Cited by 30Cell death mechanisms and regulationAutophagy in Disease and TherapyCancer-related Molecular Pathways

Identification of ADP-ribosylation factor 4 as a suppressor of N-(4-hydroxyphenyl)retinamide-induced cell death

Woo, Im Sun, Eun, So Young, Jang, Han-Su, Kang, Eun Sil, Kim, Gil Hyeong, Kim, Hye Jung, Lee, Jae Heun, Chang, Ki Churl +3 more·2009·Cancer Letters

Abstract

Yeast-based functional screening for inhibitors of Bcl-2-associated X protein (Bax)-induced cell death in yeast identified ADP-ribosylation factor 4 (ARF4) as a novel anti-apoptotic gene in human glioblastoma-derived U373MG cells. Yeast or U373MG cells that overexpressed ARF4 exhibited reduced reactive oxygen species (ROS) generation in response to Bax or N-(4-hydroxyphenyl)retinamide (4-HPR), respectively, which suggests that ROS play a role in the inhibition of cell death by ARF4. The 4-HPR-mediated phosphorylation of c-JUN N-terminal kinase, p38, and extracellular signal-regulated kinase was markedly suppressed in U373MG cells that stably expressed ARF4. Stable ARF4 transfectants were also refractory to 4-HPR-induced mitochondrial translocation of Bax, release of mitochondrial cytochrome c, and activation of caspase-3. Our results suggest that ARF4 participates in the regulation of glioblastoma apoptosis through the inhibition of stress-mediated apoptotic signals.

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Crossref · 10.1016/j.canlet.2008.10.031

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Authors
Woo, I. S., Eun, S. Y., Jang, H.-S., Kang, E. S., Kim, G. H., Kim, H. J., Lee, J. H., Chang, K. C., Kim, J.-H., Han, C. W., & Seo, H. G.
Affiliations
  • Gyeongsang National University (KR)
  • Konkuk University (KR)
  • Dongguk University (KR)
Venue
Cancer Letters
Publisher
Elsevier BV
Published
2009-04
Volume
276
Issue
1
Pages
53-60
ISSN
0304-3835 (ISSN-L)
OpenAlex
W2005061126
Cited by
30
References
35
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 5 · 2020: 4 · 2017: 1 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 2
FWCI
0.82
Type
journal article
Language
en
Open access
closed
Locations
  • Cancer Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
Keywords
ARF4, N-(4-hydroxyphenyl)retinamide, apoptosis, Bax, reactive oxygen species, c-Jun N-terminal kinase, p38 MAPK, ERK, cytochrome c release, Bax translocation, caspase-3, glioblastoma, U373 cells, anti-apoptotic genes, stress-induced apoptosis
MeSH terms
Antineoplastic Agents, Flow Cytometry, Glioblastoma, Humans, Transfection, Blotting, Western, Gene Library, Gene Expression, Mutagenesis, Site-Directed, Apoptosis, Fenretinide, Reactive Oxygen Species, Oxidative Stress, ADP-Ribosylation Factors, Two-Hybrid System Techniques, Cell Line, Tumor, JNK Mitogen-Activated Protein Kinases, Extracellular Signal-Regulated MAP Kinases, p38 Mitogen-Activated Protein Kinases, bcl-2-Associated X Protein

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