The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Global Analysis of mRNA Isoform Half-Lives Reveals Stabilizing and Destabilizing Elements in Yeast
Open accessAbstract
SUMMARY We measured half-lives of 21,248 mRNA 3′ isoforms in yeast by rapidly depleting RNA polymerase II from the nucleus and performing direct RNA sequencing throughout the decay process. Interestingly, half-lives of mRNA isoforms from the same gene, including nearly identical isoforms, often vary widely. Based on clusters of isoforms with different half-lives, we identify hundreds of sequences conferring stabilization or destabilization upon mRNAs terminating downstream. One class of stabilizing element is a polyU sequence that can interact with poly(A) tails, inhibit the association of poly(A)-binding protein, and confer increased stability upon introduction into ectopic transcripts. More generally, destabilizing and stabilizing elements are linked to the propensity of the poly(A) tail to engage in double-stranded structures. Isoforms engineered to fold into 3′ stem-loop structures not involving the poly(A) tail exhibit even longer half-lives. We suggest that double-stranded structures at 3′ ends are a major determinant of mRNA stability.
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Alternative polyadenylation of mRNA precursors
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Codon Optimality Is a Major Determinant of mRNA Stability
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“However, these features regulate mRNA stability predominantly in a transcript-specific manner through binding of regulatory factors and cannot account for the wide variation in half-lives observed across the entire transcriptome (Geisberg et al., 2014).”
BackgroundInfluentialSingle-cell RNA-seq: advances and future challenges
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Engineered pegRNAs improve prime editing efficiency
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Metadata
- Authors
- Geisberg, J. V., Moqtaderi, Z., Fan, X., Ozsolak, F., & Struhl, K.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2014-02
- Volume
- 156
- Issue
- 4
- Pages
- 812-824
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2033152634
- Cited by
- 270
- References
- 46
- FWCI
- 10.34
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Geisberg, J. V., Moqtaderi, Z., Fan, X., Ozsolak, F., & Struhl, K. (2014). Global Analysis of mRNA Isoform Half-Lives Reveals Stabilizing and Destabilizing Elements in Yeast. Cell. https://doi.org/10.1016/j.cell.2013.12.026
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