Cellular and Molecular Probing of Intact Human Organs
Zhao, Shan, Todorov, Mihail Ivilinov, Cai, Ruiyao, -Maskari, Rami AI, Steinke, Hanno, Kemter, Elisabeth, Mai, Hongcheng, Rong, Zhouyi +15 more·2020·Cell
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- Light sheet fluorescence microscopy of cleared human eyesMarie Darche, Ysé Borella, Anna Verschueren, Ivana Gantar, Stéphane Pagès, Laura Batti, Michel Pâques · 2023 · Communications Biology
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- The Potential of OMICs Technologies for the Treatment of Immune-Mediated Inflammatory DiseasesCharles Gwellem Anchang, Cong Xu, Maria Gabriella Raimondo, Raja Narayana Atreya, Andreas Maier, Georg Andreas Schett, Vasily Zaburdaev, Simon Rauber, Andreas Ramming · 2021 · International Journal of Molecular Sciences
- Optical angiography for diabetes-induced pathological changes in microvascular structure and function: An overviewJingtan Zhu, Dongyu Li, Tingting Yu, Dan Yan Zhu · 2021 · Journal of Innovative Optical Health Sciences
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- MarShie: a clearing protocol for 3D analysis of single cells throughout the bone marrow at subcellular resolutionTill Fabian Mertens, Alina Liebheit, Johanna Ehl, Ralf Köhler, Asylkhan A. Rakhymzhan, Andrew Woehler, Lukas Katthän, G. Ebel, Wjatscheslaw Liublin, Ana Kasapi et al. · 2024 · Nature Communications
- Multi-omics and 3D-imaging reveal bone heterogeneity and unique calvaria cells in neuroinflammationZeynep Ilgin Kolabas, Louis Benedikt Kuemmerle, Robert Perneczky, Benjamín Förstera, Maren Büttner, Özüm Şehnaz Çalışkan, Mayar Ali, Zhouyi Rong, Hongcheng Mai, Selina Hummel et al. · 2021 · bioRxiv (Cold Spring Harbor Laboratory)
- Diffusion tensor imaging‐based studies at the group‐level applied to animal models of neurodegenerative diseasesMüller, Hans-Peter, Roselli, Francesco, Rasche, Volker, Kassubek, Jan · 2020 · Frontiers in Neuroscience
- High-Throughput Strategy for Profiling Sequential Section With Multiplex Staining of Mouse BrainSiqi Chen, Zhixiang Liu, Anan Li, Hui Gong, Ben Long, Xiangning Li · 2021 · Frontiers in Neuroanatomy
- Unlocking the potential of large-scale 3D imaging with tissue clearing techniquesEtsuo A. Susaki · 2024 · Microscopy
- Perspectives in systems nephrologyMaja T. Lindenmeyer, Fadhl Alakwaa, Michael P. Rose, Matthias Kretzler · 2021 · Cell and Tissue Research
- Dec-DISCO: decolorization DISCO clearing for seeing through the biological architectures of heme-rich organsJingtan Zhu, Yilin Ma, Jianyi Xu, Yusha Li, Peng Wan, Yisong Qi, Tingting Yu, Dan Yan Zhu · 2021 · Biomedical Optics Express
- Three-dimensional Imaging Coupled with Topological Quantification Uncovers Retinal Vascular Plexuses Undergoing ObliterationChih‐Chiang Chang, Alison Chu, Scott Meyer, Michel M. Sun, Parinaz Abiri, Kyung In Baek, Varun Gudapati, Xili Ding, Pierre Guihard, Yichen Ding et al. · 2020 · Theranostics
- The New Era of Three-Dimensional Histoarchitecture of the Human EndometriumManako Yamaguchi, Kosuke Yoshihara, Nozomi Yachida, Kazuaki Suda, Ryo Tamura, Tatsuya Ishiguro, Takayuki Enomoto · 2021 · Journal of Personalized Medicine
