Comparative neurotoxicity screening in human iPSC-derived neural stem cells, neurons and astrocytes
Open accessAbstract
Induced pluripotent stem cells (iPSC) and their differentiated derivatives offer a unique source of human primary cells for toxicity screens. Here, we report on the comparative cytotoxicity of 80 compounds (neurotoxicants, developmental neurotoxicants, and environmental compounds) in iPSC as well as isogenic iPSC-derived neural stem cells (NSC), neurons, and astrocytes. All compounds were tested over a 24-h period at 10 and 100 μM, in duplicate, with cytotoxicity measured using the MTT assay. Of the 80 compounds tested, 50 induced significant cytotoxicity in at least one cell type; per cell type, 32, 38, 46, and 41 induced significant cytotoxicity in iPSC, NSC, neurons, and astrocytes, respectively. Four compounds (valinomycin, 3,3',5,5'-tetrabromobisphenol, deltamethrin, and triphenyl phosphate) were cytotoxic in all four cell types. Retesting these compounds at 1, 10, and 100 μM using the same exposure protocol yielded consistent results as compared with the primary screen. Using rotenone, we extended the testing to seven additional iPSC lines of both genders; no substantial difference in the extent of cytotoxicity was detected among the cell lines. Finally, the cytotoxicity assay was simplified by measuring luciferase activity using lineage-specific luciferase reporter iPSC lines which were generated from the parental iPSC line. This article is part of a Special Issue entitled SI: PSC and the brain.
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An acute inflammatory response might be triggered, activating microglia to produce cytokines and free radicals that could further harm neural cells.
“Additionally, an acute inflammatory response might be triggered, activating microglia, the resident immune cells in the brain, to produce cytokines and free radicals that could further harm neural cells (Pei et al., 2016).”
From Comparative analysis of energy transfer mechanisms for neural implants (2024) · cites this paper as Pei et al., 2016
The Introduction discusses susceptibility to environmental influences, and the Results report cytotoxicity in iPSC-derived neural cells, but neither addresses an acute inflammatory response. Searches of our copy for microglia, cytokines, and inflammation found no passage describing microglial activation or release of cytokines and free radicals. (LLM, conf=0.76)
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Identification of Selective Inhibitors of Cancer Stem Cells by High-Throughput Screening
Gupta, P. B., Önder, T. T., Jiang, G., Tao, K. X., Kuperwasser, C., Weinberg, R. A., & Lander, E. S. (2009) · Cell · doi:10.1016/j.cell.2009.06.034 · Cited by 2,393 · see citing works
Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction
Berridge, M. V., Herst, P. M., & Tan, A. S. (2005) · Biotechnology Annual Review · doi:10.1016/s1387-2656(05)11004-7 · Cited by 2,222 · see citing works
Neurobehavioural effects of developmental toxicity
Grandjean, P., & Landrigan, P. J. (2014) · The Lancet Neurology · doi:10.1016/s1474-4422(13)70278-3 · Cited by 1,886 · see citing works
Quantitative growth and development of human brain
Dobbing, J., & Sands, J. (1973) · Archives of Disease in Childhood · doi:10.1136/adc.48.10.757 · Cited by 1,442 · see citing works
Extrapolating brain development from experimental species to humans
Clancy, B., Finlay, B. L., Darlington, R. B., & Anand, K. J. S. (2007) · NeuroToxicology · doi:10.1016/j.neuro.2007.01.014 · Cited by 812 · see citing works
A highly reproducible rotenone model of Parkinson's disease
Cannon, J. R., Tapias, V., Na, H. M., Honick, A. S., Drolet, R. E., & Greenamyre, J. T. (2009) · Neurobiology of Disease · doi:10.1016/j.nbd.2009.01.016 · Cited by 802 · see citing works
Transforming Environmental Health Protection
