The DOI matches its Crossref record; 166 of the 166 references it cites were found in a registry or an index.
Laccase engineering: From rational design to directed evolution
Open accessAbstract
Laccases are multicopper oxidoreductases considered by many in the biotechonology field as the ultimate "green catalysts". This is mainly due to their broad substrate specificity and relative autonomy (they use molecular oxygen from air as an electron acceptor and they only produce water as by-product), making them suitable for a wide array of applications: biofuel production, bioremediation, organic synthesis, pulp biobleaching, textiles, the beverage and food industries, biosensor and biofuel cell development. Since the beginning of the 21st century, specific features of bacterial and fungal laccases have been exhaustively adapted in order to reach the industrial demands for high catalytic activity and stability in conjunction with reduced production cost. Among the goals established for laccase engineering, heterologous functional expression, improved activity and thermostability, tolerance to non-natural media (organic solvents, ionic liquids, physiological fluids) and resistance to different types of inhibitors are all challenges that have been met, while obtaining a more comprehensive understanding of laccase structure-function relationships. In this review we examine the most significant advances in this exciting research area in which rational, semi-rational and directed evolution approaches have been employed to ultimately convert laccases into high value-added biocatalysts.
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Cites
Multicopper oxidases and oxygenases
Solomon, E. I., Sundaram, U. M., & Machonkin, T. E. (1996) · Chemical Reviews · doi:10.1021/cr950046o · Cited by 3,664
Fungal laccases – occurrence and properties
Baldrian, P. (2006) · FEMS Microbiology Reviews · doi:10.1111/j.1574-4976.2005.00010.x · Cited by 2,179
Laccase: new functions for an old enzyme
Mayer, A. (2002) · Phytochemistry · doi:10.1016/s0031-9422(02)00171-1 · Cited by 1,358
Industrial and biotechnological applications of laccases: A review
Rodríguez Couto, S., & Toca Herrera, J. L. (2006) · Biotechnology Advances · doi:10.1016/j.biotechadv.2006.04.003 · Cited by 1,296
Laccases: blue enzymes for green chemistry
Riva, S. (2006) · Trends in Biotechnology · doi:10.1016/j.tibtech.2006.03.006 · Cited by 1,221
Protein stability promotes evolvability
Bloom, J. D., Labthavikul, S. T., Otey, C. R., & Arnold, F. H. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0510098103 · Cited by 1,211
Laccases: a never-ending story
Giardina, P., Faraco, V., Pezzella, C., Piscitelli, A., Vanhulle, S., & Sannia, G. (2010) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-009-0169-1 · Cited by 1,007
Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes
Reetz, M. T., & Carballeira, J. D. (2007) · Nature Protocols · doi:10.1038/nprot.2007.72 · Cited by 860
Laccases: A Useful Group of Oxidoreductive Enzymes
Gianfreda, L., Xu, F., & Bollag, J.-M. (1999) · Bioremediation Journal · doi:10.1080/10889869991219163 · Cited by 826
Laccases and their natural mediators: Biotechnological tools for sustainable eco-friendly processes
Cañas, A. I., & Camarero, S. (2010) · Biotechnology Advances · doi:10.1016/j.biotechadv.2010.05.002 · Cited by 710
Cited by
Laccases: structure, function, and potential application in water bioremediation
Arregui, L., Ayala, M., Gómez-Gil, X., Gutiérrez-Soto, G., Hernández-Luna, C. E., Herrera de los Santos, M., Levin, L., Rojo-Domínguez, A., Romero-Martínez, D., Saparrat, M. C. N., et al. (2019) · Microbial Cell Factories · doi:10.1186/s12934-019-1248-0 · Cited by 515
Biomass-based biorefineries: An important architype towards a circular economy
Kumar, B., & Verma, P. (2021) · Fuel · doi:10.1016/j.fuel.2020.119622 · Cited by 312
Aptitude of Oxidative Enzymes for Treatment of Wastewater Pollutants: A Laccase Perspective
Unuofin, J. O., Okoh, A. I., & Nwodo, U. U. (2019) · Molecules · doi:10.3390/molecules24112064 · Cited by 172
Fungal laccases as tools for biodegradation of industrial dyes
Zucca, P., Cocco, G., Sollai, F., & Sanjust, E. (2016) · Biocatalysis · doi:10.1515/boca-2015-0007 · Cited by 75
Comparison of different microbial laccases as tools for industrial uses
Tonin, F., Melis, R., Cordes, A., Sanchez-Amat, A., Pollegioni, L., & Rosini, E. (2016) · New Biotechnology · doi:10.1016/j.nbt.2016.01.007 · Cited by 65
Metadata
- Authors
- Mate, D. M., & Alcalde, M.
- Venue
- Biotechnology Advances
- Publisher
- Elsevier BV
- Published
- 2015-01
- Volume
- 33
- Issue
- 1
- Pages
- 25-40
- ISSN
- 0734-9750 (ISSN-L)
- OpenAlex
- W2014497823
- Cited by
- 364
- References
- 166
- FWCI
- 20.18
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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