The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
CENP-T-W-S-X Forms a Unique Centromeric Chromatin Structure with a Histone-like Fold
Open accessAbstract
Summary The multi-protein kinetochore complex must assemble at a specific site on each chromosome to achieve accurate chromosome segregation. Defining the nature of the DNA-protein interactions that specify the position of the kinetochore and provide a scaffold for kinetochore formation remain key goals. Here, we demonstrate that the centromeric histone-fold containing CENP-T-W and CENP-S-X complexes co-assemble to form a stable CENP-T-W-S-X heterotetramer. High-resolution structural analysis of the individual complexes and the heterotetramer reveals similarity to other histone fold-containing complexes including canonical histones within a nucleosome. The CENP-T-W-S-X heterotetramer binds to and supercoils DNA. Mutants designed to compromise heterotetramerization or the DNA-protein contacts around the heterotetramer strongly reduce the DNA binding and supercoiling activities in vitro and compromise kinetochore assembly in vivo. These data suggest that the CENP-T-W-S-X complex forms a unique nucleosome-like structure to generate contacts with DNA, extending the “histone code” beyond canonical nucleosome proteins.
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PHENIX : a comprehensive Python-based system for macromolecular structure solution
Adams, P. D., Afonine, P. V., Bunkóczi, G., Chen, V. B., Davis, I. W., Echols, N., Headd, J. J., Hung, L.-W., Kapral, G. J., Grosse-Kunstleve, R. W., et al. (2010) · Acta Crystallographica Section D Biological Crystallography · doi:10.1107/s0907444909052925 · Cited by 23,716
Cited by
Structure of the human inner kinetochore bound to a centromeric CENP-A nucleosome
Yatskevich, S., Muir, K. W., Bellini, D., Zhang, Z., Yang, J., Tischer, T., Predin, M., Dendooven, T., McLaughlin, S. H., & Barford, D. (2022) · Science · doi:10.1126/science.abn3810 · Cited by 120
Tilocca, B., Balmas, V., Hassan, Z. U., Jaoua, S., & Migheli, Q. (2019) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2019.108265 · Cited by 71
Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere
Dendooven, T., Zhang, Z., Yang, J., McLaughlin, S. H., Schwab, J., Scheres, S. H. W., Yatskevich, S., & Barford, D. (2023) · Science Advances · doi:10.1126/sciadv.adg7480 · Cited by 58
Insects evolved a monomeric histone-fold domain in the CENP-T protein family
Sankaranarayanan, S. R., Ulmer, J., Mørch, A., Ali-Ahmad, A., Sekulić, N., & Drinnenberg, I. A. (2025) · EMBO Reports · doi:10.1038/s44319-025-00603-5 · Cited by 2
Metadata
- Authors
- Nishino, T., Takeuchi, K., Gascoigne, K. E., Suzuki, A., Hori, T., Oyama, T., Morikawa, K., Cheeseman, I. M., & Fukagawa, T.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2012-02
- Volume
- 148
- Issue
- 3
- Pages
- 487-501
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2010280142
- Cited by
- 263
- References
- 1
- FWCI
- 12.36
- Type
- journal article
- Language
- en
- Open access
- bronze
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