Enhancing the longevity of microparticle-based glucose sensors towards 1 month continuous operation
Open accessAbstract
Luminescent microspheres encapsulating glucose oxidase have recently been reported as potential implantable sensors, but the operational lifetime of these systems has been limited by enzyme degradation. We report here that the longevity of these enzymatic microparticle-based sensors has been extended by the coimmobilization of glucose oxidase (GOx) and catalase (CAT) into the sensor matrix. A mathematical model was used to compare the response and longevity of the sensors with and without catalase. To experimentally test the longevity, sensors were continuously operated under normoglycemic dermal substrate concentrations and physiological conditions (5.5 mM glucose and 140 microM O(2), 37 degrees C and pH 7.4). The sensors incorporating CAT were experimentally shown to be approximately 5 times more stable than those without CAT; nevertheless, the response of sensors with CAT still changed by approximately 20%, when operated continuously for 7 days. The experimentally determined trends obtained for the variation in sensor response due to enzyme deactivation were in close agreement with modeling predictions, which also revealed a significant apparent loss in enzyme activity upon immobilization. It was further predicted via modeling that by incorporating 0.1 mM each of active GOx and CAT, the sensors will exhibit less than 2% variation in response over 1 month of continuous operation.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The average glucose level of tears for fasting diabetic patients typically exceeds 0.9 mM, and for fasting people without diabetes it is below 0.6 mM.
“Because the average glucose level of tears for fasting diabetic patients typically exceeds 0.9 mM and the concentration for fasting people without diabetes is below 0.6 mM (39)”
From Soft, smart contact lenses with integrations of wireless circuits, glucose sensors, and displays (2018) · cites this paper as [39]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Nonlinear Parabolic and Elliptic Equations
Pao, C. V. (1993) · Springer eBooks · doi:10.1007/978-1-4615-3034-3 · Cited by 1,976 · see citing works
Topics in Fluorescence Spectroscopy
Lakowicz, J. R. (2003) · Kluwer Academic Publishers eBooks · doi:10.1007/0-306-47947-8 · Cited by 1,581 · see citing works
Klonoff, D. C. (2005) · Diabetes Care · doi:10.2337/diacare.28.5.1231 · Cited by 725 · see citing works
Fluorescence-based glucose sensors
Pickup, J. C., Hussain, F., Evans, N. D., Rolinski, O. J., & Birch, D. J. S. (2005) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2004.10.002 · Cited by 616 · see citing works
Kinetics and Mechanism of Action of Glucose Oxidase
Gibson, Q. H., SWOBODA, B. E. P., & Massey, V. (1964) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)91224-x · Cited by 539 · see citing works
Synthesis of ordered large pore SBA-15 spherical particles for adsorption of biomolecules
Katiyar, A., Yadav, S. K., Smirniotis, P. G., et al. (2006) · Journal of Chromatography A · doi:10.1016/j.chroma.2006.04.055 · Cited by 308 · see citing works
Stücker, M., Struk, A., Altmeyer, P. J., Herde, M., Baumgärtl, H., & Lübbers, D. W. (2002) · The Journal of Physiology · doi:10.1113/jphysiol.2001.013067 · Cited by 244 · see citing works
In vivo glucose monitoring: the clinical reality and the promise
Pickup, J. C., Hussain, F., Evans, N. D., & Sachedina, N. (2004) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2004.08.016 · Cited by 203 · see citing works
Stuart, D. A., Yonzon, C. R., Zhang, X., Lyandres, O., Shah, N. C., Glucksberg, M. R., Walsh, J. T., & Duyne, R. P. V. (2005) · Analytical Chemistry · doi:10.1021/ac0501238 · Cited by 182 · see citing works
The Effect of Hydrogen Peroxide on Glucose Oxidase from Aspergillus niger*
Kleppe, K. (1966) · Biochemistry · doi:10.1021/bi00865a018 · Cited by 171 · see citing works
Reversible inhibition and irreversible inactivation of catalase in presence of hydrogen peroxide