Collins, F. S., Gray, G. M., & Bucher, J. R. (2008) · Science · doi:10.1126/science.1154619 · Cited by 630 · see citing works
Mechanism of 6-hydroxydopamine neurotoxicity
Glinka, Y., Gassen, M., & Youdim, M. B. H. (1997) · Journal of neural transmission. Supplementum · doi:10.1007/978-3-7091-6842-4_7 · Cited by 381 · see citing works
Tetrazolium (MTT) Assay for Cellular Viability and Activity
Morgan, D. M. L. (2003) · Humana Press eBooks · doi:10.1385/0-89603-448-8:179 · Cited by 339 · see citing works
A Review of the Toxicology of Acrylamide
Exon, J. H. (2006) · Journal of Toxicology and Environmental Health Part B · doi:10.1080/10937400600681430 · Cited by 321 · see citing works
Swistowski, A., Peng, J., Liu, Q., Mali, P., Rao, M. S., Cheng, L., & Zeng, X. (2010) · Stem Cells · doi:10.1002/stem.499 · Cited by 317 · see citing works
Self-citationEffects of developmental exposure to bisphenol A on brain and behavior in mice
Palanza, P., Gioiosa, L., Saal, F. S. V., & Parmigiani, S. (2008) · Environmental Research · doi:10.1016/j.envres.2008.07.023 · Cited by 276 · see citing works
Efficient Generation of Astrocytes from Human Pluripotent Stem Cells in Defined Conditions
Shaltouki, A., Peng, J., Liu, Q., Rao, M. S., & Zeng, X. (2013) · Stem Cells · doi:10.1002/stem.1334 · Cited by 264 · see citing works
Self-citationCompound Cytotoxicity Profiling Using Quantitative High-Throughput Screening
Xia, M., Huang, R., Witt, K. L., Southall, N. T., Fostel, J. M., Cho, M.-H., Jadhav, A., Smith, C. S., Inglese, J., Portier, C. J., et al. (2007) · Environmental Health Perspectives · doi:10.1289/ehp.10727 · Cited by 256 · see citing works
Self-citationZou, J., Sweeney, C. L., Chou, B.-K., Choi, U., Pan, J., Wang, H., Dowey, S. N., Cheng, L., & Malech, H. L. (2011) · Blood · doi:10.1182/blood-2010-12-328161 · Cited by 234 · see citing works
Neurotoxicity of Acrylamide in Exposed Workers
Pennisi, M., Malaguarnera, G., Puglisi, V., Vinciguerra, L., Vacante, M., & Malaguarnera, M. (2013) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph10093843 · Cited by 234 · see citing works
Segregation of Human Neural Stem Cells in the Developing Primate Forebrain
Ourednik, V., Ourednik, J., Flax, J. D., Zawada, W. M., Hutt, C., Yang, C., Park, K. I., Kim, S. U., Sidman, R. L., Freed, C. R., et al. (2001) · Science · doi:10.1126/science.1060580 · Cited by 230 · see citing works
Drug discovery in dementia: the role of rodent models
Dam, D. V., & Deyn, P. P. D. (2006) · Nature Reviews Drug Discovery · doi:10.1038/nrd2075 · Cited by 220 · see citing works
Richardson, J. R., Caudle, W. M., Wang, M., Dean, E. D., Pennell, K. D., & Miller, G. W. (2006) · The FASEB Journal · doi:10.1096/fj.06-5864fje · Cited by 215 · see citing works
Rinaldi, T. (2008) · Frontiers in Neural Circuits · doi:10.3389/neuro.04.004.2008 · Cited by 210 · see citing works
Erkekoğlu, P., & Baydar, T. (2013) · Nutritional Neuroscience · doi:10.1179/1476830513y.0000000065 · Cited by 207 · see citing works
Dieldrin-Induced Neurotoxicity: Relevance to Parkinson's Disease Pathogenesis
Kanthasamy, A. G., Kitazawa, M., Kanthasamy, A., & Anantharam, V. (2004) · NeuroToxicology · doi:10.1016/j.neuro.2004.07.010 · Cited by 201 · see citing works
Mustafa, S., Walker, A. J., Bennett, G., & Wigmore, P. M. (2008) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2008.06325.x · Cited by 193 · see citing works
Human stem cells and drug screening: opportunities and challenges
Ebert, A., & Svendsen, C. (2010) · Nature reviews. Drug discovery · doi:10.1038/nrd3000 · Cited by 178 · see citing works
Yen, J. H., Donerly, S., Levin, E. D., & Linney, E. A. (2011) · Neurotoxicology and Teratology · doi:10.1016/j.ntt.2011.10.004 · Cited by 170 · see citing works
Crofton, K. M. (2010) · ALTEX · doi:10.14573/altex.2011.1.009 · Cited by 169 · see citing works
Evaluation of a human neurite growth assay as specific screen for developmental neurotoxicants