- Multiplexed and scalable cellular phenotyping toward the standardized three-dimensional human neuroanatomyTatsuya C. Murakami, Nathaniel Heintz · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
- 3D light-sheet fluorescence microscopy in preclinical and clinical drug discoveryUrmas Roostalu, Henrik H. Hansen, Jacob Hecksher‐Sørensen · 2024 · Drug Discovery Today
- Advances of novel hydrogels in the healing process of alveolar socketsWenqing Yu, Liwei Hu, Yige Wei, Chengyu Xue, Yunfei Liu, Huixu Xie · 2025 · Biomaterials Advances
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- Tissue clearing and 3D imaging – putting immune cells into contextJulian I. Hofmann, Selina Jessica Keppler · 2021 · Journal of Cell Science
- SUMIC: A Simple Ultrafast Multicolor Immunolabelling and Clearing Approach for Whole-Organ and Large Tissue 3D ImagingLincoln Biswas, Junyu Chen, Jessica E. De Angelis, Alexandros Chatzis, Jagdeep Nanchahal, Michael Loran Dustin, Saravana Kumar Ramasamy, Anjali P. Kusumbe · 2021 · bioRxiv (Cold Spring Harbor Laboratory)
- Tissue Optical Clearing: State of the Art and ProspectsElina Alekseevna Genina · 2022 · Diagnostics
- Increased Multiplexity in Optical Tissue Clearing-Based Three-Dimensional Immunofluorescence Microscopy of the Tumor Microenvironment by Light-Emitting Diode PhotobleachingJingtian Zheng, Yi-Chien Wu, Evan H. Phillips, Xiaoying Cai, Xu Wang, Seung Young Lee · 2024 · Laboratory Investigation
- Whole‐Tumor Clearing and Imaging of Intratumor Microbiota in Three Dimensions with miCDaL StrategyYuezhou Wang, Zile Jiang, Kai Zhang, Huimin Tang, Guimei Wang, Jinshan Gao, Guanghui He, Baoyue Liang, Li Li, Chaoyong James Yang et al. · 2024 · Advanced Science
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- METGAN: Generative Tumour Inpainting and Modality Synthesis in Light Sheet MicroscopyIzabela Horvath, Johannes Christian Paetzold, Oliver Schoppe, Rami Al-Maskari, Ivan Ezhov, Suprosanna Shit, Hongwei Li, Ali Ertürk, Bjoern H. Menze · 2022 · IEEE Winter Conference on Applications of Computer Vision
- Recent progress in optical clearing of eye tissuesWenhan Lu, Zhenle Pei, Wei Hu, Chen Tan, Xiaoyu Tong, Yi Feng, Xinghuai Sun · 2021 · Experimental Eye Research
- Hierarchical Random Walker Segmentation for Large Volumetric Biomedical ImagesDominik Drees, Florian Eilers, Xiaoyi Jiang · 2022 · IEEE Transactions on Image Processing
- Maximizing content across scales: Moving multimodal microscopy and mesoscopy toward molecular imagingSebastian Munck, Jim Swoger, Montserrat Coll‐Lladó, Nicola Gritti, Greetje Vande Velde · 2021 · Current Opinion in Chemical Biology
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- Volume imaging to interrogate cancer cell-tumor microenvironment interactions in space and timeJorge Almagro, Hendrik A. Messal · 2023 · Frontiers in Immunology
- Mapping cellular targets of covalent cancer drugs in the entire mammalian bodyZhengyuan Pang, Verina H. Leung, Cailynn C. Wang, Ahmadreza Attarpour, Anthony Rinaldi, Hanbing Shen, María Dolores Moya-Garzón, Logan H. Sigua, Claire Rammel, Alexandra Selke et al. · 2025 · Cell
- COMBINe enables automated detection and classification of neurons and astrocytes in tissue-cleared mouse brainsYuheng Cai, Xuying Zhang, Chen Li, H. Troy Ghashghaei, Alon Greenbaum · 2023 · Cell Reports Methods
