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Cited by 527

The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.

Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection

Open access
Wei, Jin, Alfajaro, Mia Madel, DeWeirdt, Peter C., Hanna, Ruth E., Lu-Culligan, William J., Cai, Wesley L., Strine, Madison S., Zhang, Shang-Min, Graziano, Vincent R., Schmitz, Cameron O., Chen, Jennifer S., Mankowski, Madeleine C., Filler, Renata B., Ravindra, Neal G., Gasque, Victor, de Miguel, Fernando J., Patil, Ajinkya, Chen, Huacui, Oguntuyo, Kasopefoluwa Y., Abriola, Laura, Surovtseva, Yulia V., Orchard, Robert C., Lee, Benhur, Lindenbach, Brett D., Politi, Katerina, van Dijk, David, Kadoch, Cigall, Simon, Matthew D., Yan, Qin, Doench, John G., Wilen, Craig B.·2021·Cell
DOI 10.1016/j.cell.2020.10.028
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Abstract

Identification of host genes essential for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection may reveal novel therapeutic targets and inform our understanding of coronavirus disease 2019 (COVID-19) pathogenesis. Here we performed genome-wide CRISPR screens in Vero-E6 cells with SARS-CoV-2, Middle East respiratory syndrome CoV (MERS-CoV), bat CoV HKU5 expressing the SARS-CoV-1 spike, and vesicular stomatitis virus (VSV) expressing the SARS-CoV-2 spike. We identified known SARS-CoV-2 host factors, including the receptor ACE2 and protease Cathepsin L. We additionally discovered pro-viral genes and pathways, including HMGB1 and the SWI/SNF chromatin remodeling complex, that are SARS lineage and pan-coronavirus specific, respectively. We show that HMGB1 regulates ACE2 expression and is critical for entry of SARS-CoV-2, SARS-CoV-1, and NL63. We also show that small-molecule antagonists of identified gene products inhibited SARS-CoV-2 infection in monkey and human cells, demonstrating the conserved role of these genetic hits across species. This identifies potential therapeutic targets for SARS-CoV-2 and reveals SARS lineage-specific and pan-CoV host factors that regulate susceptibility to highly pathogenic CoVs.

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Metadata

Authors
Wei, J., Alfajaro, M. M., DeWeirdt, P. C., Hanna, R. E., Lu-Culligan, W. J., Cai, W. L., Strine, M. S., Zhang, S.-M., Graziano, V. R., Schmitz, C. O., Chen, J. S., Mankowski, M. C., Filler, R. B., Ravindra, N. G., Gasque, V., de Miguel, F. J., Patil, A., Chen, H., Oguntuyo, K. Y., Abriola, L., Surovtseva, Y. V., Orchard, R. C., Lee, B., Lindenbach, B. D., Politi, K., van Dijk, D., Kadoch, C., Simon, M. D., Yan, Q., Doench, J. G., & Wilen, C. B.
Venue
Cell
Publisher
Elsevier BV
Published
2021-01
Volume
184
Issue
1
Pages
76-91.e13
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2020.10.028
OpenAlex
W3094597057
Cited by
527
References
21
FWCI
11.35
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. https://app.cochat.ai/papers/10.1016/j.cell.2020.10.028
The paper
Wei, J., Alfajaro, M. M., DeWeirdt, P. C., Hanna, R. E., Lu-Culligan, W. J., Cai, W. L., Strine, M. S., Zhang, S.-M., Graziano, V. R., Schmitz, C. O., Chen, J. S., Mankowski, M. C., Filler, R. B., Ravindra, N. G., Gasque, V., de Miguel, F. J., Patil, A., Chen, H., Oguntuyo, K. Y., . . . Wilen, C. B. (2021). Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection. Cell. https://doi.org/10.1016/j.cell.2020.10.028

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.