Krug, A. K., Balmer, N. V., Matt, F., Schönenberger, F., Merhof, D., & Leist, M. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1072-y · Cited by 169 · see citing works
Hu, B.-Y., Du, Z.-W., Li, X.-J., Ayala, M., & Zhang, S.-C. (2009) · Development · doi:10.1242/dev.029447 · Cited by 169 · see citing works
Yücel, M., Takagi, M., Walterfang, M., & Lubman, D. I. (2008) · Neuroscience & Biobehavioral Reviews · doi:10.1016/j.neubiorev.2008.01.006 · Cited by 162 · see citing works
Methyl Parathion: A Review of Health Effects
Garcia, S. J., Abu‐Qare, A. W., Meeker-O’Connell, W. A., Borton, A. J., & Abou-Donia, M. B. (2003) · Journal of Toxicology and Environmental Health Part B · doi:10.1080/10937400306471 · Cited by 158 · see citing works
Chlorpyrifos exerts opposing effects on axonal and dendritic growth in primary neuronal cultures
HOWARD, A., Bucelli, R. C., Jett, D. A., Bruun, D. A., YANG, D., & Lein, P. J. (2005) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2004.12.008 · Cited by 155 · see citing works
Vorhees, C. V., Butcher, R. E., Brunner, R. L., & Sobotka, T. J. (1979) · Toxicology and Applied Pharmacology · doi:10.1016/0041-008x(79)90152-2 · Cited by 153 · see citing works
Swistowski, A., Peng, J., Han, Y., Swistowska, A. M., Rao, M. S., & Zeng, X. (2009) · PLoS ONE · doi:10.1371/journal.pone.0006233 · Cited by 144 · see citing works
Self-citationMOSER, V. C. (1995) · Neurotoxicology and Teratology · doi:10.1016/0892-0362(95)02002-0 · Cited by 142 · see citing works
Thalidomide sensory neurotoxicity
Cavaletti, G., Beronio, A., Reni, L., Ghiglione, E., Schenone, A. E., Briani, C., Zara, G., Cocito, D., Isoardo, G., Ciaramitaro, P., et al. (2004) · Neurology · doi:10.1212/wnl.62.12.2291 · Cited by 135 · see citing works
Lindane toxicity: a comprehensive review of the medical literature.
Nolan, K., Kamrath, J., & Levitt, J. (2012) · Pediatric Dermatology · doi:10.1111/j.1525-1470.2011.01519.x · Cited by 130 · see citing works
Eriksson, P., & Fredriksson, A. (1991) · Toxicology and Applied Pharmacology · doi:10.1016/0041-008x(91)90270-o · Cited by 119 · see citing works
The Toxicologic Effects of the Carbamate Insecticide Aldicarb in Mammals: A Review
Risher, J. F., Mink, F. L., & Stara, J. F. (1987) · Environmental Health Perspectives · doi:10.1289/ehp.8772267 · Cited by 116 · see citing works
Win‐Shwe, T., & Fujimaki, H. (2010) · Toxicology Letters · doi:10.1016/j.toxlet.2010.06.022 · Cited by 114 · see citing works
Investigating monogenic and complex diseases with pluripotent stem cells
Zhu, H., Lensch, M. W., Cahan, P., & Daley, G. (2011) · Nature reviews genetics · doi:10.1038/nrg2951 · Cited by 112 · see citing works
Chaudhary, S., & Parvez, S. (2012) · Neuroscience · doi:10.1016/j.neuroscience.2012.08.060 · Cited by 112 · see citing works
Gilbert, M. E., & Paczkowski, C. (2003) · Developmental Brain Research · doi:10.1016/s0165-3806(03)00191-3 · Cited by 111 · see citing works
Miyagawa, K., Narita, M., Narita, M., Akama, H., & Suzuki, T. (2007) · Neuroscience Letters · doi:10.1016/j.neulet.2007.01.088 · Cited by 108 · see citing works
Desai, V. G., Feuers, R. J., Hart, R. W., & Ali, S. F. (1996) · Brain Research · doi:10.1016/0006-8993(95)01255-9 · Cited by 105 · see citing works
Neurotoxicity of Hexachlorophene in the Human: I. A Clinicopathologic Study of 248 Children
Shuman, R. M., Leech, R. W., & Alvord, E. C. (1974) · PEDIATRICS · doi:10.1542/peds.54.6.689 · Cited by 105 · see citing works
Patisaul, H. B., Fortino, A. E., & Polston, E. K. (2006) · NeuroToxicology · doi:10.1016/j.neuro.2006.10.001 · Cited by 94 · see citing works
Axelstad, M., Hansen, P. R., Boberg, J., Bonnichsen, M., Nellemann, C., Lund, S. P., Hougaard, K. S., & Hass, U. (2008) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2008.05.020 · Cited by 88 · see citing works
Heptachlor alters expression and function of dopamine transporters.
