Control of embryonic stem cell lineage commitment by core promoter factor, TAF3
Open accessAbstract
Deciphering the molecular basis of pluripotency is fundamental to our understanding of development and embryonic stem cell function. Here, we report that TAF3, a TBP-associated core promoter factor, is highly enriched in ES cells. In this context, TAF3 is required for endoderm lineage differentiation and prevents premature specification of neuroectoderm and mesoderm. In addition to its role in the core promoter recognition complex TFIID, genome-wide binding studies reveal that TAF3 localizes to a subset of chromosomal regions bound by CTCF/cohesin that are selectively associated with genes upregulated by TAF3. Notably, CTCF directly recruits TAF3 to promoter distal sites and TAF3-dependent DNA looping is observed between the promoter distal sites and core promoters occupied by TAF3/CTCF/cohesin. Together, our findings support a new role of TAF3 in mediating long-range chromatin regulatory interactions that safeguard the finely-balanced transcriptional programs underlying pluripotency.
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General TFs such as Taf3 appear to enhance interaction between promoters but had little effect on insulation.
“general TFs such as Taf3 (Liu et al., 2011) appear to enhance interaction between promoters but had little effect on insulation (Figure S5D).”
From Multiscale 3D Genome Rewiring during Mouse Neural Development (2017) · cites this paper as Liu et al., 2011
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References 57 · Cited by 167
- Authors
- Liu, Z., Scannell, D. R., Eisen, M. B., & Tjian, R.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Tjian, Robert
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2011-09
- Volume
- 146
- Issue
- 5
- Pages
- 720-731
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC3191068
- OpenAlex
- W1984015499
- Cited by
- 167
- References
- 57
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 4 · 2023: 5 · 2022: 9 · 2021: 6 · 2020: 11 · 2019: 6 · 2018: 9 · 2017: 9 · 2016: 15 · 2015: 18 · 2014: 22 · 2013: 25 · 2012: 16
- FWCI
- 8.69
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 685 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Cancer Institute (R37CA025417)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- TAF3, embryonic stem cells, pluripotency, lineage commitment, endoderm differentiation, TFIID, core promoter, CTCF, cohesin, DNA looping, long-range chromatin interactions, mesoderm specification, transcriptional regulation
- MeSH terms
- CCCTC-Binding Factor, Cohesins, Animals, Chromosomal Proteins, Non-Histone, Endoderm, Humans, Promoter Regions, Genetic, Repressor Proteins, Teratoma, Transcription, Genetic, Homeodomain Proteins, Gene Expression Regulation, Developmental, Cell Cycle Proteins, Transcription Factor TFIID, TATA-Binding Protein Associated Factors, Cell Proliferation, Mice, Embryonic Stem Cells
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