The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Tests for the mechanism of starch biosynthesis: de novo synthesis or an amylogenin primer synthesis
Abstract
Studies in 1940 on potato phosphorylase reaction with starch found that d-glucopyranose from α-d-glucopyranosyl-1-phosphate was added to the nonreducing-ends of starch chains. This led to the hypothesis that the biosynthesis of starch required a preformed primer. Later it was found that phosphorylase was exclusively a degradative enzyme in vivo and that starch-synthase was the enzyme that reacted with ADPGlc to biosynthesize starch. Amylogenin, a putative self-glycosylated protein, was postulated to be the primer, although it was never demonstrated or found. In the present study, three reactions were performed in sequence with a highly purified potato starch-synthase to determine whether an amylogenin primer was present and required or whether the biosynthesis was de novo. Reaction 1 was performed by adding 2.0mM ADPGlc to synthesize the putative primer to a possible amylogenin in the preparation; in Reaction 2, 10mM ADP-[(14)C]Glc was added; and in Reaction 3, 10mM nonlabeled ADPGlc was added. After the isolation, reduction, and acid hydrolysis of the products of Reactions 2 and 3, (14)C-d-glucitol was obtained from Reaction 2 and was decreased by Reaction 3. The formation of (14)C-d-glucitol and its decrease showed that an amylogenin, protein primer was not involved in starch biosynthesis and the synthesis is de novo by the addition of d-glucose to the reducing-ends of growing starch chains.
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“In contrast, the mechanism of potato starch biosynthesis appeared to involve de novo synthesis, not an amylogenin primer (568).”
From Biological roles of glycans (2017) · cites this paper as [568]
Citations
Cites
BIOSYNTHESIS OF GLYCOGEN FROM URIDINE DIPHOSPHATE GLUCOSE1
Leloir, L. F., & Cardini, C. E. (1957) · Journal of the American Chemical Society · doi:10.1021/ja01580a061 · Cited by 413
Biochemical Techniques: Theory and Practice
Robyt, J. F., & White, B. J. (1987) · Medical Entomology and Zoology · Cited by 355
Self-citationAdenosine diphosphate glucose and starch synthesis
Recondo, E., & Leloir, L. F. (1961) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(61)90389-8 · Cited by 249
The reversible formation of starch from glucose-1- phosphate catalysed by potato phosphorylase
Hanes, C. S. (1940) · Proceedings of the Royal Society B Biological Sciences · doi:10.1098/rspb.1940.0035 · Cited by 196
A Precursor of Glycogen Biosynthesis: α‐1,4‐Glucan‐Protein
Krisman, C. R., & Barengo, R. (1975) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1975.tb03979.x · Cited by 133
Cheng, C., Mu, J., Farkas, I., Huang, D., Goebl, M. G., & Roach, P. J. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.12.6632 · Cited by 110
Glycogenin: the primer for mammalian and yeast glycogen synthesis
Lomako, J. (2004) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2004.03.017 · Cited by 95
β‐Glucosylarginine: a new glucose‐protein bond in a self‐glucosylating protein from sweet corn
Singh, D. G., Lomako, J., Lomako, W. M., Whelan, W. J., Meyer, H. E., Serwe, M., & Metzger, J. W. (1995) · FEBS Letters · doi:10.1016/0014-5793(95)01247-6 · Cited by 90
THE ACTIVATING EFFECT OF GLYCOGEN ON THE ENZYMATIC SYNTHESIS OF GLYCOGEN FROM GLUCOSE-1-PHOSPHATE
Cori, G. T., & Cori, C. F. (1939) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)73512-6 · Cited by 76
Glucosylation Activity and Complex Formation of Two Classes of Reversibly Glycosylated Polypeptides
Langeveld, S. M. J., Vennik, M., Kottenhagen, M. J., van Wijk, R., Buijk, A., Kijne, J. W., & de Pater, S. (2002) · PLANT PHYSIOLOGY · doi:10.1104/pp.010720 · Cited by 65
Cited by
Sorted by citations
Varki, A. (2017) · Glycobiology · doi:10.1093/glycob/cww086 · Cited by 2,635
“In contrast, the mechanism of potato starch biosynthesis appeared to involve de novo synthesis, not an amylogenin primer (568).”
Background
Metadata
- Authors
- Mukerjea, R., & Robyt, J. F.
- Affiliations
- Iowa State University (US)
- Venue
- Carbohydrate Research
- Publisher
- Elsevier BV
- Published
- 2013-05
- Volume
- 372
- Pages
- 55-59
- ISSN
- 0008-6215 (ISSN-L)
- OpenAlex
- W1995590464
- Cited by
- 4
- References
- 26
- Citations by year
- 2025: 1 · 2021: 1 · 2016: 1 · 2013: 1
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Glycogen Storage Diseases and Myoclonus
- Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- starch biosynthesis, ADP-glucose, starch phosphorylase, potato
- MeSH terms
- Glucose, Glycoproteins, Hydrolysis, Plant Proteins, Solanum tuberosum, Starch, Starch Synthase, Intramolecular Transferases
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Cite CoChat for the checks on this page, and the paper for its own findings.
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- The paper
- Mukerjea, R., & Robyt, J. F. (2013). Tests for the mechanism of starch biosynthesis: de novo synthesis or an amylogenin primer synthesis. Carbohydrate Research. https://doi.org/10.1016/j.carres.2013.01.020