Miller, G. W., Kirby, M. L., Levey, A. I., & Bloomquist, J. R. (1999) · PubMed · Cited by 86 · see citing works
Concentration of di(2-ethylhexyl) phthalate (DEHP) in foods and its dietary exposure in China
Sui, H., Zhang, L., Wu, P., Song, Y., Yong, L., Yang, D., Jiang, D., & Liu, Z. (2014) · International Journal of Hygiene and Environmental Health · doi:10.1016/j.ijheh.2014.02.006 · Cited by 86 · see citing works
MOSER, V. C. (2001) · Toxicological Sciences · doi:10.1093/toxsci/62.2.339 · Cited by 84 · see citing works
Shafer, T. J. (2008) · NeuroToxicology · doi:10.1016/j.neuro.2008.01.002 · Cited by 81 · see citing works
Azole Fungicides Disturb Intracellular Ca2+ in an Additive Manner in Dopaminergic PC12 Cells
Heusinkveld, H. J., Molendijk, J., van den Berg, M., & Westerink, R. H. S. (2013) · Toxicological Sciences · doi:10.1093/toxsci/kft119 · Cited by 79 · see citing works
Neurotoxicity of Hexachlorophene in Humans
Shuman, R. M. (1975) · Archives of Neurology · doi:10.1001/archneur.1975.00490470064009 · Cited by 78 · see citing works
Effects of Methylmercury and Mercuric Chloride on Differentiation and Cell Viability in PC12 Cells
Parran, D. K. (2001) · Toxicological Sciences · doi:10.1093/toxsci/59.2.278 · Cited by 75 · see citing works
Efthymiou, A. G., Shaltouki, A., Steiner, J. P., Jha, B. S., Heman-Ackah, S. M., Swistowski, A., Zeng, X., Rao, M. S., & Malik, N. (2013) · SLAS DISCOVERY · doi:10.1177/1087057113501869 · Cited by 70 · see citing works
Self-citationZeng, X., Chen, J., Sanchez, J. F., Coggiano, M. A., Dillon‐Carter, O., Petersen, J., & Freed, W. J. (2003) · Stem Cells · doi:10.1634/stemcells.21-6-647 · Cited by 70 · see citing works
Self-citationLate onset deficits in synaptic plasticity in the valproic acid rat model of autism
Martin, H. G. S., & Manzoni, O. J. (2014) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2014.00023 · Cited by 69 · see citing works
Hernandez, C. M., Beck, W. D., Naughton, S. X., Poddar, I., Adam, B.-L., Yanasak, N. E., Middleton, C., & Terry, A. V. (2015) · NeuroToxicology · doi:10.1016/j.neuro.2015.01.002 · Cited by 68 · see citing works
Levin, E. D., Timofeeva, O. A., Yang, L., Petro, A. E., Ryde, I. T., Wrench, N., Seidler, F. J., & Slotkin, T. A. (2009) · Behavioural Brain Research · doi:10.1016/j.bbr.2009.11.007 · Cited by 66 · see citing works
Peng, J., Liu, Q., Rao, M. S., & Zeng, X. (2013) · SLAS Discovery · doi:10.1177/1087057112474468 · Cited by 64 · see citing works
Self-citationMOSER, V. C. (1999) · Toxicology and Applied Pharmacology · doi:10.1006/taap.1999.8675 · Cited by 64 · see citing works
Early life permethrin exposure induces long-term brain changes in Nurr1, NF-kB and Nrf-2
Carloni, M., Nasuti, C., Fedeli, D., Montani, M., Vadhana, M. S. D., Amici, A. R. D., & Gabbianelli, R. (2013) · Brain Research · doi:10.1016/j.brainres.2013.03.048 · Cited by 60 · see citing works
Neurotoxicity of the Organochlorine Insecticide Heptachlor to Murine Striatal Dopaminergic Pathways
Kirby, M. L. (2001) · Toxicological Sciences · doi:10.1093/toxsci/61.1.100 · Cited by 60 · see citing works
What Makes the Human Brain Different?
Deacon, T. W. (1997) · Annual Review of Anthropology · doi:10.1146/annurev.anthro.26.1.337 · Cited by 59 · see citing works
Direct effects of manganese compounds on dopamine and its metabolite Dopac: An in vitro study
Sistrunk, S. C., Ross, M. K., & Filipov, N. M. (2006) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2006.11.004 · Cited by 59 · see citing works
Neurotoxic effects of colchicine.
