The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Optimizing non-natural protein function with directed evolution
Open accessAbstract
Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement these approaches: recent work combining unnatural amino acid mutagenesis and phage selection has created useful proteins of novel composition. And weak initial activity in a computationally designed enzyme has been improved by iterative rounds of mutagenesis and screening. A marriage of ingenuity and evolution will expand the scope of protein function well beyond Mother Nature’s designs.
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Cites
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Cited by
Kawecki, T. J., Lenski, R. E., Ebert, D., Hollis, B., Olivieri, I., & Whitlock, M. C. (2012) · Trends in Ecology & Evolution · doi:10.1016/j.tree.2012.06.001 · Cited by 807
Computational design of ligand-binding proteins with high affinity and selectivity
Tinberg, C. E., Khare, S. D., Dou, J., Doyle, L., Nelson, J. W., Schena, A., Jankowski, W., Kalodimos, C. G., Johnsson, K., Stoddard, B. L., et al. (2013) · Nature · doi:10.1038/nature12443 · Cited by 431
Porebski, B. T., & Buckle, A. M. (2016) · Protein Engineering Design and Selection · doi:10.1093/protein/gzw015 · Cited by 215
Rational Engineering of Enzyme Allosteric Regulation through Sequence Evolution Analysis
Yang, J.-S., Seo, S. W., Jang, S., Jung, G. Y., & Kim, S. (2012) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1002612 · Cited by 87
Platero-Rochart, D., Krivobokova, T., Gastegger, M., Reibnegger, G., & Sánchez-Murcia, P. A. (2023) · Journal of Chemical Information and Modeling · doi:10.1021/acs.jcim.3c00772 · Cited by 15
Metadata
- Authors
- Brustad, E. M., & Arnold, F. H.
- Venue
- Current Opinion in Chemical Biology
- Publisher
- Elsevier BV
- Published
- 2011-04
- Volume
- 15
- Issue
- 2
- Pages
- 201-210
- ISSN
- 1367-5931 (ISSN-L)
- OpenAlex
- W2077933693
- Cited by
- 255
- References
- 40
- FWCI
- 1.22
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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