Cited by 5Environmental Toxicology and EcotoxicologyCrustacean biology and ecologyHeavy Metal Pollution Remediation

Short communication: Effects of acute copper exposure on ionic regulation of the freshwater crab Aegla castro

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Rosa, Jheimison Junior da Silva, Martinez, Claudia Bueno dos Reis·2021·Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology

Abstract

Aeglids are unique freshwater decapods whose habitats are being impacted by metallic compounds, such as copper (Cu). Thus, we investigated the effects of acute Cu exposure on ionic regulation of Aegla castro. For this, male specimens in intermolt were collected from a reference stream and acclimated for 5 days in laboratory. After which, crabs were exposed to 11 μg L-1 Cu (Cu11) or only to water (CTR) for 24 h. Hemolymph samples were withdrawn for the determination of Na+, K+, Ca 2+, and Mg 2+ concentrations and the posterior gills removed for the analysis of Na+/K+-ATPase, Ca 2+-ATPase, H+-ATPase, and carbonic anhydrase (CA) activities. Increased Ca 2+ and Mg 2+ hemolymph concentrations were observed in animals from Cu11, when compared with CTR group. In addition, decreased activity of CA was observed in animals exposed to Cu. In the current study, alterations in Ca 2+ and Mg 2+concentrations probably indicate that animals activated exoskeleton reabsorption mechanisms, characteristic of the premolt. Therefore, increased Ca 2+ and Mg 2+ concentrations in hemolymph may indicate that a biochemical signal associated with the molting cycle was triggered by Cu exposure. Despite the known harmful effects of Cu on osmoregulatory enzymes, here we observed decreased activity only in CA. However, decreased activity of CA could trigger both acid-base imbalance and ionic disruption, since CA provides H+ and HCO 3- for intracellular pH maintenance, and underpins Na+ and Cl- for ionic regulation. Therefore, understanding how aeglids respond to metal contamination in laboratory conditions is crucial to assess their potential as an alternative biological model for aquatic ecotoxicology.

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Crossref · 10.1016/j.cbpc.2021.109106

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Authors
Rosa, J. J. D. S., & Martinez, C. B. D. R.
Affiliations
Universidade Estadual de Londrina (BR)
Venue
Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Publisher
Elsevier BV
Published
2021-10
Volume
248
Pages
109106
ISSN
1532-0456 (ISSN-L)
OpenAlex
W3169067334
Cited by
5
References
44
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1
FWCI
0.30
Type
journal article
Language
en
Open access
Locations
  • Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • CAPES
  • CNPq
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Physical Sciences › Environmental Science › Ecology › Crustacean biology and ecology
  • Physical Sciences › Environmental Science › Water Science and Technology › Heavy Metal Pollution Remediation
Keywords
copper toxicity, ion regulation, osmoregulation, carbonic anhydrase, Na+/K+-ATPase, Ca2+-ATPase, H+-ATPase, calcium and magnesium, molting cycle, acid-base balance, freshwater crabs, metal contamination, aquatic ecotoxicology
MeSH terms
Animals, Carbonic Anhydrases, Carbonic Anhydrase Inhibitors, Copper, Brachyura, Gills, Male, Water Pollutants, Chemical, Water-Electrolyte Balance, Biomarkers

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