The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Magnesium Flux Modulates Ribosomes to Increase Bacterial Survival
Open accessAbstract
Bacteria exhibit cell-to-cell variability in their resilience to stress, for example, following antibiotic exposure. Higher resilience is typically ascribed to "dormant" non-growing cellular states. Here, by measuring membrane potential dynamics of Bacillus subtilis cells, we show that actively growing bacteria can cope with ribosome-targeting antibiotics through an alternative mechanism based on ion flux modulation. Specifically, we observed two types of cellular behavior: growth-defective cells exhibited a mathematically predicted transient increase in membrane potential (hyperpolarization), followed by cell death, whereas growing cells lacked hyperpolarization events and showed elevated survival. Using structural perturbations of the ribosome and proteomic analysis, we uncovered that stress resilience arises from magnesium influx, which prevents hyperpolarization. Thus, ion flux modulation provides a distinct mechanism to cope with ribosomal stress. These results suggest new approaches to increase the effectiveness of ribosome-targeting antibiotics and reveal an intriguing connection between ribosomes and the membrane potential, two fundamental properties of cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“These data, together with recent publications highlighting the connection between Mg2+ homeostasis and translation (29, 33, 34), support the idea that a decrease in Mg2+ during osmoadaptation could impair translation and thereby delay the resumption of growth.”
From A Central Role for Magnesium Homeostasis during Adaptation to Osmotic Stress (2022) · cites this paper as [34]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Fiji: an open-source platform for biological-image analysis
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., et al. (2012) · Nature Methods · doi:10.1038/nmeth.2019 · Cited by 66,890
Hodgkin, A. L., & Huxley, A. F. (1952) · The Journal of Physiology · doi:10.1113/jphysiol.1952.sp004764 · Cited by 23,191
The Complete Atomic Structure of the Large Ribosomal Subunit at 2.4 Å Resolution
Ban, N., Nissen, P., Hansen, J., Moore, P. B., & Steitz, T. A. (2000) · Science · doi:10.1126/science.289.5481.905 · Cited by 3,327
Bacterial Persistence as a Phenotypic Switch
Balaban, N. Q., Merrin, J., Chait, R., Kowalik, L., & Leibler, S. (2004) · Science · doi:10.1126/science.1099390 · Cited by 2,952
Trainable Weka Segmentation: a machine learning tool for microscopy pixel classification
Arganda‐Carreras, I., Kaynig, V., Rueden, C., Eliceiri, K. W., Schindelin, J., Cardona, A., & Seung, H. S. (2017) · Bioinformatics · doi:10.1093/bioinformatics/btx180 · Cited by 2,452
Carter, A. P., Clemons, W. M., Brodersen, D. E., Morgan-Warren, R. J., Wimberly, B. T., & Ramakrishnan, V. (2000) · Nature · doi:10.1038/35030019 · Cited by 1,615
Functional roles for noise in genetic circuits
Eldar, A., & Elowitz, M. B. (2010) · Nature · doi:10.1038/nature09326 · Cited by 1,482
Schaechter, M., MaalOe, O., & Kjeldgaard, N. O. (1958) · Journal of General Microbiology · doi:10.1099/00221287-19-3-592 · Cited by 1,435
Phenotypic Diversity, Population Growth, and Information in Fluctuating Environments
Kussell, E. L., & Leibler, S. (2005) · Science · doi:10.1126/science.1114383 · Cited by 1,357
Cellular Decision Making and Biological Noise: From Microbes to Mammals
Balázsi, G., van Oudenaarden, A., & Collins, J. J. (2011) · Cell · doi:10.1016/j.cell.2011.01.030 · Cited by 1,017
Cited by
Sorted by citations
The Microbiologist’s Guide to Membrane Potential Dynamics
Benarroch, J. M., & Asally, M. (2020) · Trends in Microbiology · doi:10.1016/j.tim.2019.12.008 · Cited by 333
Stratford, J. P., Edwards, C. L. A., Ghanshyam, M. J., Malyshev, D., Delise, M. A., Hayashi, Y., & Asally, M. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1901788116 · Cited by 243
Molecular Mechanisms for Bacterial Potassium Homeostasis
Stautz, J., Hellmich, Y., Fuss, M. F., Silberberg, J. M., Devlin, J. R., Stockbridge, R. B., & Hänelt, I. (2021) · Journal of Molecular Biology · doi:10.1016/j.jmb.2021.166968 · Cited by 189
Magnesium Is a Key Player in Neuronal Maturation and Neuropathology
Yamanaka, R., Shindo, Y., & Oka, K. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20143439 · Cited by 184
Bruni, G. N., & Kralj, J. M. (2020) · eLife · doi:10.7554/elife.58706 · Cited by 126
Metadata
- Authors
- Lee, D.-Y. D., Galera-Laporta, L., Bialecka-Fornal, M., Moon, E. C., Shen, Z., Briggs, S. P., Garcia-Ojalvo, J., & Süel, G. M.
- Affiliations
- University of California San Diego (US)
- Universitat Pompeu Fabra (ES)
- Corresponding author
- Süel, Gürol M.
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2019-04
- Volume
- 177
- Issue
- 2
- Pages
- 352-360.e13
- ISSN
- 0092-8674 (ISSN-L)
- PMC
- PMC6814349
- OpenAlex
- W2922356758
- Cited by
- 147
- References
- 46
- Citations by year
- 2026: 27 · 2025: 24 · 2024: 20 · 2023: 15 · 2022: 24 · 2021: 23 · 2020: 7 · 2019: 7
- FWCI
- 9.18
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Repositori digital de la UPF (Universitat Pompeu Fabra) (acceptedVersion)
- RECERCAT (Consorci de Serveis Universitaris de Catalunya) (submittedVersion)
- Funders
- Spanish Ministry of Economy and Competitiveness
- FEDER (FIS2015-66503-C3-1-P)
- ICREA
- Maria de Maeztu Program for Units of Excellence in Research and Development (MDM-2014-0370)
- San Diego Center for Systems Biology
- NIH (P50 GM085764)
- National Institute of General Medical Sciences (R01 GM121888)
- Howard Hughes Medical Institute
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- magnesium influx, ribosome-targeting antibiotics, membrane hyperpolarization, Bacillus subtilis, cell-to-cell variability, antibiotic tolerance
- MeSH terms
- Bacterial Outer Membrane, Bacillus subtilis, Bacterial Proteins, Magnesium, Ribosomal Proteins, Ribosomes, Proteomics
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
