Pediatric reference intervals for 28 chemistries and immunoassays on the Roche cobas® 6000 analyzer—A CALIPER pilot study
Abstract
Objective The aim of this study was to determine age- and sex-specific pediatric reference intervals for 28 analytes on the Roche cobas 6000 analyzer.Design and methods The study was conducted at the Hospital for Sick Children in Toronto, Canada. Approximately 600 outpatient samples from a pediatric population deemed to be metabolically stable were subdivided into five age classes ranging from 0 to 20 years of age and further partitioned by gender. Reference intervals were established, after removal of samples significantly affected by hemolysis, icterus and lipemia and outlier exclusion, using the Robust statistical method to obtain the 2.5th and 97.5th percentiles.Results Age (birth to 20 years of age) and gender-appropriate pediatric reference intervals for 28 analytes are reported.Conclusions These reference intervals provide the basis for clinical interpretation of laboratory results using the Roche cobas 6000 analyzer or related instrumentation/methods, provided adequate reference interval verification studies are performed.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Cross-sectional studies generally show higher TSH upper limits in younger children (<5 years) compared with older children (≥5 years), with a gradual decline in TSH concentration towards adult age (15-18 years), and lower FT3 with higher increases in FT4 concentrations from age 4 years.
“Cross-sectional studies generally show higher TSH upper limits in younger children (<5 years) compared with older children (≥5 years), with a gradual decline in TSH concentration towards adult age (15-18 years), 119-124 and lower FT3 with higher increases in FT4 concentrations from age 4 years.[125][126][127][128]”
From Hypothyroidism (2024) · cites this paper as [126]
Free triiodothyronine (FT3) levels fall with age.
“Free triiodothyronine (FT3) levels fall with age and appear to play a role in pubertal development, during which it shows a strong relationship with fat mass.”
From Age-related variation in thyroid function – a narrative review highlighting important implications for research and clinical practice (2023) · cites this paper as [43]
Previous cross-sectional studies have indicated that FT3 falls and FT4 rises from age 4 years.
“Previous cross-sectional studies have indicated that free triiodothyronine (FT3) falls and FT4 rises from age 4 years [43–45].”
From Age-related variation in thyroid function – a narrative review highlighting important implications for research and clinical practice (2023) · cites this paper as [43]
Cites
Reference intervals: an update
Horn, P. S., & Pesce, A. J. (2003) · Clinica Chimica Acta · doi:10.1016/s0009-8981(03)00133-5 · Cited by 417 · see citing works
A robust approach to reference interval estimation and evaluation
Horn, P. S., Pesce, A. J., & Copeland, B. E. (1998) · Clinical Chemistry · doi:10.1093/clinchem/44.3.622 · Cited by 272 · see citing works
Clinical laboratory reference intervals in pediatrics: The CALIPER initiative
Jung, B., & Adeli, K. (2009) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2009.06.025 · Cited by 103 · see citing works
Self-citationChan, M. K., Seiden‐Long, I., Aytekin, M., Quinn, F. A., Ravalico, T. H., Ambruster, D., & Adeli, K. (2009) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2009.01.014 · Cited by 75 · see citing works
Self-citationThe Origin of Reference Intervals
Friedberg, R. C., Souers, R. J., Wagar, E. A., et al. (2007) · Archives of Pathology & Laboratory Medicine · doi:10.5858/2007-131-348-toori · Cited by 74 · see citing works
Soldin, O. P., Hoffman, E. G., Waring, M. A., et al. (2005) · Clinica Chimica Acta · doi:10.1016/j.cccn.2005.01.006 · Cited by 72 · see citing works
Mansoub, S., Chan, M. K., & Adeli, K. (2006) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2006.02.013 · Cited by 70 · see citing works
Self-citationClosing the gaps in paediatric reference intervals: the CALIPER initiative.
