The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Colorectal cancer cell detection by 5-aminolaevulinic acid-loaded chitosan nano-particles
Abstract
Colorectal cancer is one of the leading causes of malignant death in Taiwan because it often remains undetected until later stages of the disease. In this study, we designed an oral form nano-particle to encapsulate 5-aminolaevulinic acid (5-ALA) to improve the detection of colorectal cancer cells in vivo. The nano-particle should escape from bacteria uptake in the gastrointestinal tract which seriously interferes the results of endoscopic observation. In this study, chitosan was mixed with sodium tripolyphosphate (STPP) and 5-ALA to prepare chitosan nano-particles (CN) and 5-ALA loaded chitosan nano-particles (CNA) by adding different pH values and concentrations of 5-ALA solution. The average particle size and zeta-potential of CN and CNA were measured by the Zetasizer-3000. The results revealed that particle size with different zeta-potential could be manipulated just by 5-ALA concentrations and pH values. CNA particles prepared at pH 7.4 and pH 9 of 5-ALA solutions with a concentration higher than 0.5 mg/ml showed a promising loading efficiency of up to 75% and an optimum average particle size of 100 nm. The zeta-potential for CNA was over 30 mV that kept the nano-particle stable without aggregation when stored in suspension solution. Fluorescence microscope examination showed that CNA could be engulfed by Caco-2 colon cancer cells but showed no evidence of being taken up by Escherichia coli. This result implies that CNA could exclude the influence of normal flora inside the gut and serves as an adequate tool for fluorescent endoscopic detection of colorectal cancer cells in vivo.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Conjugating 5-aminolevulinic acid to nanoparticles, including biocompatible gold (Au) and chitosan, was one of the solutions suggested to improve ALA delivery to the tumor [78,79].”
From Aminolevulinic Acid (ALA) as a Prodrug in Photodynamic Therapy of Cancer (2011) · cites this paper as [79]
Citations
Cites
Recent advances on chitosan-based micro- and nanoparticles in drug delivery
Agnihotri, S. A., Nadagouda, M. N., & Aminabhavi, T. M. (2004) · Journal of Controlled Release · doi:10.1016/j.jconrel.2004.08.010 · Cited by 2,627
Preparation and antibacterial activity of chitosan nanoparticles
Qi, L., Xu, Z., Jiang, X., Hu, C., & Zou, X. (2004) · Carbohydrate Research · doi:10.1016/j.carres.2004.09.007 · Cited by 1,808
Photodynamic therapy with endogenous protoporphyrin
Kennedy, J. C., Pottier, R. H., & Pross, D. C. (1990) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/1011-1344(90)85083-9 · Cited by 1,509
Gan, Q., Wang, T., Cochrane, C., & McCarron, P. A. (2005) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2005.06.001 · Cited by 908
Oral drug absorption enhancement by chitosan and its derivatives
Thanou, M., Verhoef, J. C., & Junginger, H. E. (2001) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(01)00231-9 · Cited by 705
Antimicrobial effect of chitooligosaccharides produced by bioreactor
Jeon, Y., Park, P., & Kim, S.-K. (2001) · Carbohydrate Polymers · doi:10.1016/s0144-8617(00)00200-9 · Cited by 679
Synthesis and characterization of chitosan–poly(acrylic acid) nanoparticles
Hu, Y., Jiang, X., Ding, Y., Ge, H., Yuan, Y., & Yang, C. (2002) · Biomaterials · doi:10.1016/s0142-9612(02)00071-6 · Cited by 513
Chitosan and its derivatives as intestinal absorption enhancers
Thanou, M., Verhoef, J. C., & Junginger, H. E. (2001) · Advanced Drug Delivery Reviews · doi:10.1016/s0169-409x(01)00180-6 · Cited by 437
Antibacterial characteristics and activity of acid-soluble chitosan
Chung, Y., & Chen, C. (2007) · Bioresource Technology · doi:10.1016/j.biortech.2007.06.044 · Cited by 406
Hu, B., Pan, C., Sun, Y., Hou, Z., Ye, H., Hu, B., & Zeng, X. (2008) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf801111c · Cited by 386