Mundy, W. R., & Tilson, H. A. (1990) · PubMed · Cited by 57 · see citing works
Increased spontaneous transmitter release from presynaptic nerve terminal by methylmercuric chloride
Juang, M. S., & Yonemura, K. (1975) · Nature · doi:10.1038/256211a0 · Cited by 53 · see citing works
van Dartel, D. A. M., & Piersma, A. H. (2011) · Reproductive Toxicology · doi:10.1016/j.reprotox.2011.04.008 · Cited by 53 · see citing works
Johannessen, J. N. (1991) · PubMed · Cited by 52 · see citing works
Normandin, L., Panisset, M., & Zayed, J. (2002) · Reviews on Environmental Health · doi:10.1515/reveh.2002.17.3.189 · Cited by 51 · see citing works
Swistowska, A. M., da Cruz, A. B., Han, Y., Swistowski, A., Liu, Y., Shin, S., Zhan, M., Rao, M. S., & Zeng, X. (2009) · Stem Cells and Development · doi:10.1089/scd.2009.0107 · Cited by 51 · see citing works
Self-citationRogel‐Fuchs, Y., Newman, M. E., Trombka, D., Zahalka, E. A., & Yanai, J. (1992) · Brain Research Bulletin · doi:10.1016/0361-9230(92)90002-f · Cited by 50 · see citing works
Malik, N., Efthymiou, A. G., Mather, K., Chester, N., Wang, X., Nath, A., Rao, M. S., & Steiner, J. P. (2014) · NeuroToxicology · doi:10.1016/j.neuro.2014.10.007 · Cited by 50 · see citing works
Self-citationA platform for rapid generation of single and multiplexed reporters in human iPSC lines
Pei, Y., Sierra, G., Sivapatham, R., Swistowski, A., Rao, M. S., & Zeng, X. (2015) · Scientific Reports · doi:10.1038/srep09205 · Cited by 48 · see citing works
Self-citationNeurotoxicity and Behavioral Effects of Thiram in Rats
Lee, C. C., & Peters, P. J. (1976) · Environmental Health Perspectives · doi:10.1289/ehp.761735 · Cited by 48 · see citing works
Han, Y., Miller, A. W., Mangada, J., Liu, Y., Swistowski, A., Zhan, M., Rao, M. S., & Zeng, X. (2009) · PLoS ONE · doi:10.1371/journal.pone.0007155 · Cited by 43 · see citing works
Self-citationNeural and behavioral alterations after early exposure to phenobarbital.
Yanai, J., Fares, F., Gavish, M., Greenfeld, Z., Katz, Y. S., Marcovici, G., Cg, P., Rogel‐Fuchs, Y., & Weizman, A. (1989) · PubMed · Cited by 42 · see citing works
Stem Cells in the Nervous System
Maldonado‐Soto, A. R., Oakley, D. H., Wichterle, H., Stein, J. M., Doetsch, F. K., & Henderson, C. E. (2014) · American Journal of Physical Medicine & Rehabilitation · doi:10.1097/phm.0000000000000111 · Cited by 41 · see citing works
Developmental neurotoxicity following neonatal exposure to 3,3′-iminodipropionitrile in the rat
Crofton, K. M., Peele, D. B., & Stanton, M. E. (1993) · Neurotoxicology and Teratology · doi:10.1016/0892-0362(93)90070-5 · Cited by 39 · see citing works
Subchronic neurotoxicity of chlorpyrifos, carbaryl, and their combination in rats
Wang, H., Liang, Y., Sun, Y., Hou, W., Chen, J., Long, D., Xu, M., & Wu, Y. (2013) · Environmental Toxicology · doi:10.1002/tox.21851 · Cited by 39 · see citing works
Joy, R. M., & Albertson, T. E. (1987) · PubMed · Cited by 38 · see citing works
Shinomiya, N., & Shinomiya, M. (2003) · Toxicology Letters · doi:10.1016/s0378-4274(02)00401-0 · Cited by 38 · see citing works
MOSER, V. C. (1993) · Fundamental and Applied Toxicology · doi:10.1006/faat.1993.1100 · Cited by 38 · see citing works
Manganese Inhalation as a Parkinson Disease Model
Ordóñez-Librado, J. L., Anaya-Martı́nez, V., Gutiérrez-Valdez, A. L., Colín-Barenque, L., Montiel-Flores, E., & Ávila-Costa, M. R. (2010) · Parkinson s Disease · doi:10.4061/2011/612989 · Cited by 37 · see citing works
Hu, X.-D., Ge, Y.-X., Hu, N., Zhang, H., Zhou, L., Zhang, T., Li, W., Han, Y., & Liu, X. (2005) · Neuropharmacology · doi:10.1016/j.neuropharm.2005.09.010 · Cited by 35 · see citing works
Liu, Q., Pedersen, O. Z., Peng, J., Couture, L. A., Rao, M. S., & Zeng, X. (2013) · Cytotherapy · doi:10.1016/j.jcyt.2013.03.006 · Cited by 35 · see citing works
Self-citationNeurotoxicologic sequelae of tributyltin intoxication in rats
