Extraction of intracellular protein from Chlorella pyrenoidosa using a combination of ethanol soaking, enzyme digest, ultrasonication and homogenization techniques
Abstract
Due to the rigid cell wall of Chlorella species, it is still challenging to effectively extract significant amounts of protein. Mass methods were used for the extraction of intracellular protein from microalgae with biological, mechanical and chemical approaches. In this study, based on comparison of different extraction methods, a new protocol was established to maximize extract amounts of protein, which was involved in ethanol soaking, enzyme digest, ultrasonication and homogenization techniques. Under the optimized conditions, 72.4% of protein was extracted from the microalgae Chlorella pyrenoidosa, which should contribute to the research and development of Chlorella protein in functional food and medicine.
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- Authors
- Zhang, R., Chen, J., & Zhang, X.
- Affiliations
- South China University of Technology (CN)
- Corresponding author
- Zhang, Ruilin
- Zhang, Xuewu
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2018-01
- Volume
- 247
- Pages
- 267-272
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2756153152
- Cited by
- 63
- References
- 14
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 10 · 2023: 9 · 2022: 7 · 2021: 6 · 2020: 7 · 2019: 6 · 2018: 3
- FWCI
- 1.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ocean and Fisheries Bureau of Guangdong Province (A201601C10)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Keywords
- Chlorella pyrenoidosa, microalgae, ethanol soaking, enzymatic digestion, sonication, homogenization, cell wall, extraction protocol optimization, microalgal proteins
- MeSH terms
- Ethanol, Chlorella, Proteins, Microalgae
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