Research works printed but not linked: 1
- Mountney, 1994, Colorectal cancer, vol. 1
Cited by
Sorted by citations
Arias, L. S., Pessan, J. P., Vieira, A. P. M., de Lima, T. M. T., Delbem, A. C. B., & Monteiro, D. R. (2018) · Antibiotics · doi:10.3390/antibiotics7020046 · Cited by 749
Yang, S.-J., Lin, F.-H., Tsai, K.-C., Wei, M.-F., Tsai, H.-M., Wong, J.-M., & Shieh, M.-J. (2010) · Bioconjugate Chemistry · doi:10.1021/bc9004798 · Cited by 272
Self-citationAminolevulinic Acid (ALA) as a Prodrug in Photodynamic Therapy of Cancer
Wachowska, M., Muchowicz, A., Firczuk, M., Gabrysiak, M., Winiarska, M., Wańczyk, M., Bojarczuk, K., & Golab, J. (2011) · Molecules · doi:10.3390/molecules16054140 · Cited by 265
Banerjee, A., Pathak, S., Subramanium, V. D., G., D., Murugesan, R., & Verma, R. S. (2017) · Drug Discovery Today · doi:10.1016/j.drudis.2017.05.006 · Cited by 204
Chitosan-based nanoparticles as drug delivery systems: a review on two decades of research
Naskar, S., Kuotsu, K., & Sharma, S. (2018) · Journal of drug targeting · doi:10.1080/1061186x.2018.1512112 · Cited by 226
Drug Delivery Nanoparticles in Skin Cancers
Dianzani, C., Zara, G. P., Maina, G., Pettazzoni, P., Pizzimenti, S., Rossi, F., Gigliotti, C. L., Ciamporcero, E. S., Daga, M., & Barrera, G. (2014) · BioMed Research International · doi:10.1155/2014/895986 · Cited by 187
Magnetic chitosan nanoparticles as a drug delivery system for targeting photodynamic therapy
Sun, Y., Chen, Z., Yang, X., Huang, P., Zhou, X.-P., & Du, X. (2009) · Nanotechnology · doi:10.1088/0957-4484/20/13/135102 · Cited by 140
Reversal of doxorubicin-resistance by multifunctional nanoparticles in MCF-7/ADR cells
Shieh, M., Hsu, C., Huang, L.-Y., Chen, H., Huang, F., & Lai, P. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2011.03.017 · Cited by 122
Self-citationYang, S., Lin, F., Tsai, H.-M., Lin, C.-F., Chin, H.-C., Wong, J., & Shieh, M. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.11.039 · Cited by 117
Self-citationCancer nanotheranostics: A review of the role of conjugated ligands for overexpressed receptors
Ramzy, L., Nasr, M., Metwally, A. A., & Awad, G. A. S. (2017) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2017.04.005 · Cited by 107
Hodgkinson, N., Kruger, C. A., & Abrahamse, H. (2017) · Tumor Biology · doi:10.1177/1010428317734691 · Cited by 106
Nanosonotechnology: the next challenge in cancer sonodynamic therapy
Serpe, L., Foglietta, F., & Canaparo, R. (2012) · Nanotechnology Reviews · doi:10.1515/ntrev-2011-0009 · Cited by 87
Chitosan: a potential polymer for colon-specific drug delivery system
Gulbake, A., & Jain, S. K. (2012) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2012.682148 · Cited by 81
In vitro evaluation of 5-aminolevulinic acid (ALA) loaded PLGA nanoparticles
Shi, L., Wang, X., Zhao, F., Luan, H., Tu, Q., Huang, Z. W., Wang, H., & Wang, H. (2013) · International Journal of Nanomedicine · doi:10.2147/ijn.s45821 · Cited by 81
Photodynamic therapy in colorectal cancer treatment—The state of the art in preclinical research
Kawczyk‐Krupka, A., Bugaj, A. M., Latos, W., Zaremba, K., Wawrzyniec, K., Kucharzewski, M., & Sieroń, A. (2015) · Photodiagnosis and Photodynamic Therapy · doi:10.1016/j.pdpdt.2015.07.175 · Cited by 71
Gorgizadeh, M., Azarpira, N., Lotfi, M., Daneshvar, F., Salehi, F., & Sattarahmady, N. (2019) · Photodiagnosis and Photodynamic Therapy · doi:10.1016/j.pdpdt.2019.05.023 · Cited by 58
Tao, L., Zhang, K., Sun, Y., Jin, B., Zhang, Z., & Yang, K. (2012) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2012.02.044 · Cited by 50
Prateeksha, Sharma, V. K., Liu, X., Oyarzún, D. A., Abdel-Azeem, A. M., Atanasov, A. G., Hesham, A. E., Barik, S. K., Gupta, V. K., & Singh, B. N. (2021) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2021.05.021 · Cited by 47
