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Cited by 33Photosynthetic Processes and MechanismsAlgal biology and biofuel productionLight effects on plants

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Systematic identification and characterization of genes in the regulation and biogenesis of photosynthetic machinery

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Kafri, Moshe, Patena, Weronika, Martin, Lance, Wang, Lianyong, Gomer, Gillian, Ergun, Sabrina L., Sirkejyan, Arthur K., Goh, Audrey, Wilson, Alexandra T., Gavrilenko, Sophia E., Breker, Michal, Roichman, Asael, McWhite, Claire D., Rabinowitz, Joshua D., Cross, Frederick R., Wühr, Martin, Jonikas, Martin C.·2023·Cell
DOI 10.1016/j.cell.2023.11.007PMC PMC10760936

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Abstract

Photosynthesis is central to food production and the Earth's biogeochemistry, yet the molecular basis for its regulation remains poorly understood. Here, using high-throughput genetics in the model eukaryotic alga Chlamydomonas reinhardtii , we identify with high confidence (false discovery rate [FDR] < 0.11) 70 poorly characterized genes required for photosynthesis. We then enable the functional characterization of these genes by providing a resource of proteomes of mutant strains, each lacking one of these genes. The data allow assignment of 34 genes to the biogenesis or regulation of one or more specific photosynthetic complexes. Further analysis uncovers biogenesis/regulatory roles for at least seven proteins, including five photosystem I mRNA maturation factors, the chloroplast translation factor MTF1, and the master regulator PMR1, which regulates chloroplast genes via nuclear-expressed factors. Our work provides a rich resource identifying regulatory and functional genes and placing them into pathways, thereby opening the door to a system-level understanding of photosynthesis.

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Metadata

Authors
Kafri, M., Patena, W., Martin, L., Wang, L., Gomer, G., Ergun, S. L., Sirkejyan, A. K., Goh, A., Wilson, A. T., Gavrilenko, S. E., Breker, M., Roichman, A., McWhite, C. D., Rabinowitz, J. D., Cross, F. R., Wühr, M., & Jonikas, M. C.
Affiliations
  • Princeton University (US)
  • Howard Hughes Medical Institute (US)
  • Rockefeller University (US)
Corresponding author
Jonikas, Martin C.
ORCID
Venue
Cell
Publisher
Elsevier BV
Published
2023-12
Volume
186
Issue
25
Pages
5638-5655.e25
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2023.11.007
PMC
PMC10760936
OpenAlex
W4389427712
Cited by
33
References
3
Citations by year
2026: 9 · 2025: 14 · 2024: 10
FWCI
2.94
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion, cc-by)
Funders
  • Princeton University
  • Simons Foundation (55108535)
  • Human Frontier Science Program
  • National Institutes of Health (R35GM128813)
  • European Molecular Biology Organization (ALTF 1006-2017)
  • Howard Hughes Medical Institute
  • National Science Foundation (MCB-1914989)
  • Horowitz Foundation for Social Policy
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
Keywords
photosynthesis, photosynthesis regulation, Chlamydomonas reinhardtii, gene identification, MTF-1, PMR1, photosynthetic complexes
MeSH terms
Chloroplasts, Gene Expression Regulation, Mutation, Photosynthesis, Proteins, Ribosomes, RNA, Messenger, Chlamydomonas reinhardtii

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