- Three-dimensional mapping in multi-samples with large-scale imaging and multiplexed post stainingSiqi Chen, Guangcai Liu, Anan Li, Zhixiang Liu, Ben Long, Xiao‐Quan Yang, Hui Gong, Xiangning Li · 2023 · Communications Biology
- Mapping the arterial vascular network in an intact human kidney using hierarchical phase-contrast tomographyShahrokh Rahmani, Daniyal J. Jafree, Peter David Lee, Paul Tafforeau, Joseph Brunet, Sonal Nandanwar, Yang Zhou, Joseph Jacob, Alexandre Bellier, Maximilian Ackermann et al. · 2025 · npj Imaging
- Tissue Clearing Approaches in AtherosclerosisTing Sun, Yuanfang Li, Benjamín Förstera, Karen Stanic, Shu Lu, Sabine Steffens, Changjun Yin, Ali Ertürk, Remco T. A. Megens, Christian Weber et al. · 2022 · Methods in molecular biology
- Applications of tissue clearing in the spinal cordTing Tian, Xiaoguang Li · 2020 · European Journal of Neuroscience
- Brown adipose tissue secretes OLFM4 to coordinate sensory and sympathetic innervation via Schwann cellsMingqiang Lai, Wu Zhou, Wenchong Zou, Lianlian Qiu, Zhaoyu Liang, Wanyi Chen, Yiqing Wang, Bin Guo, Chaoran Zhao, Zhisheng Zhang et al. · 2025 · Nature Communications
- The Human Organ AtlasClaire Walsh, Joseph Brunet, David Stansby, G. Gaisné, Yang Zhou, Maximilian Ackermann, Alexandre Bellier, Camille Berruyer, Axel Bocciarelli, Marjolaine Bodin et al. · 2026 · Science Advances
- Testing the suitability of neuroanatomical tracing method in human fetuses with long years of postmortem delayNail Can Öztürk, Turan Koç · 2022 · Surgical and Radiologic Anatomy
- Self-assembly of three-dimensional liver organoids: virtual reconstruction via endocytosed polymer dots for refactoring the fine structureZe Zhang, Yuyang Wu, Zhilu Xuan, Haotian Xu, Shengyan Yin, Zihui Meng · 2023 · Biomaterials Science
- Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brainYa‐Hui Lin, Li-Wen Wang, Yen‐Hui Chen, Yi-Chieh Chan, Shang‐Hsiu Hu, Sheng‐Yan Wu, Chi‐Shiun Chiang, Guan‐Jie Huang, Shang‐Da Yang, Shi‐Wei Chu et al. · 2024 · eLife
- Tissue clearing and its application in dental researchLingxi Meng, Xinyu Song, Junyi Wang, Wenxuan Shi, Liheng Gao, Xinquan Jiang, Wenjie Zhang · 2024 · BMEMat
- Quantitative cytoarchitectural phenotyping of deparaffinized human brain tissuesDanila Di Meo, Michele Sorelli, Josephine Ramazzotti, Franco Cheli, Samuel Bradley, Laura Perego, Beatrice Lorenzon, Giacomo Mazzamuto, Aron Emmi, Andrea Porzionato et al. · 2025 · Communications Biology
- Chemical fluorescence-based dye staining for 3-dimensional histopathology analysisHyun Jung Kim, Jeongah Kim, Jungyoon Choi, Woong Sun · 2022 · Animal Cells and Systems
- A Cellular Ground Truth to Develop MRI Signatures in Glioma Models by Correlative Light Sheet Microscopy and Atlas-Based CoregistrationKatharina Schregel, Lennart Heinz, Jessica Hunger, Chenchen Pan, Julia Bode, Manuel Fischer, Volker Jörg Friedrich Sturm, Varun Venkataramani, Kianush Karimian Jazi, Dennis Alexander Agardy et al. · 2023 · Journal of Neuroscience
- Approaching Peak Ground TruthFlorian Kofler, Johannes Wahle, Ivan Ezhov, Sophia J. Wagner, Rami Al-Maskari, Emilia Agnieszka Gryska, Mihail Ivilinov Todorov, Christina Bukas, Felix Meissen, Tingying Peng et al. · 2023 · IEEE International Symposium on Biomedical Imaging (ISBI)
