A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface
Open accessAbstract
The centrosome is the primary microtubule organizing center of the cells and templates the formation of cilia, thereby operating at a nexus of critical cellular functions. Here, we use proximity-dependent biotinylation (BioID) to map the centrosome-cilium interface; with 58 bait proteins we generate a protein topology network comprising >7,000 interactions. Analysis of interaction profiles coupled with high resolution phenotypic profiling implicates a number of protein modules in centriole duplication, ciliogenesis, and centriolar satellite biogenesis and highlights extensive interplay between these processes. By monitoring dynamic changes in the centrosome-cilium protein interaction landscape during ciliogenesis, we also identify satellite proteins that support cilia formation. Systematic profiling of proximity interactions combined with functional analysis thus provides a rich resource for better understanding human centrosome and cilia biology. Similar strategies may be applied to other complex biological structures or pathways.
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BioID has been used extensively in vitro to define interacting protein partners and proteins in subcellular compartments, but its application in vivo is limited.
“BioID has been used extensively in vitro [162–166] to define interacting protein partners and proteins in subcellular compartments, but its application in vivo is limited.”
From Systems-based proteomics to resolve the biology of Alzheimer’s disease beyond amyloid and tau (2021) · cites this paper as [163]
We find 126 genes with 143 HHMCs that putatively interact with proteins at the centrosome-cilium interface.
“We find 126 genes (Table S9) with 143 HHMCs that putatively interact with proteins at the centrosome-cilium interface71.”
From A catalog of single nucleotide changes distinguishing modern humans from archaic hominins (2019) · cites this paper as [71]
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- Authors
- Gupta, G. D., Coyaud, É., Gonçalves, J., Mojarad, B. A., Liu, Y., Wu, Q., Gheiratmand, L., Comartin, D., Tkach, J. M., Cheung, S. W. T., Bashkurov, M., Hasegan, M., Knight, J. D., Lin, Z.-Y., Schueler, M., Hildebrandt, F., Moffat, J., Gingras, A.-C., Raught, B., & Pelletier, L.
- Affiliations
- Mount Sinai Hospital (CA)
- University Health Network (CA)
- Boston Children's Hospital (US)
- University of Toronto (CA)
- Corresponding author
- Raught, Brian
- Pelletier, Laurence
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 163
- Issue
- 6
- Pages
- 1484-1499
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC5089374
- OpenAlex
- W2188873428
- Cited by
- 538
- References
- 47
- Citations by year
- 2026: 24 · 2025: 58 · 2024: 35 · 2023: 42 · 2022: 56 · 2021: 53 · 2020: 70 · 2019: 60 · 2018: 62 · 2017: 47 · 2016: 29 · 2014: 1
- FWCI
- 31.43
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 368 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Krembil Foundation
- Instituto Nacional de Ciência e Tecnologia para Excitotoxicidade e Neuroproteção (SFRH/BPD/75847/2011)
- Natural Sciences and Engineering Research Council of Canada (MOP-123468, MOP-130507, MOP119289, MOP130340, ORF-RE5, RGPIN-2014-06434, RGPIN-355644-2008)
- Canadian Institutes of Health Research (OGB 137413)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Biotin and Related Studies
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Keywords
- centrosome, cilia, protein-protein interaction network, centriole duplication, ciliogenesis, centriolar satellites, microtubule organizing center
- MeSH terms
- Cell Cycle, Cilia, Humans, Centrosome, Biotinylation, Microtubule-Organizing Center, Protein Interaction Maps
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