The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Colorectal cancer cell detection by 5-aminolaevulinic acid-loaded chitosan nano-particles
Abstract
Colorectal cancer is one of the leading causes of malignant death in Taiwan because it often remains undetected until later stages of the disease. In this study, we designed an oral form nano-particle to encapsulate 5-aminolaevulinic acid (5-ALA) to improve the detection of colorectal cancer cells in vivo. The nano-particle should escape from bacteria uptake in the gastrointestinal tract which seriously interferes the results of endoscopic observation. In this study, chitosan was mixed with sodium tripolyphosphate (STPP) and 5-ALA to prepare chitosan nano-particles (CN) and 5-ALA loaded chitosan nano-particles (CNA) by adding different pH values and concentrations of 5-ALA solution. The average particle size and zeta-potential of CN and CNA were measured by the Zetasizer-3000. The results revealed that particle size with different zeta-potential could be manipulated just by 5-ALA concentrations and pH values. CNA particles prepared at pH 7.4 and pH 9 of 5-ALA solutions with a concentration higher than 0.5 mg/ml showed a promising loading efficiency of up to 75% and an optimum average particle size of 100 nm. The zeta-potential for CNA was over 30 mV that kept the nano-particle stable without aggregation when stored in suspension solution. Fluorescence microscope examination showed that CNA could be engulfed by Caco-2 colon cancer cells but showed no evidence of being taken up by Escherichia coli. This result implies that CNA could exclude the influence of normal flora inside the gut and serves as an adequate tool for fluorescent endoscopic detection of colorectal cancer cells in vivo.
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“Conjugating 5-aminolevulinic acid to nanoparticles, including biocompatible gold (Au) and chitosan, was one of the solutions suggested to improve ALA delivery to the tumor [78,79].”
From Aminolevulinic Acid (ALA) as a Prodrug in Photodynamic Therapy of Cancer (2011) · cites this paper as [79]
Citations
Cites
Recent advances on chitosan-based micro- and nanoparticles in drug delivery
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Oral drug absorption enhancement by chitosan and its derivatives
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Research works printed but not linked: 1
- Mountney, 1994, Colorectal cancer, vol. 1
Cited by
Sorted by citations
Arias, L. S., Pessan, J. P., Vieira, A. P. M., de Lima, T. M. T., Delbem, A. C. B., & Monteiro, D. R. (2018) · Antibiotics · doi:10.3390/antibiotics7020046 · Cited by 749
Yang, S.-J., Lin, F.-H., Tsai, K.-C., Wei, M.-F., Tsai, H.-M., Wong, J.-M., & Shieh, M.-J. (2010) · Bioconjugate Chemistry · doi:10.1021/bc9004798 · Cited by 272
Self-citationAminolevulinic Acid (ALA) as a Prodrug in Photodynamic Therapy of Cancer
Wachowska, M., Muchowicz, A., Firczuk, M., Gabrysiak, M., Winiarska, M., Wańczyk, M., Bojarczuk, K., & Golab, J. (2011) · Molecules · doi:10.3390/molecules16054140 · Cited by 265
Banerjee, A., Pathak, S., Subramanium, V. D., G., D., Murugesan, R., & Verma, R. S. (2017) · Drug Discovery Today · doi:10.1016/j.drudis.2017.05.006 · Cited by 204
Chitosan-based nanoparticles as drug delivery systems: a review on two decades of research
Naskar, S., Kuotsu, K., & Sharma, S. (2018) · Journal of drug targeting · doi:10.1080/1061186x.2018.1512112 · Cited by 226
Metadata
- Authors
- Yang, S.-J., Shieh, M.-J., Lin, F.-H., Lou, P.-J., Peng, C.-L., Wei, M.-F., Yao, C.-J., Lai, P.-S., & Young, T.-H.
- Affiliations
- National Taiwan University (TW)
- Tao Yuan General Hospital (TW)
- National Chung Hsing University (TW)
- Corresponding author
- Shieh, Ming-Jium
- ORCID
- Venue
- Cancer Letters
- Publisher
- Elsevier BV
- Published
- 2009-01
- Volume
- 273
- Issue
- 2
- Pages
- 210-220
- ISSN
- 0304-3835 (ISSN-L)
- OpenAlex
- W2131829423
- Cited by
- 56
- References
- 27
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 1 · 2021: 8 · 2020: 4 · 2019: 3 · 2018: 4 · 2017: 7 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 8
- FWCI
- 2.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cancer Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Photodynamic Therapy Research Studies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- colorectal cancer, 5-aminolevulinic acid, chitosan nanoparticles, sodium tripolyphosphate, Caco-2 cells, drug loading efficiency, zeta potential
- MeSH terms
- Aminolevulinic Acid, Endoscopy, Escherichia coli, Humans, Hydrogen-Ion Concentration, Medical Oncology, Microscopy, Fluorescence, Particle Size, Polyphosphates, Colorectal Neoplasms, Photosensitizing Agents, Caco-2 Cells, Microscopy, Electron, Transmission, Chitosan, Nanoparticles
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