A joint-ParB interface promotes Smc DNA recruitment
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Abstract
Chromosomes readily unlink and segregate to daughter cells during cell division, highlighting a remarkable ability of cells to organize long DNA molecules. SMC complexes promote DNA organization by loop extrusion. In most bacteria, chromosome folding initiates at dedicated start sites marked by the ParB/parS partition complexes. Whether SMC complexes recognize a specific DNA structure in the partition complex or a protein component is unclear. By replacing genes in Bacillus subtilis with orthologous sequences from Streptococcus pneumoniae, we show that the three subunits of the bacterial Smc complex together with the ParB protein form a functional module that can organize and segregate foreign chromosomes. Using chimeric proteins and chemical cross-linking, we find that ParB directly binds the Smc subunit. We map an interface to the Smc joint and the ParB CTP-binding domain. Structure prediction indicates how the ParB clamp presents DNA to the Smc complex, presumably to initiate DNA loop extrusion.
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Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764 · see citing works
ColabFold - Making protein folding accessible to all
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Protein complex prediction with AlphaFold-Multimer
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DNA loop extrusion by human cohesin
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- Authors
- Bock, F. P., Liu, H. W., Anchimiuk, A., Diebold-Durand, M.-L., & Gruber, S.
- Affiliations
- University of Lausanne (CH)
- Corresponding author
- Gruber, Stephan
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2022-08
- Volume
- 40
- Issue
- 9
- Pages
- 111273
- ISSN
- 2211-1247 (ISSN-L)
- PMC
- PMC9449133
- OpenAlex
- W4293574562
- Cited by
- 28
- References
- 6
- Citations by year
- 2026: 7 · 2025: 5 · 2024: 7 · 2023: 6 · 2022: 3
- FWCI
- 3.17
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- SERVAL (Université de Lausanne) (publishedVersion, cc-by-nc-nd)
- IRIS (submittedVersion, cc-by-nc-nd)
- HAL (submittedVersion)
- Funders
- European Research Council (724482)
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (197770)
- European Molecular Biology Organization (ALTF 490-2021)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- SMC complex, ParB, bacterial chromosome segregation, chromosome organization, loop extrusion, protein-protein interfaces, Bacillus subtilis, Streptococcus pneumoniae, fusion protein, crosslinking
- MeSH terms
- Bacillus subtilis, Bacterial Proteins, Chromosomes, Bacterial, DNA, DNA, Bacterial, Streptococcus pneumoniae, Cell Cycle Proteins, Chromosome Segregation
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