The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher
Open accessAbstract
SpyTag is a short peptide that forms an isopeptide bond upon encountering its protein partner SpyCatcher. This covalent peptide interaction is a simple and powerful tool for bioconjugation and extending what protein architectures are accessible. Here we review the origin and mechanism of SpyTag/SpyCatcher, focusing on recent innovative applications. Ligation of targeting-antibody with antigen provided a simple route to vaccine generation. SpyRings, from head-to-tail cyclisation, gave major enhancements in enzyme resilience. Linking multiple SpyCatchers gave dendrimers for T-cell activation or Spy networks forming hydrogels for stem cell culture. Synthetic biology applications include integrating amyloid biomaterials with living bacteria, for irreversible derivatisation of biofilms with enzymes or nanoparticles. We also discuss further opportunities to apply and enhance SpyTag/SpyCatcher technology.
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Cites
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Cited by
Liu, X., Yang, X., Yang, Z., Luo, J., Tian, X., Liu, K., Kou, S., & Sun, F. (2018) · ACS Applied Nano Materials · doi:10.1021/acsanm.8b00077 · Cited by 32
Metadata
- Authors
- Reddington, S. C., & Howarth, M.
- Venue
- Current Opinion in Chemical Biology
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 29
- Pages
- 94-99
- ISSN
- 1367-5931 (ISSN-L)
- OpenAlex
- W2151007289
- Cited by
- 326
- References
- 35
- FWCI
- 1.59
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
