Temporal lobe epilepsy induces differential expression of hippocampal miRNAs including let-7e and miR-23a/b
Abstract
To understand the role of miRNAs in the molecular mechanisms of temporal lobe epilepsy (TLE), we investigated the changes in microRNA (miRNA) expression profiles of chronic TLE rat models. MiRNAs microarray analysis results showed that 125 miRNAs were detected in the hippocampus of lithium-pilocarpine-induced TLE rats and normal rats. Compared with normal rats (control group), 23 of the 125 miRNAs were expressed differentially in TLE rats including 5 down-regulated miRNAs (let-7 e included) and 18 up-regulated miRNAs (miR-23 a/b included). Furthermore, let-7 e and miR-23 a/b analysis in rat hippocampus were performed by real-time quantitative polymerase chain reaction at 0 h, 1h, 6h, 12h, 24h, 2 days, 7 days,10 days, 30 days,50 days after induction of status epilepticus (SE). let-7 e was detected down-regulated expression at 0 h, 1h, 6h, 2 days, 7 days, 50 days after SE and up-regulated expression at 12h, 24h, 10 days, 30 days after SE, which was significantly up-regulated expression at 24h after SE (10.49 folds, P<0.01). miR-23 a/b was detected down-regulated at 0 h, 1h, 6h, 12h, 2 days, 7 days, 10 days, 30 days after SE and significantly up-regulated at 24h (4.49 folds P<0.01), 50 d (2.4 folds, P<0.01) after SE. TLE alters the expression levels of a subset of miRNAs in the hippocampus and these deregulated miRNAs may be involved in the pathogenesis of epilepsy directly or indirectly. Also the temporal change of the let-7 e and miR-23 a/b expression in the epileptogenesis in…
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In a chronic temporal lobe epilepsy (TLE) rat model, the expression levels of miR-23a/b were upregulated and dysregulated with status epilepticus in the hippocampus.
“in a chronic temporal lobe epilepsy (TLE) rat model, the expression levels of miR-23a/b were upregulated and dysregulated with status epilepticus in the hippocampus.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [136]
Intracerebral injection of miR-23a-3p inhibitors can reduce brain damage and the related learning and memory deficits in valproic acid (VPA) resistance rat models.
“Intracerebral injection of miR-23a-3p inhibitors can reduce brain damage and the related learning and memory deficits in valproic acid (VPA) resistance rat models.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [136]
Oatp2 is a direct target of miR-23a-3p.
“The influx drug transporter organic anion-transporting polypeptide 2 (Oatp2), is a direct target of miR-23a-3p.”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [136]
miR-23a-3p negatively regulates Oatp2 in brain microvascular endothelial cells (BMECs), leading to reduced VPA uptake in intractable epilepsy.
“The miR-23a-3p negatively regulates Oatp2 in the brain microvascular endothelial cells (BMECs), leading to reduced VPA uptake in intractable epilepsy [136,137].”
From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [136]
miRNA-146a is upregulated in experimental models of epilepsy and in human temporal lobe epilepsy.
“Interestingly, this miRNA has been shown to be upregulated in experimental models of epilepsy, as well as in human TLE [20]–[22].”
From MicroRNA-146a: A Key Regulator of Astrocyte-Mediated Inflammatory Response (2012) · cites this paper as [21]
miR-146a has been shown to be upregulated in experimental models of epilepsy and in human temporal lobe epilepsy.
“The miR-146a has recently been shown to be upregulated in experimental models of epilepsy, as well as in human TLE. [20], [21].”
From MicroRNA-146a: A Key Regulator of Astrocyte-Mediated Inflammatory Response (2012) · cites this paper as [21]
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References 24 · Cited by 135
- Authors
- Song, Y.-J., Tian, X.-B., Zhang, S., Zhang, Y.-X., Li, X., Li, D., Cheng, Y., Zhang, J.-N., Kang, C.-S., & Zhao, W.
- Affiliations
- Tianjin Medical University General Hospital (CN)
- Corresponding author
- Zhao, Wen
- ORCID
- Cheng, Yan
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2011-04
- Volume
- 1387
- Pages
- 134-140
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W1995751132
- Cited by
- 135
- References
- 24
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 7 · 2023: 6 · 2022: 5 · 2021: 6 · 2020: 5 · 2019: 9 · 2018: 7 · 2017: 10 · 2016: 19 · 2015: 14 · 2014: 14 · 2013: 14 · 2012: 15
- FWCI
- 9.63
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Key Project of Science and Technology Supporting Programs of China (2007BAI05B08)
- China National Natural Scientific Fund (81071044, 30872969)
- Tianjin Natural Science Fund (10JCYBJC13800)
- Project of Science & Technology Developing Fund of Tianjin colleges and universities (20090123)
- Topics
- Health Sciences › Medicine › Psychiatry and Mental health › Epilepsy research and treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Pharmacological Effects and Toxicity Studies
- Keywords
- temporal lobe epilepsy, microRNA expression, hippocampus, let-7e, status epilepticus, lithium-pilocarpine model, epileptogenesis, differential gene expression, epilepsy pathogenesis
- MeSH terms
- Animals, Epilepsy, Temporal Lobe, Hippocampus, Male, Gene Expression, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs, Rats
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