- The Human Lung Cell Atlas: a transformational resource for cells of the respiratory systemKerstin B. Meyer, Anna Wilbrey-Clark, Martijn C. Nawijn, Sarah Amalia Teichmann · 2021 · European Respiratory Society eBooks
- blob loss: instance imbalance aware loss functions for semantic segmentationFlorian Kofler, Suprosanna Shit, Ivan Ezhov, Lucas Fidon, Izabela Horvath, Rami Al-Maskari, Hongwei Li, Harsharan S. Bhatia, Timo Loehr, Marie Piraud et al. · 2022 · Zurich Open Repository and Archive (University of Zurich)
- Decoding the Mouse Spinal Cord Locomotor Neural Network Using Tissue Clearing, Tissue Expansion and Tiling Light Sheet Microscopy TechniquesRuili Feng, Jiongfang Xie, Jing Lü, Huijie Hu, Yanlu Chen, Dongyue Wang, Liang Gao · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
- Mapping the blood vasculature in an intact human kidney using hierarchical phase-contrast tomographyShahrokh Rahmani, Daniyal J. Jafree, Peter David Lee, Paul Tafforeau, Joseph Brunet, Sonal Nandanwar, Joseph Jacob, Alexandre Bellier, Maximilian Ackermann, Danny David Jonigk et al. · 2023 · bioRxiv (Cold Spring Harbor Laboratory)
- Tissue Clearing and Its Application in Nanoparticle DevelopmentZhouyi Rong, Ali Ertürk, Yamei Tang, Hongcheng Mai · 2025 · Small
- An open-source photobleacher for fluorescence imaging of large pigment-rich tissuesTatsuya C. Murakami, Nick Belenko, Griffin Dennis, Cuidong Wang, Maria Esterlita Siantoputri, Yurie Maeda, Christina Pressl, Nathaniel Heintz · 2025 · Proceedings of the National Academy of Sciences
- A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imagingKitae Kim, Kwangbae Lee, Taehoon Kang, Jungmihn Lee, Wonseok Lee, Ji Yeoun Lee, Sunghoe Chang · 2025 · Experimental & Molecular Medicine
- Artificial intelligence-driven whole-brain cell mapping with highly multiplexed in situ hybridizationTatsuya C. Murakami, Meng Xia, Yurie Maeda, Yuejia Yin, Paolo Emilio Barbano, Ziyi Lin, Tomoyuki Mano, Kazuki Tainaka, Sam Reiter, Nathaniel Heintz · 2026 · Neuron
- aDISCO: A clearing method to enable 3D microscopy of large archival paraffin-embedded human tissue blocksAnna Maria Reuss, Dominik Groos, Martina Cerisoli, Lena Nordberg, Lukas D. Frick, Fabian F. Voigt, Nikita Vladimirov, Philipp Bethge, Regina Reimann, Fritjof Helmchen et al. · 2026 · Science Advances
- Brain-wide immunolabeling and tissue clearing applications for engram researchAlessandra Franceschini, Michelle Jin, Claire W. Chen, Ludovico Silvestri, Alessia Mastrodonato, C. Denny · 2025 · Neurobiology of Learning and Memory
- ADAPT-3D:accelerated deep adaptable processing of tissue for 3-dimensional fluorescence tissue imaging for research and clinical settingsDaniel D. Lee, Kevin A. Telfer, Deanna L. Davis, Leon C. D. Smyth, Rahul Prashanth Ravindran, Rafael Sanguinetti Czepielewski, Christopher G. Huckstep, Siling Du, Kento Kurashima, Ajay Kumar Jain et al. · 2025 · Scientific Reports
- Seeing the Full Picture: Advances in 3D Cell Culture MicroscopyJoseph Martin · 2020 · BioTechniques
- Proteomics of spatially identified tissues in whole organsHarsharan S. Bhatia, Andreas‐David Brunner, Zhouyi Rong, Hongcheng Mai, Marvin Thielert, Rami Al-Maskari, Johannes Christian Paetzold, Florian Kofler, Mihail Ivilinov Todorov, Mayar Ali et al. · 2021 · bioRxiv (Cold Spring Harbor Laboratory)
