The DOI matches its Crossref record; 123 of the 123 references it cites were found in a registry or an index.
LC–MS/MS analysis of steroids in the clinical laboratory
Abstract
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a powerful tool that is changing the way we analyse steroids in the clinical laboratory. It is already opening up the field of steroid analysis in endocrinology and is providing new applications for individual steroids and panels of steroids in different clinical conditions. LC-MS/MS is now well-accepted technology and is increasingly being used to replace problematic immunoassay methods because of greater sensitivity and specificity. Improved sample preparation, modern chromatography methods, and sensitive, faster scanning mass spectrometers have all played a role in improving LC-MS/MS. LC-MS/MS is also playing a key role in improving the quality of assays through the development of reference measurement procedures, characterisation of reference materials and multi-site calibration programmes. There is increasing interest in multiplexing steroid assays into panels of diagnostic tests to aid and improve the diagnosis and monitoring of disease.
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“A range of liquid chromatography tandem mass spectrometry assays for salivary cortisol/cortisone and salivary androgens have thus been developed and implemented in routine clinical laboratories (Keevil, 2016).”
From Intracrine androgen biosynthesis, metabolism and action revisited (2018) · cites this paper as Keevil, 2016
Citations
Cites
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Self-citation
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Cited by
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After another decade: LC–MS/MS became routine in clinical diagnostics
Seger, C., & Salzmann, L. (2020) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2020.03.004 · Cited by 309
11-Oxygenated C19 steroids are the predominant androgens in polycystic ovary syndrome
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Liquid chromatography–tandem mass spectrometry for clinical diagnostics
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Intracrine androgen biosynthesis, metabolism and action revisited
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Metadata
- Authors
- Keevil, B. G.
- Affiliations
- Manchester Academic Health Science Centre (GB)
- Corresponding author
- Keevil, Brian G.
- ORCID
- Keevil, Brian G.
- Venue
- Clinical Biochemistry
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 49
- Issue
- 13-14
- Pages
- 989-997
- ISSN
- 0009-9120 (ISSN-L)
- OpenAlex
- W2344158997
- Cited by
- 149
- References
- 123
- Citations by year
- 2026: 13 · 2025: 6 · 2024: 16 · 2023: 16 · 2022: 23 · 2021: 16 · 2020: 18 · 2019: 17 · 2018: 12 · 2017: 11 · 2016: 1
- FWCI
- 11.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Topics
- Health Sciences › Health Professions › Medical Laboratory Technology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- steroid analysis, LC-MS/MS, laboratory diagnosis, endocrinology, sample preparation, reference measurement procedure, reference materials, multi-site calibration, disease diagnosis and monitoring
- MeSH terms
- Calibration, Chromatography, Liquid, Humans, Hydrocortisone, Laboratories, Steroids, Tandem Mass Spectrometry
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