Effects of fifteen PBDE metabolites, DE71, DE79 and TBBPA on steroidogenesis in the H295R cell line

Song, Renfang; He, Yuhe; Murphy, Margaret B.; Yeung, Leo W.Y.; Yu, Richard M.K.; Lam, Michael H.W.; Lam, Paul K.S.; Hecker, Markus; Giesy, John P.; Wu, Rudolf S.S.; Zhang, Wenbing; Sheng, Guoying; Fu, Jiamo

doi:10.1016/j.chemosphere.2008.01.032

Cited by 79Toxic Organic Pollutants ImpactEffects and risks of endocrine disrupting chemicalsPer- and polyfluoroalkyl substances research

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Effects of fifteen PBDE metabolites, DE71, DE79 and TBBPA on steroidogenesis in the H295R cell line

Song, Renfang, He, Yuhe, Murphy, Margaret B., Yeung, Leo W.Y., Yu, Richard M.K., Lam, Michael H.W., Lam, Paul K.S., Hecker, Markus, Giesy, John P., Wu, Rudolf S.S., Zhang, Wenbing, Sheng, Guoying, Fu, Jiamo·2008·Chemosphere

DOI 10.1016/j.chemosphere.2008.01.032

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Abstract

Polybrominated diphenyl ethers (PBDEs) and tetrabromobisphenol A (TBBPA) are brominated flame retardants that are produced in large quantities and are commonly used in construction materials, textiles, and as polymers in electronic equipment. Environmental and human levels of PBDEs have been increasing in the past 30 years, but the toxicity of PBDEs is not fully understood. Studies on their effects are relatively limited, and show that PBDEs are neurotoxins and potential endocrine disrupters. Hydroxylated (OH) and methoxylated (MeO) PBDEs have also been reported in the adipose tissue, blood and milk of wild animals and humans. In the present study, 15 PBDE metabolites, two BDE mixtures (DE71 and DE79), and TBBPA were studied individually to determine their effects on ten steroidogenic genes, aromatase activity, and concentrations of two steroid hormones (testosterone and 17beta-estradiol) in the H295R human adrenocortical carcinoma cell line. Exposure to 0.05 microM 2'-OH-BDE-68 significantly induced the expression of CYP11A, CYP11B2, CYP17, CYP21, 3betaHSD2, 17betaHSD1, and 17betaHSD4, and the expression of StAR was induced by 6-OH-BDE-90 at the three exposure concentrations. Exposure to DE71 and DE79 resulted in dose-dependent trend towards induction, but these effects were not significant. Exposure to 0.5 microM 2-OH-BDE-123 and 2-MeO-BDE-123 resulted in significantly greater aromatase activity. However, none of the compounds affected sex hormone production at the concentrations tested. Generally, OH-BDEs had a much stronger ability to affect steroidogenic gene expression than MeO-BDEs.

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Authors: Song, R., He, Y., Murphy, M. B., Yeung, L. W. Y., Yu, R. M. K., Lam, M. H. W., Lam, P. K. S., Hecker, M., Giesy, J. P., Wu, R. S. S., Zhang, W., Sheng, G., & Fu, J.
Affiliations: City University of Hong Kong (HK)
University of Saskatchewan (CA)
Chinese Academy of Sciences (CN)
Corresponding author: Lam, Paul K.S.
ORCID: He, Yuhe
Yeung, Leo W.Y.
Yu, Richard M.K.
Lam, Michael H.W.
Lam, Paul K.S.
Hecker, Markus
Giesy, John P.
Wu, Rudolf S.S.
Zhang, Wenbing
Venue: Chemosphere
Publisher: Elsevier BV
Published: 2008-05
Volume: 71
Issue: 10
Pages: 1888-1894
ISSN: 0045-6535 (ISSN-L)
DOI: 10.1016/j.chemosphere.2008.01.032
OpenAlex: W2118679271
Cited by: 79
References: 42
Citations by year: 2025: 1 · 2024: 4 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 1 · 2019: 4 · 2018: 3 · 2017: 4 · 2016: 4 · 2015: 8 · 2014: 6 · 2013: 5 · 2012: 7
FWCI: 5.71
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Chemosphere (publishedVersion)
PubMed (publishedVersion)
The HKU Scholars Hub (University of Hong Kong) (submittedVersion)
Topics: Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Toxic Organic Pollutants Impact
Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
Physical Sciences › Environmental Science › Environmental Chemistry › Per- and polyfluoroalkyl substances research
Keywords: polybrominated diphenyl ethers, PBDE metabolites, tetrabromobisphenol A, steroidogenesis, steroidogenic gene expression, aromatase activity, hydroxylated PBDEs, methoxylated PBDEs, steroid hormones, testosterone, estradiol, H295R cells, endocrine disruptors, brominated flame retardants
MeSH terms: Steroidogenic Acute Regulatory Protein, Aromatase, Cell Survival, Cytochrome P-450 Enzyme System, Estradiol, Gene Expression Regulation, Humans, Hydroxysteroid Dehydrogenases, Phenyl Ethers, Phosphoproteins, Polybrominated Biphenyls, Testosterone, Cell Line, Tumor

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Effects of fifteen PBDE metabolites, DE71, DE79 and TBBPA on steroidogenesis in the H295R cell line

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