The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
DNA Demethylation Dynamics in the Human Prenatal Germline
Open accessAbstract
Summary Global DNA demethylation in humans is a fundamental process that occurs in pre-implantation embryos and reversion to naïve ground state pluripotent stem cells (PSCs). However the extent of DNA methylation reprogramming in human germline cells is unknown. Here we performed whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-Seq) of human prenatal germline cells from 53–137 days of development. We discovered that the transcriptome and methylome of human germline is distinct from both human PSCs and the inner cell mass (ICM) of human blastocysts. Using this resource to monitor the outcome of global DNA demethylation with reversion of primed PSCs to the naïve ground state, we uncovered hotspots of ultralow methylation at transposons that are protected from demethylation in the germline and ICM. Taken together the human germline serves as a valuable in vivo tool for monitoring the epigenome of cells that have emerged from a global DNA demethylation event.
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WGCNA: an R package for weighted correlation network analysis
Langfelder, P., & Horvath, S. (2008) · BMC Bioinformatics · doi:10.1186/1471-2105-9-559 · Cited by 27,252
GREAT improves functional interpretation of cis-regulatory regions
McLean, C. Y., Bristor, D., Hiller, M., Clarke, S. L., Schaar, B. T., Lowe, C. B., Wenger, A. M., & Bejerano, G. (2010) · Nature Biotechnology · doi:10.1038/nbt.1630 · Cited by 4,502
Stage-Specific Roles for Tet1 and Tet2 in DNA Demethylation in Primordial Germ Cells
Vincent, J. J., Huang, Y., Chen, P.-Y., Feng, S., Calvopiña, J. H., Nee, K., Lee, S. A., Le, T., Yoon, A. J., Faull, K., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.01.016 · Cited by 169
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Cited by
TET-mediated active DNA demethylation: mechanism, function and beyond
Wu, X., & Zhang, Y. (2017) · Nature Reviews Genetics · doi:10.1038/nrg.2017.33 · Cited by 1,661
Dynamic stem cell states: Naive to primed pluripotency in rodents and humans
Weinberger, L., Ayyash, M., Novershtern, N., & Hanna, J. H. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2015.28 · Cited by 622
Single-Cell RNA-Seq Analysis Maps Development of Human Germline Cells and Gonadal Niche Interactions
Li, L., Dong, J., Yan, L., Yong, J., Liu, X., Hu, Y., Fan, X., Wu, X., Guo, H., Wang, X., et al. (2017) · Cell Stem Cell · doi:10.1016/j.stem.2017.03.007 · Cited by 561
Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis
Chen, Y., Zheng, Y., Gao, Y., Lin, Z., Yang, S., Wang, T., Wang, Q., Xie, N., Hua, R., Liu, M., et al. (2018) · Cell Research · doi:10.1038/s41422-018-0074-y · Cited by 420
Profiling genome-wide DNA methylation
Yong, W.-S., Hsu, F.-M., & Chen, P.-Y. (2016) · Epigenetics & Chromatin · doi:10.1186/s13072-016-0075-3 · Cited by 374
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Metadata
- Authors
- Gkountela, S., Zhang, K. X., Shafiq, T. A., Liao, W.-W., Hargan-Calvopiña, J., Chen, P.-Y., & Clark, A. T.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2015-06
- Volume
- 161
- Issue
- 6
- Pages
- 1425-1436
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2098711385
- Cited by
- 349
- References
- 3
- FWCI
- 16.08
- Type
- journal article
- Language
- en
- Open access
- bronze
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