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How to: identify non-tuberculous Mycobacterium species using MALDI-TOF mass spectrometry
Open accessAbstract
Background The implementation of MALDI-TOF MS for microorganism identification has changed the routine of the microbiology laboratories as we knew it. Most microorganisms can now be reliably identified within minutes using this inexpensive, user-friendly methodology. However, its application in the identification of mycobacteria isolates has been hampered by the structure of their cell wall. Improvements in the sample processing method and in the available database have proved key factors for the rapid and reliable identification of non-tuberculous mycobacteria isolates using MALDI-TOF MS.Aims The main objective is to provide information about the proceedings for the identification of non-tuberculous isolates using MALDI-TOF MS and to review different sample processing methods, available databases, and the interpretation of the results.Sources Results from relevant studies on the use of the available MALDI-TOF MS instruments, the implementation of innovative sample processing methods, or the implementation of improved databases are discussed.Content Insight about the methodology required for reliable identification of non-tuberculous mycobacteria and its implementation in the microbiology laboratory routine is provided.Implications Microbiology laboratories where MALDI-TOF MS is available can benefit from its capacity to identify most clinically interesting non-tuberculous mycobacteria in a rapid, reliable, and inexpensive manner.
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Bacteria and yeasts, mycobacteria and moulds can now be identified accurately and rapidly by MALDI-TOF MS.
“Bacteria and yeasts (most clinical identifications), mycobacteria136 and moulds137 can now be identified accurately and rapidly.”
From A high-stringency blueprint of the human proteome (2020) · cites this paper as [136]
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References 28 · Cited by 138
Metadata
- Authors
- Alcaide, F., Amlerová, J., Bou, G., Ceyssens, P. J., Coll, P., Corcoran, D., