Method for preparation of planar alginate hydrogels by external gelling using an aerosol of gelling solution
Abstract
Preparation of planar alginate hydrogels by external gelling requires slow rate of exposure of alginate solution to gelling ions to control gelling process and hydrogel properties. We tackled this issue by exposing solution of sodium alginate to solution of CaCl2 applied as aerosol at exposure rate of 7.5 mg cm(-2) s(-1). Gelling conditions varied with respect to concentrations of sodium alginate (1-3 wt.%) and CaCl2 (0.5-4 wt.%), exposure time (2.5-40 min), the 2nd gelling step in the presence of barium ions, and the storage step. Dimensional stability and Young's modulus values were the principal determined quantities to examine the correlation between hydrogel properties and gelling protocol. The content of calcium ions in hydrogel after gelling by CaCl2 aerosol reveals that the maximum binding capacity of calcium ions by alginate chains was reached. Obtained data suggest that an unusual gelling mechanism related to exposure of sodium alginate to aerosol of gelling solution does not need to be considered since the properties of planar alginate hydrogels follow the trends relevant to general knowledge about alginate hydrogels.
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Size and shape, rather than stiffness, play a critical role in biocompatibility, based on evaluation of materials with stiffness ranging from soft alginate hydrogels (<100 kPa) to hard stainless steel (100s of GPa).
“Notably, our evaluation of a spectrum of materials with a range of mechanical stiffness properties varying from soft (Alginate hydrogels, < 100 kPa)34 to hard (Stainless steel, 100s of GPa)35 demonstrates that size and shape rather than stiffness plays a critical role in biocompatibility.”
From Size- and shape-dependent foreign body immune response to materials implanted in rodents and non-human primates (2015) · cites this paper as [34]
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Self-citation
- Authors
- Papajová, E., Bujdoš, M., Chorvát, D., Stach, M., & Lacík, I.
- Affiliations
- Polymer Institute of the Slovak Academy of Sciences (SK)
- Comenius University Bratislava (SK)
- ORCID
- Bujdoš, Marek
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2012-09
- Volume
- 90
- Issue
- 1
- Pages
- 472-482
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W2071860020
- Cited by
- 33
- References
- 51
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 2 · 2022: 1 · 2020: 1 · 2019: 4 · 2018: 3 · 2017: 1 · 2016: 5 · 2015: 4 · 2014: 2 · 2013: 1
- FWCI
- 1.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Polymers (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Agentúra na Podporu Výskumu a Vývoja (APVV-0302-10, APVV-0486-10)
- European Regional Development Fund
- Vedecká Grantová Agentúra MŠVVaŠ SR a SAV (2/0163/12)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Keywords
- alginate hydrogel, sodium alginate, barium ions, dimensional stability, Young's modulus, calcium binding capacity, gelling mechanism, hydrogel synthesis
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