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Cited by 269Microtubule and mitosis dynamicsCellular transport and secretionAutophagy in Disease and Therapy

BORC functions upstream of kinesins 1 and 3 to coordinate regional movement of lysosomes along different microtubule tracks

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Guardia, Carlos M., Farías, Ginny G., Jia, Rui, Pu, Jing, Bonifacino, Juan S.·2016·Cell Reports
DOI 10.1016/j.celrep.2016.10.062PMC PMC5136296
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Abstract

The multiple functions of lysosomes are critically dependent on their ability to undergo bidirectional movement along microtubules between the center and the periphery of the cell. Centrifugal and centripetal movement of lysosomes is mediated by kinesin and dynein motors, respectively. We recently described a multi-subunit complex named BORC that recruits the small GTPase Arl8 to lysosomes to promote their kinesin-dependent movement toward the cell periphery. Here, we show that BORC and Arl8 function upstream of two structurally distinct kinesin types: kinesin-1 (KIF5B) and kinesin-3 (KIF1Bβ and KIF1A). Remarkably, KIF5B preferentially moves lysosomes on perinuclear tracks enriched in acetylated α-tubulin, whereas KIF1Bβ and KIF1A drive lysosome movement on more rectilinear, peripheral tracks enriched in tyrosinated α-tubulin. These findings establish BORC as a master regulator of lysosome positioning through coupling to different kinesins and microtubule tracks. Common regulation by BORC enables coordinate control of lysosome movement in different regions of the cell.

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Cited by

Sorted by citations

Authors
Guardia, C. M., Farías, G. G., Jia, R., Pu, J., & Bonifacino, J. S.
Affiliations
National Institutes of Health (US)
Corresponding author
Bonifacino, Juan S.
ORCID
Venue
Cell Reports
Publisher
Elsevier BV
Published
2016-11
Volume
17
Issue
8
Pages
1950-1961
ISSN
2211-1247 (ISSN-L)
DOI
10.1016/j.celrep.2016.10.062
PMC
PMC5136296
OpenAlex
W2556134275
Cited by
269
References
4
Citations by year
2026: 24 · 2025: 23 · 2024: 46 · 2023: 31 · 2022: 32 · 2021: 28 · 2020: 28 · 2019: 21 · 2018: 16 · 2017: 19 · 2016: 1
FWCI
10.86
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell Reports (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • NICHD
  • NIH (ZIA HD001607)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords
lysosome transport, kinesin-1, kinesin-3, KIF5B, KIF1A, tubulin acetylation, tubulin tyrosination, dynein, lysosome positioning, bidirectional transport, small GTPases
MeSH terms
Biological Transport, Cell Membrane, Dyneins, HeLa Cells, Humans, Lysosomes, Microtubules, Models, Biological, Mutation, Kinesins, Multiprotein Complexes

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