Schnabl, K. L., Chan, M. K., Gong, Y., et al. (2008) · PubMed · Cited by 67 · see citing works
Self-citationSerum CrossLaps: Pediatric Reference Intervals from Birth to 19 Years of Age
Crofton, P. M., Evans, N., Taylor, M. R. H., et al. (2002) · Clinical Chemistry · doi:10.1093/clinchem/48.4.671 · Cited by 63 · see citing works
Pediatric reference intervals for lipids and apolipoproteins on the VITROS 5,1 FS Chemistry System
Yip, P. S. F., Chan, M. K., Nelken, J., Lepage, N., Brotea, G., & Adeli, K. (2006) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2006.06.012 · Cited by 46 · see citing works
Self-citationSoldin, O. P., Dahlin, J. R. B., Gresham, E. G., King, J., & Soldin, S. J. (2008) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2008.04.025 · Cited by 44 · see citing works
Pediatric reference intervals: Critical gap analysis and establishment of a national initiative
Schnabl, K. L., Chan, M. K., & Adeli, K. (2006) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2006.03.009 · Cited by 39 · see citing works
Self-citationLepage, N., Li, D., Kavsak, P. A., Bamforth, F. J., Callahan, J. W., Dooley, K., & Potter, M. (2006) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2006.02.011 · Cited by 35 · see citing works
Reference Interval Computation Using Robust vs Parametric and Nonparametric Analyses
Horn, P. S., Pesce, A. J., & Copeland, B. E. (1999) · Clinical Chemistry · doi:10.1093/clinchem/45.12.2284 · Cited by 35 · see citing works
DELVIN, E. E., Grey, V. L., & Vergee, Z. (2006) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2006.03.006 · Cited by 25 · see citing works
Davis, M. L., Austin, C., Messmer, B., et al. (1996) · Clinical Biochemistry · doi:10.1016/0009-9120(96)00046-x · Cited by 13 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Taylor, P. N., Medici, M. M., Hubalewska-Dydejczyk, A., & Boelaert, K. (2024) · The Lancet · doi:10.1016/s0140-6736(24)01614-3 · Cited by 128 · see citing works
Dathan-Stumpf, A., Vogel, M., Hiemisch, A., Thiery, J., Burkhardt, R., Kratzsch, J., & Kiess, W. (2016) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2016.02.010 · Cited by 75 · see citing works
Taylor, P. N., Lansdown, A., Witczak, J., Khan, R., Rees, A., Dayan, C. M., & Okosieme, O. (2023) · Thyroid Research · doi:10.1186/s13044-023-00149-5 · Cited by 72 · see citing works
Önsesveren, I., Barjaktarovic, M., Chaker, L., de Rijke, Y. B., Jaddoe, V. W. V., van Santen, H. M., Visser, T. J., Peeters, R. P., & Korevaar, T. I. M. (2017) · Thyroid® · doi:10.1089/thy.2017.0262 · Cited by 58 · see citing works
Taylor, P. N., Sayers, A., Okosieme, O., Das, G., Draman, M. S., Tabasum, A., Abusahmin, H., Rahman, M., Stevenson, K., Groom, A., et al. (2017) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2016-3605 · Cited by 34 · see citing works
- Authors
- Kulasingam, V., Jung, B. P., Blasutig, I. M., Baradaran, S., Chan, M. K., Aytekin, M., Colantonio, D. A., & Adeli, K.
- Affiliations
- University of Toronto (CA)
- Children's & Women's Health Centre of British Columbia (CA)
- Hospital for Sick Children (CA)
- Marmara University (TR)
- Corresponding author
- Adeli, Khosrow
- ORCID
- Venue
- Clinical Biochemistry
- Publisher
- Elsevier BV
- Published
- 2010-09
- Volume
- 43
- Issue
- 13-14
- Pages
- 1045-1050
- ISSN
- 0009-9120 (ISSN-L)
- OpenAlex
- W2012985409
- Cited by
- 58
- References
- 16
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 4 · 2023: 3 · 2021: 4 · 2020: 3 · 2019: 4 · 2018: 1 · 2017: 4 · 2016: 3 · 2015: 4 · 2014: 4 · 2013: 5 · 2012: 10
- FWCI
- 4.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Dspace Repository (Marmara Üniversitesi) (submittedVersion)
- Funders
- Hospital for Sick Children
- Topics
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Social Sciences › Decision Sciences › Statistics, Probability and Uncertainty › Scientific Measurement and Uncertainty Evaluation
- Health Sciences › Medicine › Pediatrics, Perinatology and Child Health › Neonatal Health and Biochemistry
- Keywords
- pediatric reference intervals, immunoassay
- MeSH terms
- Adolescent, Blood Chemical Analysis, Canada, Child, Child, Preschool, Female, Humans, Immunoassay, Infant, Infant, Newborn, Male, Pilot Projects, Reference Values, Clinical Laboratory Techniques, Young Adult
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
