Dextran-based scaffolds for in-situ hydrogelation: Use for next generation of bioartificial cardiac tissues
Abstract
In pursuit of a chemically-defined matrix for in vitro cardiac tissue generation, we present dextran (Dex)-derived hydrogels as matrices suitable for bioartificial cardiac tissues (BCT). The dextran hydrogels were generated in situ by using hydrazone formation as the crosslinking reaction. Material properties were flexibly adjusted, by varying the degrees of derivatization and the molecular weight of dextran used. Furthermore, to modulate dextran's bioactivity, cyclic pentapeptide RGD was coupled to its backbone. BCTs were generated by using a blend of modified dextran and human collagen (hColI) in combination with induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and fibroblasts. These hColI + Dex blends with or without RGD supported tissue formation and functional maturation of CMs. Contraction forces (hColI + Dex-RGD: 0.27 ± 0.02 mN; hColI + Dex: 0.26 ± 0.01 mN) and frequencies were comparable to published constructs. Thus, we could demonstrate that, independent of the presence of RGD, our covalently linked dextran hydrogels are a promising matrix for building cardiac grafts.
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The issue of bulk deterioration and a lack of local flexibility is raised by typically irreversible chemical crosslinking.
“The issue of bulk deterioration and a lack of local flexibility is raised by typically irreversible chemical crosslinking [117].”
From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [117]
Cites
Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., et al. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0506580102 · Cited by 53,625 · see citing works
Chemically defined generation of human cardiomyocytes
Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., Lan, F., Diecke, S., Huber, B., Mordwinkin, N. M., et al. (2014) · Nature Methods · doi:10.1038/nmeth.2999 · Cited by 1,606 · see citing works
Extracellular Matrix Assembly: A Multiscale Deconstruction
Mouw, J. K., Ou, G., & Weaver, V. M. (2014) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3902 · Cited by 1,534 · see citing works
Weng, Z., Kong, C.-W., Ren, L., Karakikes, I., Geng, L., He, J., Chow, M. Z. Y., Mok, C. F., Chan, H. Y. S., Webb, S. E., et al. (2014) · Stem Cells and Development · doi:10.1089/scd.2013.0509 · Cited by 109 · see citing works
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Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review
Vach Agocsova, S., Culenova, M., Birova, I., Omanikova, L., Moncmanova, B., Danisovic, L., Ziaran, S., Bakos, D., & Alexy, P. (2023) · Materials · doi:10.3390/ma16124267 · Cited by 98 · see citing works
- Authors
- Banerjee, S., Szepes, M., Dibbert, N., Rios-Camacho, J.-C., Kirschning, A., Gruh, I., & Dräger, G.
- Affiliations
- Leibniz University Hannover (DE)
- Medizinische Hochschule Hannover (DE)
- Corresponding author
- Dräger, Gerald
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2021-06
- Volume
- 262
- Pages
- 117924
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W3138352778
- Cited by
- 25
- References
- 4
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 5 · 2023: 5 · 2022: 5
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Carbohydrate Polymers (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Lower Saxony State Ministry of Science and Culture
- DFG
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- dextran hydrogels, hydrazone crosslinking, RGD peptide, iPSC-derived cardiomyocytes, fibroblasts, cardiac tissue engineering, cardiomyocyte contraction
- MeSH terms
- Collagen, Cross-Linking Reagents, Dextrans, Fibroblasts, Humans, Hydrazones, Myocardium, Hydrogels, Tissue Engineering, Myocytes, Cardiac, Tissue Scaffolds, Induced Pluripotent Stem Cells
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