Elsabbagh, H. S., moussa, S. Z., & El-Tawil, O. S. (2002) · Pharmacological Research · doi:10.1006/phrs.2001.0909 · Cited by 34 · see citing works
Momčilović, O., Liu, Q., Swistowski, A., Russo‐Tait, T., Zhao, Y., Rao, M. S., & Zeng, X. (2013) · Stem Cells and Development · doi:10.1089/scd.2013.0412 · Cited by 33 · see citing works
Self-citationNELSON, B., Brightwell, W. S., Burg, J. R., & Massari, V. J. (1984) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(84)90254-5 · Cited by 33 · see citing works
Sachana, M., Flaskos, J., Alexaki, E., & Hargreaves, A. J. (2003) · Toxicology in Vitro · doi:10.1016/s0887-2333(02)00121-2 · Cited by 33 · see citing works
Rotenone potentiates NMDA currents in substantia nigra dopamine neurons
Wu, Y.-N., & Johnson, S. W. (2007) · Neuroscience Letters · doi:10.1016/j.neulet.2007.05.030 · Cited by 33 · see citing works
Gutherz, S. B., Kulick, C. V., Soper, C., Kondratyev, A. D., Gale, K., & Forcelli, P. A. (2014) · Epilepsy & Behavior · doi:10.1016/j.yebeh.2014.07.010 · Cited by 33 · see citing works
Wu, Y., Li, K., Zuo, H., Yuan, Y., Sun, Y., & Yang, X. (2014) · Journal of Environmental Sciences · doi:10.1016/s1001-0742(13)60504-5 · Cited by 33 · see citing works
A human pluripotent stem cell platform for assessing developmental neural toxicity screening
Hou, Z., Zhang, J., Schwartz, M. P., Stewart, R. M., Page, C. D., Murphy, W. L., & Thomson, J. A. (2013) · Stem Cell Research & Therapy · doi:10.1186/scrt373 · Cited by 31 · see citing works
Shiraki, A., Saito, F., Akane, H., Takeyoshi, M., Imatanaka, N., Itahashi, M., Yoshida, T., & Shibutani, M. (2014) · Toxicology Letters · doi:10.1016/j.toxlet.2014.04.018 · Cited by 30 · see citing works
Enantioselective Cytotoxicity Profile of o,p’-DDT in PC 12 Cells
Zhao, M., Wang, C., Zhang, C., Wen, Y., & Liu, W. (2012) · PLoS ONE · doi:10.1371/journal.pone.0043823 · Cited by 30 · see citing works
Wilkinson, M., & Herdon, H. J. (1982) · Brain Research · doi:10.1016/0006-8993(82)91149-0 · Cited by 30 · see citing works
Peng, J., Wu, Z. J., Wu, Y., Hsu, M., Stevenson, F. F., Boonplueang, R., Roffler-Tarlov, S. K., & Andersen, J. K. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m207407200 · Cited by 28 · see citing works
Self-citationLearning and memory deficits in rats following exposure to 3,3′-iminodipropionitrile
Peele, D. B., Allison, S. D., & Crofton, K. M. (1990) · Toxicology and Applied Pharmacology · doi:10.1016/0041-008x(90)90193-x · Cited by 27 · see citing works
Harrill, J., Li, Z., Wright, F. A., Radio, N. M., Mundy, W. R., Tornero‐Velez, R., & Crofton, K. M. (2008) · BMC Genomics · doi:10.1186/1471-2164-9-546 · Cited by 26 · see citing works
Neurotoxicity of MPTP and MPP+ in vitro: characterization using specific cell lines
Notter, M. F. D., Irwin, I., Langston, J. W., & Gash, D. M. (1988) · Brain Research · doi:10.1016/0006-8993(88)90225-9 · Cited by 24 · see citing works
Neurobehavioral teratogenic effects of thalidomide in rats
Vorhees, C. V., Weisenburger, W. P., & Minck, D. R. (2001) · Neurotoxicology and Teratology · doi:10.1016/s0892-0362(01)00140-4 · Cited by 23 · see citing works
Effects of ethylene glycol ethers on cell viability in the human neuroblastoma SH-SY5Y cell line
Regulska, M., Pomierny, B., Basta‐Kaim, A., Starek, A., Filip, M., Lasoń, W., & Budziszewska, B. (2010) · Pharmacological Reports · doi:10.1016/s1734-1140(10)70389-3 · Cited by 23 · see citing works
Chlorpyrifos developmental neurotoxicity: Interaction with glucocorticoids in PC12 cells
Slotkin, T. A., Card, J., & Seidler, F. J. (2012) · Neurotoxicology and Teratology · doi:10.1016/j.ntt.2012.07.002 · Cited by 22 · see citing works
Concise Review: Modeling Central Nervous System Diseases Using Induced Pluripotent Stem Cells
Zeng, X., Hunsberger, J. G., Simeonov, A. M., Malik, N., Pei, Y., & Rao, M. S. (2014) · Stem Cells Translational Medicine · doi:10.5966/sctm.2014-0102 · Cited by 22 · see citing works
