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Cited by 185Cardiac electrophysiology and arrhythmiasIon channel regulation and functionCardiac Arrhythmias and Treatments

Apico-basal inhomogeneity in distribution of ion channels in canine and human ventricular myocardium

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SZENTADRASSY, N, BANYASZ, T, BIRO, T, SZABO, G, TOTH, B, MAGYAR, J, LAZAR, J, VARRO, A +2 more·2005·Cardiovascular Research
DOI 10.1016/j.cardiores.2004.11.022
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Abstract

OBJECTIVES: The aim of the present study was to compare the apico-basal distribution of ion currents and the underlying ion channel proteins in canine and human ventricular myocardium. METHODS: Ion currents and action potentials were recorded in canine cardiomyocytes, isolated from both apical and basal regions of the heart, using whole-cell voltage clamp techniques. Density of channel proteins in canine and human ventricular myocardium was determined by Western blotting. RESULTS: Action potential duration was shorter and the magnitude of phase-1 repolarization was significantly higher in apical than basal canine myocytes. No differences were observed in other parameters of the action potential or cell capacitance. Amplitude of the transient outward K(+) current (29.6+/-5.7 versus 16.5+/-4.4 pA/pF at +65 mV) and the slow component of the delayed rectifier K(+) current (5.61+/-0.43 versus 2.14+/-0.18 pA/pF at +50 mV) were significantly larger in apical than in basal myocytes. Densities of the inward rectifier K(+) current, rapid delayed rectifier K(+) current, and L-type Ca(2+) current were similar in myocytes of apical and basal origin. Apico-basal differences were found in the expression of only those channel proteins which are involved in mediation of the transient outward K(+) current and the slow delayed rectifier K(+) current: expression of Kv1.4, KChIP2, KvLQT1 and MinK was significantly higher in apical than in basal myocardium in both canine and human hearts. CONCLUSIONS: The results suggest that marked apico-basal electrical inhomogeneity exists in the canine-and probably in the human-ventricular myocardium, which may result in increased dispersion, and therefore, cannot be ignored when interpreting ECG recordings, pathological alterations, or drug effects.

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  • Expression patterns of various ion channels, both apico-basally and transmurally, are very similar between canine and human with some exceptions.

    “expression patterns of various ion channels both apico-basally (Szentadrassy, et al., 2005) and transmurally (Szabo, et al., 2005; Zicha, et al., 2004) with some exceptions, are very similar between these two species.”

    From Small and large animal models in cardiac contraction research: advantages and disadvantages (2014) · cites this paper as Szentadrassy, et al., 2005

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Authors
SZENTADRASSY, N., BANYASZ, T., BIRO, T., SZABO, G., TOTH, B., MAGYAR, J., LAZAR, J., VARRO, A., KOVACS, L., & NANASI, P.
Affiliations
University of Debrecen (HU)
ORCID
Venue
Cardiovascular Research
Publisher
Oxford University Press (OUP)
Published
2005-03-01
Volume
65
Issue
4
Pages
851-860
ISSN
0008-6363 (ISSN-L)
DOI
10.1016/j.cardiores.2004.11.022
OpenAlex
W2078928824
Cited by
185
References
27
Citations by year
2026: 5 · 2025: 5 · 2024: 5 · 2023: 2 · 2022: 8 · 2021: 14 · 2020: 10 · 2019: 4 · 2018: 5 · 2017: 9 · 2016: 6 · 2015: 9 · 2014: 12 · 2013: 11 · 2012: 12
FWCI
3.48
Type
journal article
Language
en
Open access
Locations
  • Cardiovascular Research (publishedVersion)
  • PubMed (publishedVersion)
  • University of Debrecen Electronic Archive (University of Debrecen) (submittedVersion, other-oa)
Funders
Hungarian Scientific Research Fund
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Arrhythmias and Treatments
Keywords
ventricular myocardium, ion channels, ionic currents, action potential duration, transient outward potassium current, slow delayed rectifier potassium current, Kv1.4, KChIP2, KCNQ1, mink, human ventricular myocytes, dispersion of repolarization, ECG interpretation
MeSH terms
Action Potentials, Animals, Dogs, Female, Heart Ventricles, Humans, Ion Channels, Male, Membrane Potentials, Myocardium, Blotting, Western, Ion Pumps, Patch-Clamp Techniques, Calcium Channels, L-Type, Potassium Channels, Voltage-Gated, Potassium Channels, Inwardly Rectifying, Myocytes, Cardiac, Delayed Rectifier Potassium Channels

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