Wang, J., Wang, K., Liang, J., Jin, J., Wang, X., & Yan, S. (2021) · Photodiagnosis and Photodynamic Therapy · doi:10.1016/j.pdpdt.2021.102581 · Cited by 41
Chitosan-Based Nanoparticles in Cancer Therapy
Lakshmanan, V., Snima, K. S., Bumgardner, J. D., Nair, S. V., & Jayakumar, R. (2011) · Advances in polymer science · doi:10.1007/12_2011_132 · Cited by 41
Dasari, S. R., Yedjou, C. G., Brodell, R. T., Cruse, A. R., & Tchounwou, P. B. (2020) · Nanotechnology Reviews · doi:10.1515/ntrev-2020-0117 · Cited by 40
Chen, J., Li, X., Liu, Y., Su, T., Lin, C., Shao, L., Li, L., Li, W., Niu, G., Yu, J., et al. (2021) · Microbial Biotechnology · doi:10.1111/1751-7915.13894 · Cited by 39
Photosensitizer-loaded dendrimer-modified multi-walled carbon nanotubes for photodynamic therapy
Huang, P., Lin, J., Yang, D., Zhang, C., Li, Z., & Cui, D. (2011) · Journal of Controlled Release · doi:10.1016/j.jconrel.2011.08.105 · Cited by 37
Photodynamic Detection of Oral Cancers with High-Performance Chitosan-Based Nanoparticles
Yang, S., Lin, C.-F., Kuo, M., & Tan, C. (2013) · Biomacromolecules · doi:10.1021/bm400820s · Cited by 33
Self-citationAutofluorescence Imaging and Spectroscopy of Human Lung Cancer
Wang, M., Long, F., Tang, F., Jing, Y., Wang, X., Yao, L., Ma, J., Fei, Y., Chen, L., Wang, G., et al. (2016) · Applied Sciences · doi:10.3390/app7010032 · Cited by 29
Application of different nanoparticles in the diagnosis of colorectal cancer
Ghorbani, F., Kokhaei, P., Ghorbani, M., & Eslami, M. (2020) · Gene Reports · doi:10.1016/j.genrep.2020.100896 · Cited by 28
Therapeutic nanosystems for oncology nanomedicine
Gonçalves, A. S., Macedo, A. S., & Souto, E. B. (2012) · Clinical & Translational Oncology · doi:10.1007/s12094-012-0912-1 · Cited by 28
Sriram, K., Maheswari, P. U., Ezhilarasu, A., Begum, K. M. M. S., & Arthanareeswaran, G. (2017) · Asia-Pacific Journal of Chemical Engineering · doi:10.1002/apj.2124 · Cited by 26
Nanochitosan: Commemorating the Metamorphosis of an ExoSkeletal Waste to a Versatile Nutraceutical
Sivanesan, I., Muthu, M., Gopal, J., Hasan, N., Ali, S. K., Shin, J., & Oh, J. (2021) · Nanomaterials · doi:10.3390/nano11030821 · Cited by 27
Kavianinia, I., Plieger, P. G., Kandile, N. G., & Harding, D. R. K. (2015) · Materials Today Communications · doi:10.1016/j.mtcomm.2015.03.002 · Cited by 25
Kim, C. H. C. H., Chung, C., Lee, H. M. H. M., Kim, D. H. D. H., Kwak, T. W., Jeong, Y., & Kang, D. H. (2013) · International Journal of Nanomedicine · doi:10.2147/ijn.s44394 · Cited by 21
Oral colon cancer targeting by chitosan nanocomposites
Bose, A., & Wong, T. W. (2018) · Applications of Nanocomposite Materials in Drug Delivery · doi:10.1016/b978-0-12-813741-3.00018-2 · Cited by 12
Kaczorowska, A., Malinga-Drozd, M., Kałas, W., Kopaczyńska, M., Wołowiec, S., & Borowska, K. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22041982 · Cited by 18
Rata-Aguilar, A., Sánchez‐Moreno, P., Jódar‐Reyes, A. B., Martín-Rodríguez, A., Boulaiz, H., Marchal, J. A., Peula-García, J. M., & Ortega-Vinuesa, J. L. (2012) · Journal of Bioactive and Compatible Polymers · doi:10.1177/0883911512447492 · Cited by 17
Martino, A. D., Pavelková, A., Постников, П. С., & Sedlařík, V. (2017) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2017.08.010 · Cited by 17
Roy, H., Nayak, B. S., & Nandi, S. (2020) · Current Pharmaceutical Design · doi:10.2174/1381612826666200203121241 · Cited by 18
Nanomedicine and Cancer Therapies
Khoobchandani, M., Ganesh, N., Valgimigli, L., & M., S. M. (2012) · Apple Academic Press eBooks · doi:10.1201/b13120 · Cited by 17
Improving chemotherapy drug delivery by nanoprecision tools
Salahuddin, N. A., & Galal, A. (2017) · Elsevier eBooks · doi:10.1016/b978-0-323-46144-3.00004-0 · Cited by 14
Influence of silver nanoparticles on settling of suspended sediments
Wang, Y., Sun, Y., Gong, S., Cai, Z., & Fu, J. (2019) · Journal of Molecular Liquids · doi:10.1016/j.molliq.2019.112135 · Cited by 10
