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Cited by 100Growth Hormone and Insulin-like Growth FactorsClinical Laboratory Practices and Quality ControlPituitary Gland Disorders and Treatments

Laboratory measurement of growth hormone

Popii, Violeta, Baumann, Gerhard·2004·Clinica Chimica Acta
DOI 10.1016/j.cccn.2004.06.007
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Abstract

Growth hormone (GH) measurements are complicated by the heterogeneous nature of GH, as well as by the presence of the GH binding protein in plasma. Several isoforms of GH exist, and specific assays for each are currently either unavailable, impractical, or not clinically indicated. Bioassays include the in vivo assays based on rat weight gain, tibial line widening, or IGF-I generation. In vitro bioassays, based on the proliferation of cell lines expressing the prolactin receptor or GH receptor, are sensitive but prone to nonspecific interference by factors present in serum. Immunoassays (RIA, IRMA, ELISA, and immunofunctional assay design) are widely used in the clinical laboratory because of speed, sensitivity, and convenience. Discrepancies among results rendered by different immunoassays have become more apparent as monoclonal assays have superseded polyclonal assays, presumably because different antibodies recognize different epitopes among the heterogeneous mixture of GH isoforms in serum. Some assays, especially those with short, nonequilibrium incubation times are vulnerable to interference by the GH binding protein present in serum. Recommendations are given for strategies designed to minimize disparity of results obtained by different GH immunoassays applied to serum. Urinary GH measurements, while technically feasible, are of limited clinical utility because of biological variation in urinary GH excretion.

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  • GH can be measured by several different types of assays with results that may differ three-fold (IRMA versus RIA versus ELISA).

    “For example, GH can be measured by several different types of assays with results that may differ three-fold (immunoradiometric assays (IRMA) versus radioimmunoassays (RIA) versus enzyme-linked immunoassays (ELISA)) [51].”

    From Update of Endocrine Dysfunction following Pediatric Traumatic Brain Injury (2015) · cites this paper as [51]

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Authors
Popii, V., & Baumann, G.
Affiliations
Northwestern University (US)
Corresponding author
Baumann, Gerhard
Venue
Clinica Chimica Acta
Publisher
Elsevier BV
Published
2004-12
Volume
350
Issue
1-2
Pages
1-16
ISSN
0009-8981 (ISSN-L)
DOI
10.1016/j.cccn.2004.06.007
OpenAlex
W2016347532
Cited by
100
References
109
Citations by year
2026: 3 · 2025: 1 · 2024: 3 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 2 · 2014: 2 · 2013: 9 · 2012: 13
FWCI
0.85
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Clinica Chimica Acta (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Growth Hormone and Insulin-like Growth Factors
  • Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Pituitary Gland Disorders and Treatments
Keywords
growth hormone, growth hormone binding protein, growth hormone isoforms, bioassay, prolactin receptor, growth hormone receptor, monoclonal antibodies, assay interference, urinary growth hormone, insulin-like growth factor I
MeSH terms
Animals, Antibodies, Monoclonal, Antibody Specificity, Binding Sites, Immunoassay, Growth Hormone, Protein Isoforms

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