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Cited by 14DNA and Nucleic Acid ChemistryAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanisms

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Sequencing Mirror-Image DNA Chemically

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Liu, Xianyu, Zhu, Ting F.·2018·Cell Chemical Biology
DOI 10.1016/j.chembiol.2018.06.005
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Abstract

The development of mirror-image biology systems faces a crucial barrier of lacking an L-DNA sequencing technique. Here, we developed a practical method for sequencing mirror-image DNA by adopting the Maxam-Gilbert sequencing approach, through which specific nucleobases in an end-labeled L-DNA are cleaved by achiral chemicals. This technique may facilitate the therapeutic application of nuclease-resistant L-aptamer drugs, and bring the vision of building an alternative, mirror-image self-replicating system closer to reality.

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Crossref · 10.1016/j.chembiol.2018.06.005

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  • l-DNA is limited by the ability to sequence this mirror image DNA.

    “l-DNA is attracting interest for orthogonal data storage47 and as biostable aptamers48−50 but are limited by the ability to sequence this mirror image DNA.49,51”

    From Sequencing of d/l-DNA and XNA by Templated-Synthesis (2025) · cites this paper as [51]

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References 26 · Cited by 14

Research works printed but not linked: 24

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Metadata

Authors
Liu, X., & Zhu, T. F.
Affiliations
Center for Life Sciences (CN)
Corresponding author
Zhu, Ting F.
ORCID
Venue
Cell Chemical Biology
Publisher
Elsevier BV
Published
2018-09
Volume
25
Issue
9
Pages
1151-1156.e3
ISSN
  • 2451-9448 (ISSN-L)
  • 2451-9456
DOI
10.1016/j.chembiol.2018.06.005
OpenAlex
W2883456675
Cited by
14
References
26
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 2
FWCI
0.53
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell chemical biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (21750005, 31470532, 91543102, 31711530153)
  • Ministry of Science and Technology of China (2015CB553402)
  • Beijing Nova Program (Z171100001117011)
  • Tsinghua University Initiative Scientific Research Program (20161080152)
  • Tsinghua University-Peking University Center for Life Sciences
  • Beijing Advanced Innovation Center for Structural Biology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
Maxam-Gilbert sequencing, nuclease resistance
MeSH terms
Base Sequence, DNA, Stereoisomerism, Sequence Analysis, DNA, Aptamers, Nucleotide

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