paper as [126]
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Metadata
- Authors
- Lee, J., Cho, D.-H., Ramanan, R., Kim, B.-H., Oh, H.-M., & Kim, H.-S.
- Affiliations
- Korea Research Institute of Bioscience and Biotechnology (KR)
- Corresponding author
- Kim, Hee-Sik
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2013-03
- Volume
- 131
- Pages
- 195-201
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2069771863
- Cited by
- 238
- References
- 39
- Citations by year
- 2026: 8 · 2025: 13 · 2024: 19 · 2023: 21 · 2022: 20 · 2021: 27 · 2020: 29 · 2019: 21 · 2018: 16 · 2017: 17 · 2016: 9 · 2015: 18 · 2014: 17 · 2013: 3
- FWCI
- 4.67
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- KRIBB Repository (submittedVersion)
- Funders
- Korea Research Institute of Bioscience and Biotechnology
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Physical Sciences › Environmental Science › Water Science and Technology › Coagulation and Flocculation Studies
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Keywords
- Chlorella vulgaris, microalgae harvesting, floc size, sedimentation, Flavobacterium, Terrimonas, Sphingobacterium, Rhizobium, Hyphomonas, xenic culture, axenic culture
- MeSH terms
- Flocculation, Bioreactors, Chlorella vulgaris, Microalgae
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