The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures
Open accessAbstract
SUMMARY A single mouse blastomere from until 8-cell embryo can generate an entire blastocyst. Whether laboratory cultured cells retain a similar generative capacity remains unknown. Starting from a single stem cell type, extended pluripotent stem (EPS) cells, we established a 3D differentiation system that enabled the generation of blastocyst-like structures (EPS-blastoids) through lineage segregation and self-organization. EPS-blastoids resembled blastocysts in morphology and cell lineage allocation and recapitulated key morphogenetic events during preimplantation and early postimplantation development in vitro. Upon transfer, some EPS-blastoids underwent implantation, induced decidualization, and generated live, albeit disorganized, tissues in utero. Single-cell and bulk RNA-sequencing analysis revealed that EPS-blastoids contained all three blastocyst cell lineages and shared transcriptional similarity with natural blastocysts. We also provide proof-of-concept that EPS-blastoids can be generated from adult cells via cellular reprogramming. EPS-blastoids provide a unique platform for studying early embryogenesis and pave the way to create viable synthetic embryos using cultured cells.
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A scalable platform for EPSC-induced MSC extracellular vesicles with therapeutic potential
Gong, S., Li, N., Peng, Q., Wang, F., Du, R., Zhang, B., Wang, J., Han, L., Zhang, Y., Ning, Z., et al. (2025) · Stem Cell Research & Therapy · doi:10.1186/s13287-025-04507-y · Cited by 28
Metadata
- Authors
- Li, R., Zhong, C., Yu, Y., Liu, H., Sakurai, M., Yu, L., Min, Z., Shi, L., Wei, Y., Takahashi, Y., Liao, H.-K., Qiao, J., Deng, H., Nuñez-Delicado, E., Rodriguez Esteban, C., Wu, J., & Izpisua Belmonte, J. C.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2019-10
- Volume
- 179
- Issue
- 3
- Pages
- 687-702.e18
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2980793885
- Cited by
- 267
- References
- 64
- FWCI
- 8.74
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Li, R., Zhong, C., Yu, Y., Liu, H., Sakurai, M., Yu, L., Min, Z., Shi, L., Wei, Y., Takahashi, Y., Liao, H.-K., Qiao, J., Deng, H., Nuñez-Delicado, E., Rodriguez Esteban, C., Wu, J., & Izpisua Belmonte, J. C. (2019). Generation of Blastocyst-like Structures from Mouse Embryonic and Adult Cell Cultures. Cell. https://doi.org/10.1016/j.cell.2019.09.029
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