Enhancement and modeling of enzymatic hydrolysis on cellulose from agave bagasse hydrothermally pretreated in a horizontal bioreactor
Open accessAbstract
One of the major challenges in biofuels production from lignocellulosic biomass is the generation of high glucose titers from cellulose in the enzymatic hydrolysis stage of pretreated biomass to guarantee a cost-effective process. Therefore, the enzymatic saccharification on cellulose at high solid loading is an alternative. In this work, the agave bagasse was hydrothermally pretreated and optimized at 194 °C/30 min, obtaining a pretreated solid rich in cellulose content (>46.46%), and subjected to enzymatic hydrolysis at high solid levels. A horizontal bioreactor was designed for enzyme saccharification at high solid loadings [25% (w/v)]. The bioreactor improved mixing efficiency, with cellulose conversions up to 98% (195.6 g/L at 72 h). Moreover, mathematical modeling of cellulase deactivation demonstrated that cellulases lose most of their initial activity in the first hours of the reaction. Also, cellulose was characterized by X-ray diffraction, and the pretreated solids were visualized using scanning electron microscopy.
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The differences in glucose yields between this study and Pino et al. are attributed to the type of biomass used.
“The differences are attributed to the type of biomass used”
From High-pressure technology for Sargassum spp biomass pretreatment and fractionation in the third generation of bioethanol production (2021) · cites this paper as Pino et al. (2019)
Section 3.3.2.1 (page unavailable) says final sugar concentration depends on the raw material’s initial glucan content and compares that content across biomasses. This supports biomass composition as a possible explanation, but the paper does not assess the citing study’s glucose yields; it also distinguishes glucose concentration from glucose yield. (LLM, conf=0.82)
Experimental — accuracy not yet measuredThe glucan content in the Sargassum used in this study was 32.33%, compared to 46.46% in pretreated lignocellulosic biomass.
“where the glucan in pretreated lignocellulosic biomass was 46.46 %, compared to 32.33 % in the Sargassum used in this study.”
From High-pressure technology for Sargassum spp biomass pretreatment and fractionation in the third generation of bioethanol production (2021) · cites this paper as Pino et al. (2019)
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Research works printed but not linked: 1
- Ruiz, H. A., Thomsen, M. H., & Trajano, H. L. (2017). Hydrothermal processing in bior- efineries. Cham, Switzerland: Springer International Publishing.
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Aparicio, E., Rodríguez-Jasso, R. M., Pinales-Márquez, C. D., Loredo-Treviño, A., Robledo-Olivo, A., Aguilar, C. N., Kostas, E. T., & Ruiz, H. A. (2021) · Bioresource Technology · doi:10.1016/j.biortech.2021.124935 · Cited by 106 · see citing works
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- Authors
- Pino, M. S., Rodríguez-Jasso, R. M., Michelin, M., & Ruiz, H. A.
- Affiliations
- Universidad Autónoma de Coahuila (MX)
- University of Minho (PT)
- ORCID
- Michelin, Michele
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2019-05
- Volume
- 211
- Pages
- 349-359
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W2913497255
- Cited by
- 99
- References
- 39
- Citations by year
- 2026: 5 · 2025: 9 · 2024: 17 · 2023: 13 · 2022: 21 · 2021: 16 · 2020: 14 · 2019: 4
- FWCI
- 4.85
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Carbohydrate Polymers (publishedVersion)
- PubMed (publishedVersion)
- RepositóriUM (Universidade do Minho) (submittedVersion, other-oa)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by-nc-nd)
- Funders
- Consejo Nacional de Ciencia y Tecnología (254808, 2015-01)
- Fundação para a Ciência e a Tecnologia (SFRH/BPD/100786/2014)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Physical Sciences › Engineering › Biomedical Engineering › Catalysis for Biomass Conversion
- Keywords
- agave bagasse, hydrothermal pretreatment, enzymatic hydrolysis, cellulose hydrolysis, high solid loading, cellulose conversion, lignocellulosic biomass, biofuels, glucose yield
- MeSH terms
- Cellulase, Cellulose, Hot Temperature, Hydrolysis, Models, Theoretical, Bioreactors, Agave
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