Self-citationCooper, J. F., & Kusnecov, A. (2007) · Neuroscience · doi:10.1016/j.neuroscience.2007.07.034 · Cited by 20 · see citing works
Itahashi, M., Abe, H., Tanaka, T., Mizukami, S., Kimura, M., Yoshida, T., & Shibutani, M. (2014) · Toxicology · doi:10.1016/j.tox.2014.12.009 · Cited by 20 · see citing works
Kaitsuka, T., Fukunaga, K., Soeda, F., Shirasaki, T., Miyamoto, E., & Takahama, K. (2006) · Neuroscience · doi:10.1016/j.neuroscience.2006.10.051 · Cited by 19 · see citing works
The toxicity of hexanedione isomers in neural and astrocytic cell lines
Woehrling, E. K., Zilz, T. R., & Coleman, M. D. (2006) · Environmental Toxicology and Pharmacology · doi:10.1016/j.etap.2005.12.010 · Cited by 16 · see citing works
Lie, K. H., Chung, H. C. Y., & Sidhu, K. (2012) · Methods in molecular biology · doi:10.1007/978-1-61779-794-1_15 · Cited by 15 · see citing works
Adigun, A. A., Ryde, I. T., Seidler, F. J., & Slotkin, T. A. (2010) · Brain Research · doi:10.1016/j.brainres.2010.03.025 · Cited by 15 · see citing works
Qin, X., Akanuma, H., Wei, F., Nagano, R., Zeng, Q., Imanishi, S., Ohsako, S., Yoshinaga, J., Yonemoto, J., Tanokura, M., et al. (2012) · NeuroToxicology · doi:10.1016/j.neuro.2012.08.016 · Cited by 13 · see citing works
Mechanisms and Models of Neurotoxicity of n-Hexane and Related Solvents
Tähti, H., Engelke, M., & Vaalavirta, L. (1997) · Archives of toxicology. Supplement · doi:10.1007/978-3-642-60682-3_32 · Cited by 13 · see citing works
Boffa, M. J., Brough, P. A., & Ead, R. D. (1995) · British Journal of Dermatology · doi:10.1111/j.1365-2133.1995.tb06950.x · Cited by 12 · see citing works
Testing for Carcinogens: Shift From Animals to Automation Gathers Steam—Slowly
Schmidt, C. (2009) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/djp191 · Cited by 12 · see citing works
n-Hexane neurotoxicity in metal can manufacturing workers
Bachmann, M. O., Beer, Z. D., & Myers, J. E. (1993) · Occupational Medicine · doi:10.1093/occmed/43.3.149 · Cited by 12 · see citing works
5-fluorouracil-induced histopathological changes in the central nervous system of rat fetuses.
Yamaguchi, Y., Aoki, A., Fukunaga, Y., Matsushima, K., Ebata, T., Ikeya, M., & Tamura, K. (2009) · PubMed · doi:10.14670/hh-24.133 · Cited by 10 · see citing works
MacPhail, R. C., Farmer, J. D., & Jarema, K. A. (2011) · NeuroToxicology · doi:10.1016/j.neuro.2011.12.006 · Cited by 10 · see citing works
The neurotoxic actions of 6-hydroxydopamine infused into the rat substantia nigra
Bradbury, A. J., Costall, B., Domeney, A. M., Jenner, P. G., MARSDEN, C. D., & Naylor, R. J. (1986) · Neuroscience Letters · doi:10.1016/0304-3940(86)90399-x · Cited by 10 · see citing works
Ferguson, C., & Audesirk, G. J. (1990) · Toxicology in Vitro · doi:10.1016/0887-2333(90)90005-e · Cited by 9 · see citing works
Neurotoxicity of hexachlorophene in mice: Implications of altered cerebral amino acid profiles
Prasad, G., Rajendra, W., & Indira, K. (1987) · Archives of Environmental Contamination and Toxicology · doi:10.1007/bf01055820 · Cited by 9 · see citing works
Effects of Diethylstilbestrol on Mouse Hippocampal Evoked Potentials in Vitro
Martín, S. S., Gutiérrez, M., Menéndez, L., Hidalgo, A., & Baamonde, A. (1999) · Cellular and Molecular Neurobiology · doi:10.1023/a:1006996805017 · Cited by 8 · see citing works
Neurochemical and Behavioural Effects of Chronic Aldicarb Administration in Rats
Palumbo, G., Bacchi, S., Coppolino, M. F., & Pantaleoni, G. C. (2001) · Pharmacology & Toxicology · doi:10.1034/j.1600-0773.2001.d01-155.x · Cited by 8 · see citing works
Neurotoxizität von 5-Fluorouracil
Sucker, C., Lambers, C., Stockschläder, M., & Dölken, G. (2002) · DMW - Deutsche Medizinische Wochenschrift · doi:10.1055/s-2002-34361 · Cited by 8 · see citing works
Ouardouz, M., & Sastry, B. R. (2005) · Neuroscience Letters · doi:10.1016/j.neulet.2005.09.050 · Cited by 8 · see citing works
Neurochemical and behavioural effects of diazepam: evidences from animal models.