- Preparation of large biological samples for high-resolution, hierarchical, multi-modal imagingJoseph Brunet, Claire Walsh, Willi Linus Wagner, Alexandre Bellier, Christopher Werlein, Sebastian Marussi, Danny David Jonigk, Stijn E. Verleden, Maximilian Ackermann, Peter David Lee et al. · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
- Three-dimensional imaging and single-cell transcriptomics of the human kidney implicate perturbation of lymphatics in alloimmunityDaniyal J. Jafree, Benjamin J. Stewart, Maria Kolatsi‐Joannou, Benjamin Michael Davis, Hannah J Mitchell, Lauren G. Russell, Lucía Marinas del Rey, William J. Mason, Byung Il Lee, Lauren Heptinstall et al. · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
- Efficient image analysis for large-scale next generation histopathology using pAPRicaJules Scholler, Joel Jonsson, Tomás Jordá-Siquier, Ivana Gantar, Laura Batti, Bevan L. Cheeseman, Stéphane Pagès, Ivo F. Sbalzarini, Christophe M. Lamy · 2023 · bioRxiv (Cold Spring Harbor Laboratory)
- Whole mouse body histology using standard IgG antibodiesHongcheng Mai, Jie Luo, Luciano Hoeher, Rami Al-Maskari, Izabela Horvath, Johannes Christian Paetzold, Mihail Ivilinov Todorov, Farida Hellal, Ali Ertürk · 2023 · bioRxiv (Cold Spring Harbor Laboratory)
- Multidimensional Analysis of the Adult Human Heart in Health and Disease using Hierarchical Phase-Contrast Tomography (HiP-CT)Joseph Brunet, Andrew C. Cook, Claire Walsh, James J. Cranley, Paul Tafforeau, Klaus Engel, Camille Berruyer, E. Burke O’Leary, Alexandre Bellier, Ryo Torii et al. · 2023 · bioRxiv (Cold Spring Harbor Laboratory)
- A deep-learning framework reveals whole-body perturbations at cell levelKaltenecker, Doris, Horvath, Izabela, Al-Maskari, Rami, Chen, Ying, Kolabas, Zeynep Ilgin, Hoeher, Luciano, Todorov, Mihail, Minde, David-Paul, Kapoor, Saketh, Turhan, Sena Gül et al. · 2026 · Nature
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- Three-dimensional quantitative tissue clearing reveals differences in osteovascular niche of aged and young human mesenchymal stromal cellsNelson Tsz Long Chu, Ostap A. Dregval, Yu-Wei Chang, Emil Kriukov, Xin Tian, Xin Liu, Dana Trompet, Misty Shuo Zhang, Lei Li, Zhong Li et al. · 2026 · Nature Biomedical Engineering
- Methods for dynamic and whole volume imaging of the zebrafish heartIsaac Bakis, Yuhan Sun, Laila M. Abd Elmagid, Xidi Feng, Mher Garibyan, Joycelyn K. Yip, Fang Zhou Yu, Sayali Chowdhary, Gerardo Esteban Fernandez, Jingli Cao et al. · 2023 · Developmental Biology
- Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brainYa‐Hui Lin, Li-Wen Wang, Yen‐Hui Chen, Yi-Chieh Chan, Shang‐Hsiu Hu, Sheng‐Yan Wu, Chi‐Shiun Chiang, Guan‐Jie Huang, Shang‐Da Yang, Shi‐Wei Chu et al. · 2024 · eLife
- A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplificationKenta Yamauchi, Masato Koike, Hiroyuki Hioki · 2025 · Communications Biology
- Preliminary In Vitro Assessment of Decellularized Porcine Descending Aorta for Clinical PurposesMartina Casarin, Tiago Moderno Fortunato, Saima Jalil Imran, Martina Todesco, Deborah Sandrin, Massimo Marchesan, Gino Gerosa, Filippo Romanato, Andrea Bagno, Fabrizio Dal Moro et al. · 2023 · Journal of Functional Biomaterials
