Bisphenol A promotes dendritic morphogenesis of hippocampal neurons through estrogen receptor-mediated ERK1/2 signal pathway
Abstract
Bisphenol A (BPA), an environmental endocrine disruptor, has attracted increasing attention to its adverse effects on brain developmental process. The previous study indicated that BPA rapidly increased motility and density of dendritic filopodia and enhanced the phosphorylation of N-methyl-d-aspartate (NMDA) receptor subunit NR2B in cultured hippocampal neurons within 30min. The purpose of the present study was further to investigate the effects of BPA for 24h on dendritic morphogenesis and the underlying mechanisms. After cultured for 5d in vitro, the hippocampal neurons from 24h-old rat were infected by AdV-EGFP to indicate time-lapse imaging of living neurons. The results demonstrated that the exposure of the cultured hippocampal neurons to BPA (10, 100nM) or 17β-estradiol (17β-E2, 10nM) for 24h significantly promoted dendritic development, as evidenced by the increased total length of dendrite and the enhanced motility and density of dendritic filopodia. However, these changes were suppressed by an ERs antagonist, ICI182,780, a non-competitive NMDA receptor antagonist, MK-801, and a mitogen-activated ERK1/2-activating kinase (MEK1/2) inhibitor, U0126. Meanwhile, the increased F-actin (filamentous actin) induced by BPA (100nM) was also completely eliminated by these blockers. Furthermore, the result of western blot analyses showed that, the exposure of the cultures to BPA or 17β-E2 for 24h promoted the expression of Rac1/Cdc42 but inhibited that of RhoA, suggesting Rac1 (…
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Acute exposure to BPA rapidly enhanced short-term passive avoidance memory in developing rats.
“We previously found that acute exposure to BPA rapidly enhanced short-term passive avoidance memory in the developing rats [12].”
From The Rapid Effect of Bisphenol-A on Long-Term Potentiation in Hippocampus Involves Estrogen Receptors and ERK Activation (2017) · cites this paper as [12]
ERK signaling is involved in the chronic effect of BPA on dendritic morphogenesis in hippocampal neurons.
“Our previous studies have demonstrated that ERK signaling is involved not only in the chronic effect of BPA on dendritic morphogenesis in hippocampal neurons but also in the rapid effect of BPA on passive avoidance memory of young rats [12, 23].”
From The Rapid Effect of Bisphenol-A on Long-Term Potentiation in Hippocampus Involves Estrogen Receptors and ERK Activation (2017) · cites this paper as [12]
ERK signaling is involved in the rapid effect of BPA on passive avoidance memory of young rats.
“Our previous studies have demonstrated that ERK signaling is involved not only in the chronic effect of BPA on dendritic morphogenesis in hippocampal neurons but also in the rapid effect of BPA on passive avoidance memory of young rats [12, 23].”
From The Rapid Effect of Bisphenol-A on Long-Term Potentiation in Hippocampus Involves Estrogen Receptors and ERK Activation (2017) · cites this paper as [12]
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Self-citationRapid Estrogen Signaling in the Brain
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Chen, X., Wang, Y., Xu, F., Wei, X., Zhang, J., Wang, C., Wei, H., Xu, S., Yan, P., Zhou, W., et al. (2017) · Neural Plasticity · doi:10.1155/2017/5196958 · Cited by 24 · see citing works
“Our previous study also confirms that E 2 (10 nM) significantly increases the total dendritic length and enhances motility and density of dendritic filopodia in cultured hippocampal neurons [12].”
BackgroundInfluentialSelf-citation
- Authors
- Xu, X., Lu, Y., Zhang, G., Chen, L., Tian, D., Shen, X., Yang, Y., & Dong, F.
- Affiliations
- Zhejiang Normal University (CN)
- Zhejiang University of Science and Technology (CN)
- Corresponding author
- Xu, Xiaohong
- Venue
- Chemosphere
- Publisher
- Elsevier BV
- Published
- 2014-02
- Volume
- 96
- Pages
- 129-137
- ISSN
- 0045-6535 (ISSN-L)
- OpenAlex
- W1966298313
- Cited by
- 49
- References
- 4
- Citations by year
- 2025: 4 · 2024: 4 · 2023: 3 · 2022: 2 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 2 · 2017: 6 · 2016: 7 · 2015: 4 · 2014: 6
- FWCI
- 4.17
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemosphere (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Natural Science Foundation of China (81172627 and 30872087)
- Zhejiang Province Natural Science Foundation (Z2090955)
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Effects and risks of endocrine disrupting chemicals
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- bisphenol A, dendrite morphogenesis, hippocampal neurons, estrogen receptor, ERK signaling, endocrine disruptors, estradiol, filopodia, actin cytoskeleton, Rho GTPases, Rac1, Cdc42, RhoA, NMDA receptor, neuronal development, brain development, fulvestrant, U0126, MK-801, time-lapse imaging
- MeSH terms
- Bisphenol A Compounds, Animals, Benzhydryl Compounds, Dendrites, Estradiol, Female, Hippocampus, Morphogenesis, Neurons, Phenols, Phosphorylation, Receptors, Estrogen, Receptors, N-Methyl-D-Aspartate, MAP Kinase Signaling System, Estrogen Receptor alpha, Rats, Endocrine Disruptors
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