Batool, F., & Haleem, D. J. (1997) · PubMed · Cited by 5 · see citing works
Astrocytes: A possible primary site for colchicine‐mediated neurotoxicity in the rat striatum
Pitts, O. M., Cohen, M. R., McAmis, W., & Gutman, R. L. (1991) · Glia · doi:10.1002/glia.440040114 · Cited by 5 · see citing works
Guan, Z., Wang, X., Dong, Y., Xu, L., Zhu, Z., Wang, J., Zhang, T., & Niu, B. (2014) · Toxicology · doi:10.1016/j.tox.2014.12.001 · Cited by 5 · see citing works
Zaniani, N. R., Karami, M., & Porkhodadad, S. (2013) · Europe PMC (PubMed Central) · Cited by 3 · see citing works
Schnare, S. N., & Lenzer, I. I. (1978) · Psychological Reports · doi:10.2466/pr0.1978.42.3.1007 · Cited by 1 · see citing works
[Effect of bis(n-tributyltin)-oxide on the brain GABA-ergic system in vitro].
Gerasimiak, G. R., Rozanov, V. A., & Shafran, L. M. (1994) · PubMed · Cited by 1 · see citing works
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Research works printed but not linked: 1
- Wood PL Alexander RS Felty CC 2014 Disulfiram Neurotoxicity: Decrements in Ethanolamine, Serine, and Inositol Glycerophospholipids Journal of Molecular Pathophysiology 3 33 37
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The HD iPSC Consortium, Lim, R. G., Salazar, L. L., Wilton, D. K., King, A. R., Stocksdale, J. T., Sharifabad, D., Lau, A. L., Stevens, B., Reidling, J. C., et al. (2017) · Nature Neuroscience · doi:10.1038/nn.4532 · Cited by 221 · see citing works
Complex Tissue and Disease Modeling using hiPSCs
Passier, R., Orlova, V., & Mummery, C. (2016) · Cell Stem Cell · doi:10.1016/j.stem.2016.02.011 · Cited by 142 · see citing works
Comparative analysis of energy transfer mechanisms for neural implants
Miziev, S., Pawlak, W. A., & Howard, N. (2024) · Frontiers in Neuroscience · doi:10.3389/fnins.2023.1320441 · Cited by 21 · see citing works
Human iPSC Models in Drug Discovery: Opportunities and Challenges
Silva, M. C., Cross, A., Brandon, N. J., & Haggarty, S. J. (2017) · Comprehensive Medicinal Chemistry III · doi:10.1016/b978-0-12-409547-2.12284-x · Cited by 6 · see citing works
- Authors
- Pei, Y., Peng, J., Behl, M., Sipes, N. S., Shockley, K. R., Rao, M. S., Tice, R. R., & Zeng, X.
- Affiliations
- XCell Science (United States) (US)
- Buck Institute for Research on Aging (US)
- National Institutes of Health (US)
- Corresponding author
- Zeng, Xianmin
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 1638
- Issue
- Pt A
- Pages
- 57-73
- ISSN
- 0006-8993 (ISSN-L)
- PMC
- PMC5032144
- OpenAlex
- W1055875683
- Cited by
- 128
- References
- 131
- Citations by year
- 2026: 6 · 2025: 7 · 2024: 10 · 2023: 7 · 2022: 8 · 2021: 14 · 2020: 14 · 2019: 12 · 2018: 21 · 2017: 13 · 2016: 11 · 2015: 3 · 2014: 1
- FWCI
- 6.95
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIEHS (1R43ES023522-01)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Veterinary › Small Animals › Animal testing and alternatives
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Keywords
- neurotoxicity screening, induced pluripotent stem cells, iPSC-derived neurons, iPSC-derived astrocytes, cytotoxicity, environmental chemicals, valinomycin, deltamethrin, triphenyl phosphate, rotenone, sex differences, MTT assay
- MeSH terms
- Astrocytes, Cell Differentiation, Cell Line, Cells, Cultured, Drug Evaluation, Preclinical, Humans, Neurons, Neurotoxicity Syndromes, Induced Pluripotent Stem Cells, Neural Stem Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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