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Multi-scale simulations of MUT-16 scaffold protein phase separation and client recognition

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Gaurav, Kumar, Busetto, Virginia, Páez-Moscoso, Diego Javier, Changiarath, Arya, Hanson, Sonya M., Falk, Sebastian, Ketting, René F., Stelzl, Lukas S.·2025·Biophysical Journal
DOI 10.1016/j.bpj.2025.08.001PMC PMC12709416

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Abstract

Abstract Phase separation of proteins plays a critical role in cellular organisation. How phase separated protein condensates underpin biological function and how condensates achieve specificity remain elusive. We investigated the phase separation of MUT-16, a scaffold protein in Mutator foci , and its role in recruiting the client protein MUT-8, a key component in RNA silencing in C. elegans . We employed a multiscale approach that combined coarse-grained (residue-level CALVADOS2 and near-atomistic Martini3) and atomistic simulations. Simulations across different resolutions provide a consistent perspective on how MUT-16 condensates recruit MUT-8, enabling the fine-tuning of chemical details while balancing the computational cost. Both coarse-grained models (CALVADOS2 and Martini3) predicted the relative phase separation propensities of MUT-16’s disordered regions, which we confirmed through in vitro experiments. Simulations also identified key sequence features and residues driving phase separation while revealing differences in residue interaction propensities between CALVADOS2 and Martini3. Furthermore, Martini3 and 350 µs atomistic simulations on Folding@Home of MUT-8’s N-terminal prion-like domain with MUT-16 M8BR cluster highlighted the importance of cation- π interactions between Tyr residues of MUT-8 and Arg residues of MUT-16 M8BR. Lys residues were observed to be more prone to interact in Martini3. Atomistic simulations revealed that the guanidinium group of Arg also engages in sp 2 - π interactions and hydrogen bonds with the backbone of Tyr, making Arg-Tyr interactions stronger than Lys-Tyr, where these additional favourable contacts are absent. In agreement with our simulations, in vitro co-expression pulldown experiments demonstrated a progressive loss of MUT-8 recruitment following the mutation of Arg in MUT-16 M8BR to Lys or Ala, confirming the critical role of Arg in this interaction. These findings advance our understanding of MUT-16 phase separation and subsequent MUT-8 recruitment, key processes in assembling Mutator foci that drive RNA silencing in C. elegans . Statement of Significance In cells proteins phase separate and form condensates. These protein condensates can play important role in bringing molecules together and facilitate biochemical processes. In this work, we used molecular dynamics simulations to understand how MUT-16 phase separates and forms the scaffold of the so-called Mutator focus . Mutator foci produce small RNA which help to regulates genes. As the scaffold of the Mutator focus , MUT-16 recruit multiple proteins which are important for the production of such small RNAs.

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Metadata

Authors
Gaurav, K., Busetto, V., Páez-Moscoso, D. J., Changiarath, A., Hanson, S. M., Falk, S., Ketting, R. F., & Stelzl, L. S.
Affiliations
  • Johannes Gutenberg University Mainz (DE)
  • University of Vienna (AT)
  • Institute of Molecular Biology (DE)
  • Flatiron Institute (US)
Corresponding author
Stelzl, Lukas S.
ORCID
Venue
Biophysical Journal
Publisher
Elsevier BV
Published
2025-11
Volume
124
Issue
22
Pages
3987-4004
ISSN
0006-3495 (ISSN-L)
DOI
10.1016/j.bpj.2025.08.001
PMC
PMC12709416
OpenAlex
W4413043124
Cited by
7
References
4
Citations by year
2026: 5 · 2025: 2
FWCI
2.10
Type
journal article
Language
en
Open access
Licence
Locations
  • Biophysical Journal (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • Gutenberg Open Science (submittedVersion, cc-by-nd)
  • Gutenberg Open Science (cc-by)
Funders
German Research Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Keywords
phase separation, protein-protein interaction, hydrogen bonding, multiscale modeling, RNA silencing, Caenorhabditis elegans
MeSH terms
Phase Separation, Animals, Protein Binding, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Molecular Dynamics Simulation

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