Versatile Engineered Protein Hydrogels Enabling Decoupled Mechanical and Biochemical Tuning for Cell Adhesion and Neurite Growth
Abstract
Development of engineered protein materials with wide-ranging mechanical strength and stiffness while maintaining the biofunctionality of protein molecules within remains a big challenge. Here we demonstrate the synthesis of protein hydrogels by photochemically cross-linking recombinant mussel foot protein-3 (Mfp3). The hydrogels’ stiffness can be broadly tuned by adjusting the concentration of protein polymers or co-oxidants, or light intensity needed for the chemical cross-linking. The protein polymers were also designed to contain SpyCatcher domains, which enabled postgelation decoration with diverse folded globular proteins under mild physiological conditions. Not only did the resulting hydrogels support the adhesion and proliferation of a variety of cell lines, but they were also able to activate the JAK/STAT3 pathway and induce neurite growth via the covalently immobilized leukemia inhibitory factor (LIF). These results illustrate a new strategy for designing bioactive materials for regenerative neurobiology.
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Recombinant proteins are molecularly well-defined and can be tuned independently for stiffness, viscoelastic behavior, and chemical functionality.
“Recombinant proteins are molecularly well-defined and can be tuned independently for stiffness, viscoelastic behavior, and chemical functionality203–205.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [204]
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Towards organoid culture without Matrigel
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- Authors
- Liu, X., Yang, X., Yang, Z., Luo, J., Tian, X., Liu, K., Kou, S., & Sun, F.
- Affiliations
- Hong Kong University of Science and Technology (HK)
- Corresponding author
- Kou, Songzi
- Sun, Fei
- Venue
- ACS Applied Nano Materials
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-03-26
- Volume
- 1
- Issue
- 4
- Pages
- 1579-1585
- ISSN
- 2574-0970 (ISSN-L)
- OpenAlex
- W2791062651
- Cited by
- 32
- References
- 41
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 5 · 2023: 2 · 2022: 4 · 2021: 6 · 2020: 1 · 2019: 7 · 2018: 3
- FWCI
- 1.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- ACS Applied Nano Materials (publishedVersion)
- Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
- Funders
- Research Grants Council, University Grants Committee (26103915, AoE/M-09/12)
- Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Keywords
- protein hydrogels, photochemical cross-linking, cell adhesion, neurite outgrowth, JAK/STAT3 signaling, leukemia inhibitory factor, bioactive materials
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