Targeted Photodynamic Therapy as Potential Treatment Modality for the Eradication of Colon Cancer
Kruger, C. A., & Abrahamse, H. (2019) · IntechOpen eBooks · doi:10.5772/intechopen.84760 · Cited by 10
Re, F., Pelucchi, S., Pettini, F., Prantera, A., Troisi, F., Mahmoud, R. M. A., Giovannoni, R., & Barisani, D. (2025) · Nanomedicine · doi:10.1080/17435889.2025.2520157 · Cited by 9
M'zyène, F., Moulay, S., Bal, K., & Fatiha, M. (2018) · Journal of Inclusion Phenomena and Macrocyclic Chemistry · doi:10.1007/s10847-018-0842-9 · Cited by 5
Suryani, A. I., Wathoni, N., Muchtaridi, M., & Joni, I. M. (2021) · International Journal of Applied Pharmaceutics · doi:10.22159/ijap.2021.v13s4.43818 · Cited by 5
Chitosan Nanoparticles: A Potential Biomedical Device
Jafernik, K., Malinowska, M. A., Bialik-Wąs, K., Sharafan, M., Ekiert, H., Sikora, E. S., & Szopa, A. (2023) · Biological and medical physics, biomedical engineering · doi:10.1007/978-981-19-9646-7_6 · Cited by 5
Shah, D., & Londhe, V. Y. (2011) · Therapeutic Delivery · doi:10.4155/tde.10.102 · Cited by 5
de Andrade, L. R., Primo, F. L., Silva, D., Tedesco, A. C., & Lacava, Z. G. M. (2017) · Photodiagnosis and Photodynamic Therapy · doi:10.1016/j.pdpdt.2017.08.011 · Cited by 5
Zhao, N., Chen, H., Cai, S., Li, Q., Wang, J., Wang, W., & Lv, C. (2025) · Industrial Crops and Products · doi:10.1016/j.indcrop.2025.121608 · Cited by 4
Targeting colorectal cancer via nanodrug delivery systems
Zhong, J., Ma, X., & Liang, X. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-819937-4.00011-x · Cited by 2
Cerize, N. N. P. (2012) · doi:10.11606/t.60.2012.tde-03072012-140751 · Cited by 2
Chitosan nanoparticles for gene delivery in cancer therapy and disease diagnosis
Arya, G., & Sharma, T. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-15401-0.00002-6 · Cited by 1
Jin, K., Zhang, Y., Huang, Y. B., Yu, L., Zhong, Y., Niu, L., & Wu, Y. (2025) · Advanced Healthcare Materials · doi:10.1002/adhm.202504077 · Cited by 1
Alginate Particulate Gels for Oral Drug Delivery
Sosnik, A. (2021) · Royal Society of Chemistry eBooks · doi:10.1039/9781839161124-00155 · Cited by 1
Steady State Fluorescence Spectroscopy for Medical Diagnosis
Mahadevan-Jansen, A. M., & Gebhart, S. C. (2010) · Springer eBooks · doi:10.1007/978-90-481-8831-4_20
Kozak, R. (2014) · RIUnB Institutional Repository (University of Brasília) · doi:10.26512/2014.12.d.17766
NANO-BASED THERAPY FOR TREATMENT OF SKIN CARCINOMA
Jena, G. K., & Patra, C. N. (2021) · INDIAN DRUGS · doi:10.53879/id.58.11.12216
Metadata
- Authors
- Yang, S.-J., Shieh, M.-J., Lin, F.-H., Lou, P.-J., Peng, C.-L., Wei, M.-F., Yao, C.-J., Lai, P.-S., & Young, T.-H.
- Affiliations
- National Taiwan University (TW)
- Tao Yuan General Hospital (TW)
- National Chung Hsing University (TW)
- Corresponding author
- Shieh, Ming-Jium
- ORCID
- Venue
- Cancer Letters
- Publisher
- Elsevier BV
- Published
- 2009-01
- Volume
- 273
- Issue
- 2
- Pages
- 210-220
- ISSN
- 0304-3835 (ISSN-L)
- OpenAlex
- W2131829423
- Cited by
- 56
- References
- 27
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 1 · 2021: 8 · 2020: 4 · 2019: 3 · 2018: 4 · 2017: 7 · 2016: 1 · 2015: 2 · 2014: 2 · 2013: 3 · 2012: 8
- FWCI
- 2.76
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cancer Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Photodynamic Therapy Research Studies
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- colorectal cancer, 5-aminolevulinic acid, chitosan nanoparticles, sodium tripolyphosphate, Caco-2 cells, drug loading efficiency, zeta potential
- MeSH terms
- Aminolevulinic Acid, Endoscopy, Escherichia coli, Humans, Hydrogen-Ion Concentration, Medical Oncology, Microscopy, Fluorescence, Particle Size, Polyphosphates, Colorectal Neoplasms, Photosensitizing Agents, Caco-2 Cells, Microscopy, Electron, Transmission, Chitosan, Nanoparticles
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
