The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
Neutrophils Obstructing Brain Capillaries Are a Major Cause of No-Reflow in Ischemic Stroke
Open accessAbstract
Despite successful clot retrieval in large vessel occlusion stroke, ∼50% of patients have an unfavorable clinical outcome. The mechanisms underlying this functional reperfusion failure remain unknown, and therapeutic options are lacking. In the thrombin-model of middle cerebral artery (MCA) stroke in mice, we show that, despite successful thrombolytic recanalization of the proximal MCA, cortical blood flow does not fully recover. Using in vivo two-photon imaging, we demonstrate that this is due to microvascular obstruction of ∼20%-30% of capillaries in the infarct core and penumbra by neutrophils adhering to distal capillary segments. Depletion of circulating neutrophils using an anti-Ly6G antibody restores microvascular perfusion without increasing the rate of hemorrhagic complications. Strikingly, infarct size and functional deficits are smaller in mice treated with anti-Ly6G. Thus, we propose neutrophil stalling of brain capillaries to contribute to reperfusion failure, which offers promising therapeutic avenues for ischemic stroke.
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Heart Disease and Stroke Statistics—2019 Update: A Report From the American Heart Association
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Capillary pericytes regulate cerebral blood flow in health and disease
Hall, C. N., Reynell, C., Gesslein, B., Hamilton, N. B., Mishra, A., Sutherland, B. A., O’Farrell, F. M., Buchan, A. M., Lauritzen, M., & Attwell, D. (2014) · Nature · doi:10.1038/nature13165 · Cited by 1,870
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Cerebral ischemia. II. The no-reflow phenomenon.
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Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice
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Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window
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Cited by
Ramos-Cejudo, J., Johnson, A. D., Beiser, A., Seshadri, S., Salinas, J., Berger, J. S., Fillmore, N. R., Do, N., Zheng, C., Kovbasyuk, Z., et al. (2021) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2021.773984 · Cited by 49
Metadata
- Authors
- El Amki, M., Glück, C., Binder, N., Middleham, W., Wyss, M. T., Weiss, T., Meister, H., Luft, A., Weller, M., Weber, B., & Wegener, S.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2020-10
- Volume
- 33
- Issue
- 2
- Pages
- 108260
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W3093119237
- Cited by
- 299
- References
- 46
- FWCI
- 18.43
- Type
- journal article
- Language
- en
- Open access
- gold
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- CoChat. (2026). Citation check: Neutrophils Obstructing Brain Capillaries Are a Major Cause of No-Reflow in Ischemic Stroke. https://app.cochat.ai/papers/10.1016/j.celrep.2020.108260
- The paper
- El Amki, M., Glück, C., Binder, N., Middleham, W., Wyss, M. T., Weiss, T., Meister, H., Luft, A., Weller, M., Weber, B., & Wegener, S. (2020). Neutrophils Obstructing Brain Capillaries Are a Major Cause of No-Reflow in Ischemic Stroke. Cell Reports. https://doi.org/10.1016/j.celrep.2020.108260
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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