The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Detection and Quantitation of Circulating Human Irisin by Tandem Mass Spectrometry
Open accessAbstract
SUMMARY Exercise provides many health benefits, including improved metabolism, cardiovascular health, and cognition. We have shown previously that FNDC5, a type I transmembrane protein, and its circulating form irisin, convey some of these benefits in mice. However, recent reports questioned the existence of circulating human irisin both because human FNDC5 has a non-canonical ATA translation start and claims that many human irisin antibodies used in commercial ELISA kits lack required specificity. In this paper we have identified and quantitated human irisin in blood using mass spectrometry with control peptides enriched with stable isotopes as internal standards. This precise state-of-the-art method shows that human irisin is mainly translated from its non-canonical start codon and circulates at ~3.6 ng/ml in sedentary individuals; this level is increased to ~4.3 ng/ml in individuals undergoing aerobic interval training. These data unequivocally demonstrate that human irisin exists, circulates and is regulated by exercise.
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A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis
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Self-citationExercise induces hippocampal BDNF through a PGC-1α/FNDC5 pathway
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Self-citationIntegrative Biology of Exercise
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“However, many reports of human irisin fall below this level (Kraemer et al., 2014; Kurdiova et al., 2014; Moraes et al., 2013; Wang et al., 2015; Zhang et al., 2014).”
Huh, J. Y., Mougios, V., Kabasakalis, A., Fatouros, I., Siopi, A., Douroudos, I. I., Filippaios, A., Panagiotou, G., Park, K. H., & Mantzoros, C. S. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-1437 · Cited by 363
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Exercise-linked FNDC5/irisin rescues synaptic plasticity and memory defects in Alzheimer’s models
Lourenco, M. V., Frozza, R. L., de Freitas, G. B., Zhang, H., Kincheski, G. C., Ribeiro, F. C., Gonçalves, R. A., Clarke, J. R., Beckman, D., Staniszewski, A., et al. (2019) · Nature Medicine · doi:10.1038/s41591-018-0275-4 · Cited by 935
Exercise benefits in cardiovascular disease: beyond attenuation of traditional risk factors
Fiuza-Luces, C., Santos-Lozano, A., Joyner, M., Carrera-Bastos, P., Picazo, O., Zugaza, J. L., Izquierdo, M., Ruilope, L. M., & Lucia, A. (2018) · Nature Reviews Cardiology · doi:10.1038/s41569-018-0065-1 · Cited by 803
Sleiman, S. F., Henry, J., Al-Haddad, R., El Hayek, L., Abou Haidar, E., Stringer, T., Ulja, D., Karuppagounder, S. S., Holson, E. B., Ratan, R. R., et al. (2016) · eLife · doi:10.7554/elife.15092 · Cited by 810
Physical activity and muscle-brain crosstalk
Pedersen, B. K. (2019) · Nature Reviews Endocrinology · doi:10.1038/s41574-019-0174-x · Cited by 772
Targeting adipose tissue in the treatment of obesity-associated diabetes
Kusminski, C. M., Bickel, P. E., & Scherer, P. E. (2016) · Nature Reviews Drug Discovery · doi:10.1038/nrd.2016.75 · Cited by 678
Metadata
- Authors
- Jedrychowski, M. P., Wrann, C. D., Paulo, J. A., Gerber, K. K., Szpyt, J., Robinson, M. M., Nair, K. S., Gygi, S. P., & Spiegelman, B. M.
- Venue
- Cell Metabolism
- Publisher
- Elsevier BV
- Published
- 2015-10
- Volume
- 22
- Issue
- 4
- Pages
- 734-740
- ISSN
- 1550-4131 (ISSN-L)
- OpenAlex
- W2134630622
- Cited by
- 558
- References
- 9
- FWCI
- 39.91
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Jedrychowski, M. P., Wrann, C. D., Paulo, J. A., Gerber, K. K., Szpyt, J., Robinson, M. M., Nair, K. S., Gygi, S. P., & Spiegelman, B. M. (2015). Detection and Quantitation of Circulating Human Irisin by Tandem Mass Spectrometry. Cell Metabolism. https://doi.org/10.1016/j.cmet.2015.08.001
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