Lardinois, O. M., Mestdagh, M. M., & Rouxhet, P. G. (1996) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(96)00043-x · Cited by 146 · see citing works
Time‐dependent inactivation of immobilized glucose oxidase and catalase
Tse, P. H. S., & Gough, D. A. (1987) · Biotechnology and Bioengineering · doi:10.1002/bit.260290607 · Cited by 95 · see citing works
Amerov, A. K., Chen, J., Small, G. W., et al. (2005) · Analytical Chemistry · doi:10.1021/ac0504161 · Cited by 82 · see citing works
Stein, E. W., Grant, P. S., Zhu, H., & McShane, M. J. (2007) · Analytical Chemistry · doi:10.1021/ac061414z · Cited by 78 · see citing works
Self-citationDing, H. S., Hou, X., Hou, X. L., & Chance, B. (2005) · Journal of Biomedical Optics · doi:10.1117/1.1891345 · Cited by 71 · see citing works
Modeling of spherical fluorescent glucose microsensor systems: Design of enzymatic smart tattoos
Brown, J. Q., & McShane, M. J. (2005) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2005.08.013 · Cited by 66 · see citing works
Self-citationEnzymatic Fluorescent Microsphere Glucose Sensors:Evaluation of Response Under Dynamic Conditions
Brown, J. Q., Srivastava, R., Zhu, H., et al. (2006) · Diabetes Technology & Therapeutics · doi:10.1089/dia.2006.8.288 · Cited by 39 · see citing works
Stein, E. W., Singh, S. S., & McShane, M. J. (2008) · Analytical Chemistry · doi:10.1021/ac701738e · Cited by 38 · see citing works
Self-citationDual-wavelength polarimetry for monitoring glucose in the presence of varying birefringence
Wan, Q., Coté, G. L., & Dixon, J. B. (2005) · Journal of Biomedical Optics · doi:10.1117/1.1891175 · Cited by 35 · see citing works
Simultaneous co-immobilization of glucose oxidase and catalase in their substrates
Özyılmaz, G., & Tükel, S. S. (2007) · Applied Biochemistry and Microbiology · doi:10.1134/s000368380701005x · Cited by 31 · see citing works
Analysis: Immobilized Glucose Oxidase in Implantable Glucose Sensor Technology
Gough, D. A., & Bremer, T. M. (2000) · Diabetes Technology & Therapeutics · doi:10.1089/15209150050194242 · Cited by 30 · see citing works
Dynamic concentration challenges for biosensor characterization
Baker, D. A., & Gough, D. (1993) · Biosensors and Bioelectronics · doi:10.1016/0956-5663(93)80028-n · Cited by 5 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Soft, smart contact lenses with integrations of wireless circuits, glucose sensors, and displays
Park, J., Kim, J., Kim, S.-Y., Cheong, W. H., Jang, J., Park, Y.-G., Na, K., Kim, Y.-T., Heo, J. H., Lee, C. Y., et al. (2018) · Science Advances · doi:10.1126/sciadv.aap9841 · Cited by 671 · see citing works
- Authors
- Singh, S., & McShane, M.
- Affiliations
- Texas A&M University (US)
- ORCID
- Singh, Saurabh
- Venue
- Biosensors and Bioelectronics
- Publisher
- Elsevier BV
- Published
- 2010-01
- Volume
- 25
- Issue
- 5
- Pages
- 1075-1081
- ISSN
- 0956-5663 (ISSN-L)
- PMC
- PMC2818267
- OpenAlex
- W1964611095
- Cited by
- 35
- References
- 22
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 2 · 2021: 2 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 4 · 2012: 5
- FWCI
- 4.23
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biosensors and Bioelectronics (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Institute of Biomedical Imaging and Bioengineering (R01EB000739)
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Physical Sciences › Chemical Engineering › Bioengineering › Analytical Chemistry and Sensors
- Keywords
- glucose sensor, glucose oxidase, catalase, enzyme co-immobilization, enzyme degradation, implantable sensors, continuous glucose monitoring, oxygen scavenging
- MeSH terms
- Catalase, Computer Simulation, Equipment Design, Glucose, Glucose Oxidase, Luminescent Measurements, Microspheres, Models, Chemical, Sensitivity and Specificity, Reproducibility of Results, Biosensing Techniques, Computer-Aided Design, Equipment Failure Analysis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
