Cited by 654Microtubule and mitosis dynamicsCellular transport and secretionAutophagy in Disease and Therapy

Targeted disruption of mouse conventional kinesin heavy chain, kif5B, results in abnormal perinuclear clustering of mitochondria

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Tanaka, Yosuke, Kanai, Yoshimitsu, Okada, Yasushi, Nonaka, Shigenori, Takeda, Sen, Harada, Akihiro, Hirokawa, Nobutaka·1998·Cell

Abstract

Mouse kif5B gene was disrupted by homologous recombination. kif5B-/- mice were embryonic lethal with a severe growth retardation at 9.5-11.5 days postcoitum. To analyze the significance of this conventional kinesin heavy chain in organelle transport, we studied the distribution of major organelles in the extraembryonic cells. The null mutant cells impaired lysosomal dispersion, while brefeldin A could normally induce the breakdown of their Golgi apparatus. More prominently, their mitochondria abnormally clustered in the perinuclear region. This mitochondrial phenotype was reversed by an exogenous expression of KIF5B, and a subcellular fractionation revealed that KIF5B is associated with mitochondria. These data collectively indicate that kinesin is essential for mitochondrial and lysosomal dispersion rather than for the Golgi-to-ER traffic in these cells.

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Crossref · 10.1016/s0092-8674(00)81459-2

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Authors
Tanaka, Y., Kanai, Y., Okada, Y., Nonaka, S., Takeda, S., Harada, A., & Hirokawa, N.
Affiliations
The University of Tokyo (JP)
Corresponding author
Hirokawa, Nobutaka
Venue
Cell
Publisher
Elsevier BV
Published
1998-06
Volume
93
Issue
7
Pages
1147-1158
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2095841601
Cited by
654
References
81
Citations by year
2026: 12 · 2025: 13 · 2024: 24 · 2023: 13 · 2022: 24 · 2021: 27 · 2020: 25 · 2019: 23 · 2018: 24 · 2017: 27 · 2016: 34 · 2015: 24 · 2014: 22 · 2013: 27 · 2012: 31
FWCI
9.24
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Repositories DataBase (IRDB) (submittedVersion)
Funders
Ministry of Education, Culture, Sports, Science and Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords
KIF5B, mitochondrial clustering, organelle transport, Golgi apparatus, brefeldin A, extraembryonic cells, embryonic lethality
MeSH terms
Animals, Anti-Bacterial Agents, Cell Fractionation, Cell Nucleus, Cells, Cultured, Cyclopentanes, Genes, Lethal, Golgi Apparatus, Lysosomes, Microtubules, Mitochondria, Phenotype, RNA, Messenger, Yolk Sac, Nocodazole, Kinesins, Mice, Knockout, Gene Expression Regulation, Developmental, Macrolides, Brefeldin A, Mice

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