- A multiwell plate approach to increase the sample throughput during tissue clearingFumito Akiyama, Katsuhiko Matsumoto, Katsunari Yamashita, Akio Oishi, Takashi Kitaoka, Hiroki R. Ueda · 2024 · Nature Protocols
- Optimizing tissue clearing and imaging methods for human brain tissueMin Sun Kim, Jang Ho Ahn, Ji Eun Mo, Ha Young Song, Deok-Hyeon Cheon, Seong Ho Yoo, Hyung Jin Choi · 2021 · Journal of International Medical Research
- Immunolabeling-compatible PEGASOS tissue clearing for high-resolution whole mouse brain imagingPan Gao, Matthew Rivera, Xiaoxiao Lin, Todd C. Holmes, Hu Zhao, Xiangmin Xu · 2024 · Frontiers in Neural Circuits
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- MAX: a simple, affordable, and rapid tissue clearing reagent for 3D imaging of wide variety of biological specimensBoram Lee, Ju‐Hyun Lee, Dai Hyun Kim, Eun Sil Kim, Bo Kyoung Seo, Im Joo Rhyu, Woong Sun · 2022 · Scientific Reports
- First application of three‐dimensional light sheet fluorescence microscopy to human testicular tumors: New perspectives in histopathologyDiana Pinkert‐Leetsch, Ahmad Kareem, Simon F. Merz, Marc Teckentrup, Alexander Fichtner, Felix Bremmer, Jeannine Missbach‐Guentner · 2025 · Andrology
- Evaluation of the effects of clearing agents, fixation, and process durations on cardiovascular tissue imaging with second harmonic generation and multi-photon modalitiesMaedeh Makki, Zachary A. Molander, Sergio A. Pineda‐Castillo, Devin W. Laurence, Shubhra Singhal, Yasmin Eltwafsha, Gerhard A. Holzapfel, Tingting Gu, Chung‐Hao Lee · 2025 · Frontiers in Bioengineering and Biotechnology
- Advances in tissue optical clearing for 3D imaging in large animalYating Deng, Jianyi Xu, Tingting Yu, Dan Yan Zhu · 2025 · Frontiers of Optoelectronics
- Whole-Kidney Three-Dimensional Staining with CUBICSho Hasegawa, Masaomi Nangaku · 2022 · Journal of Visualized Experiments
- A robust vessel-labeling pipeline with high tissue clearing compatibility for 3D mapping of vascular networksYating Deng, Jingtan Zhu, Xiaomei Liu, Junyao Dai, Tingting Yu, Dan Zhu · 2024 · iScience
- Comprehensive evaluation and application of tissue clearing techniques for 3-D visualization of splenic neural and immune architectureJianing Li, Letian He, Wenling Wang, Siyu Wang, Dan Zhang, Liyun Liang, Guang-Ping Song, Yijian Zhang, Shaoqing Yu, Lei Wang et al. · 2025 · American Journal of Physiology-Cell Physiology
- Protocol for 3D-guided sectioning and deep cell phenotyping via light sheet imaging and 2D spatial multiplexingKevin Bigott, Victoria Schoppel, Manuel Martinez-Osuna, Leon Osinski, Marie-Catherine Tiveron, Daniel Barleben, Simon F. Bornemann, Harold Cremer, Christoph Herbel, Andreas Bosio et al. · 2025 · STAR Protocols
- Tissue Optical Clearing for Biomedical Imaging: From In Vitro to In VivoTingting Yu, Dongyu Li, Dan Yan Zhu · 2021 · Advances in experimental medicine and biology
- The Blood‐Brain Barrier in Both Humans and Rats: A Perspective From 3D ImagingAiwen Chen, Gavin Volpato, Alice Pong, Emma C. Schofield, Jun Huang, Zizhao Qiu, George Paxinos, Huazheng Liang · 2024 · International Journal of Biomedical Imaging