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ULK1 Phosphorylates and Regulates Mineralocorticoid Receptor

Shibata, Shigeru, Ishizawa, Kenichi, Wang, Qin, Xu, Ning, Fujita, Toshiro, Uchida, Shunya, Lifton, Richard P.·2018·Cell Reports
DOI 10.1016/j.celrep.2018.06.072
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Abstract

Mineralocorticoid receptor (MR) signaling regulates both renal Na-Cl reabsorption and K + excretion. We previously demonstrated that phosphorylation of S843 in the MR ligand-binding domain in renal intercalated cells is involved in the balance of these activities by regulating ligand binding and signaling. However, the kinase that phosphorylates MR S843 is unknown. Using a high-throughput screen assay of 197 kinases, we found that ULK1 is the principal kinase that is responsible for the phosphorylation of MR S843 . The results were confirmed by in vitro kinase assay, mass spectrometry, and siRNA knockdown experiments. Notably, phosphorylation at MR S843 was markedly reduced in ULK1/2 double knockout mouse embryonic fibroblasts. Upstream, we show that ULK1 activity is inhibited by phosphorylation induced by angiotensin II via mTOR in cell culture and in vivo. These findings implicate mTOR and ULK1 as regulators of MR activity in intercalated cells, a pathway that is critical for maintaining electrolyte homeostasis.

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Research works printed but not linked: 25

  1. Alexander, 2011, Spatial exclusivity combined with positive and negative selection of phosphorylation motifs is the basis for context-dependent mitotic signaling, Sci. Signal., 4, ra42, 10.1126/scisignal.2001796 · DOI
  2. Castañeda-Bueno, 2017, Phosphorylation by PKC and PKA regulate the kinase activity and downstream signaling of WNK4, Proc. Natl. Acad. Sci. USA, 114, E879, 10.1073/pnas.1620315114 · DOI
  3. Egan, 2015, Small molecule inhibition of the autophagy kinase ULK1 and identification of ULK1 substrates, Mol. Cell, 59, 285, 10.1016/j.molcel.2015.05.031 · DOI · Paper page
  4. Eguchi, 1999, Intracellular signaling of angiotensin II-induced p70 S6 kinase phosphorylation at Ser(411) in vascular smooth muscle cells. Possible requirement of epidermal growth factor receptor, Ras, extracellular signal-regulated kinase, and Akt, J. Biol. Chem., 274, 36843, 10.1074/jbc.274.52.36843 · DOI
  5. Gallolu Kankanamalage, 2016, Multistep regulation of autophagy by WNK1, Proc. Natl. Acad. Sci. USA, 113, 14342, 10.1073/pnas.1617649113 · DOI
  6. Gleason, 2015, mTORC2 regulates renal tubule sodium uptake by promoting ENaC activity, J. Clin. Invest., 125, 117, 10.1172/JCI73935 · DOI
  7. Grahammer, 2016, mTORC2 critically regulates renal potassium handling, J. Clin. Invest., 126, 1773, 10.1172/JCI80304 · DOI
  8. Hong, 2008, mTOR-raptor binds and activates SGK1 to regulate p27 phosphorylation, Mol. Cell, 30, 701, 10.1016/j.molcel.2008.04.027 · DOI
  9. Joo, 2011, Hsp90-Cdc37 chaperone complex regulates Ulk1- and Atg13-mediated mitophagy, Mol. Cell, 43, 572, 10.1016/j.molcel.2011.06.018 · DOI · Paper page
  10. Joo, 2016, The noncanonical role of ULK/ATG1 in ER-to-Golgi trafficking is essential for cellular homeostasis, Mol. Cell, 62, 982, 10.1016/j.molcel.2016.05.030 · DOI
  11. Jung, 2011, ULK1 inhibits the kinase activity of mTORC1 and cell proliferation, Autophagy, 7, 1212, 10.4161/auto.7.10.16660 · DOI
  12. Kang, 2013, mTORC1 phosphorylation sites encode their sensitivity to starvation and rapamycin, Science, 341, 1236566, 10.1126/science.1236566 · DOI
  13. Kim, 2011, AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1, Nat. Cell Biol., 13, 132, 10.1038/ncb2152 · DOI · Paper page
  14. Lee, 2015, Deep sequencing in microdissected renal tubules identifies segment-specific transcriptomes, J. Am. Soc. Nephrol., 26, 2669, 10.1681/ASN.2014111067 · DOI
  15. Li, 2016, ULK1/2 constitute a bifurcate node controlling glucose metabolic fluxes in addition to autophagy, Mol. Cell, 62, 359, 10.1016/j.molcel.2016.04.009 · DOI
  16. Mangelsdorf, 1995, The nuclear receptor superfamily: the second decade, Cell, 83, 835, 10.1016/0092-8674(95)90199-X · DOI · Paper page
  17. McAlpine, 2013, Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2, Autophagy, 9, 361, 10.4161/auto.23066 · DOI
  18. Rennefahrt, 2007, Specificity profiling of Pak kinases allows identification of novel phosphorylation sites, J. Biol. Chem., 282, 15667, 10.1074/jbc.M700253200 · DOI
  19. Sengupta, 2013, Regulation of OSR1 and the sodium, potassium, two chloride cotransporter by convergent signals, Proc. Natl. Acad. Sci. USA, 110, 18826, 10.1073/pnas.1318676110 · DOI
  20. Shibata, 2013, Mineralocorticoid receptor phosphorylation regulates ligand binding and renal response to volume depletion and hyperkalemia, Cell Metab., 18, 660, 10.1016/j.cmet.2013.10.005 · DOI
  21. Shibata, 2013, Kelch-like 3 and Cullin 3 regulate electrolyte homeostasis via ubiquitination and degradation of WNK4, Proc. Natl. Acad. Sci. USA, 110, 7838, 10.1073/pnas.1304592110 · DOI
  22. Shibata, 2014, Angiotensin II signaling via protein kinase C phosphorylates Kelch-like 3, preventing WNK4 degradation, Proc. Natl. Acad. Sci. USA, 111, 15556, 10.1073/pnas.1418342111 · DOI
  23. Soulat, 2008, The DEAD-box helicase DDX3X is a critical component of the TANK-binding kinase 1-dependent innate immune response, EMBO J., 27, 2135, 10.1038/emboj.2008.126 · DOI
  24. Verlander, 2003, Deoxycorticosterone upregulates PDS (Slc26a4) in mouse kidney: role of pendrin in mineralocorticoid-induced hypertension, Hypertension, 42, 356, 10.1161/01.HYP.0000088321.67254.B7 · DOI
  25. Wang, 2013, The orphan receptor TR3 participates in angiotensin II-induced cardiac hypertrophy by controlling mTOR signalling, EMBO Mol. Med., 5, 137, 10.1002/emmm.201201369 · DOI

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Metadata

Authors
Shibata, S., Ishizawa, K., Wang, Q., Xu, N., Fujita, T., Uchida, S., & Lifton, R. P.
ORCID
Shibata, Shigeru
Venue
Cell Reports
Publisher
Elsevier BV
Published
2018-07
Volume
24
Issue
3
Pages
569-576
ISSN
2211-1247
DOI
10.1016/j.celrep.2018.06.072
Cited by
46
References
2
Language
en
Licence
Funders
  • Japan Society for the Promotion of Science (JSPS) KAKENHI (15H04837, 15H02538)
  • Kanae Foundation for the Promotion of Medical Science
  • Suzuki Memorial Foundation
  • Takeda Science Foundation
  • NIH (P01DK17433)

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