Cited by 32Hydrogels: synthesis, properties, applicationsSupramolecular Self-Assembly in MaterialsSilk-based biomaterials and applications

Versatile Engineered Protein Hydrogels Enabling Decoupled Mechanical and Biochemical Tuning for Cell Adhesion and Neurite Growth

Liu, Xiaotian, Yang, Xin, Yang, Zhongguang, Luo, Jiren, Tian, Xiaozhen, Liu, Kai, Kou, Songzi, Sun, Fei·2018·ACS Applied Nano Materials

Abstract

Development of engineered protein materials with wide-ranging mechanical strength and stiffness while maintaining the biofunctionality of protein molecules within remains a big challenge. Here we demonstrate the synthesis of protein hydrogels by photochemically cross-linking recombinant mussel foot protein-3 (Mfp3). The hydrogels’ stiffness can be broadly tuned by adjusting the concentration of protein polymers or co-oxidants, or light intensity needed for the chemical cross-linking. The protein polymers were also designed to contain SpyCatcher domains, which enabled postgelation decoration with diverse folded globular proteins under mild physiological conditions. Not only did the resulting hydrogels support the adhesion and proliferation of a variety of cell lines, but they were also able to activate the JAK/STAT3 pathway and induce neurite growth via the covalently immobilized leukemia inhibitory factor (LIF). These results illustrate a new strategy for designing bioactive materials for regenerative neurobiology.

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Crossref · 10.1021/acsanm.8b00077

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  • Recombinant proteins are molecularly well-defined and can be tuned independently for stiffness, viscoelastic behavior, and chemical functionality.

    “Recombinant proteins are molecularly well-defined and can be tuned independently for stiffness, viscoelastic behavior, and chemical functionality203–205.”

    From Towards organoid culture without Matrigel (2021) · cites this paper as [204]

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Authors
Liu, X., Yang, X., Yang, Z., Luo, J., Tian, X., Liu, K., Kou, S., & Sun, F.
Affiliations
Hong Kong University of Science and Technology (HK)
Corresponding author
  • Kou, Songzi
  • Sun, Fei
Venue
ACS Applied Nano Materials
Published
2018-03-26
Volume
1
Issue
4
Pages
1579-1585
ISSN
2574-0970 (ISSN-L)
OpenAlex
W2791062651
Cited by
32
References
41
Citations by year
2026: 2 · 2025: 2 · 2024: 5 · 2023: 2 · 2022: 4 · 2021: 6 · 2020: 1 · 2019: 7 · 2018: 3
FWCI
1.61
Type
journal article
Language
en
Open access
closed
Locations
  • ACS Applied Nano Materials (publishedVersion)
  • Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
Funders
  • Research Grants Council, University Grants Committee (26103915, AoE/M-09/12)
  • Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
  • Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
Keywords
protein hydrogels, photochemical cross-linking, cell adhesion, neurite outgrowth, JAK/STAT3 signaling, leukemia inhibitory factor, bioactive materials

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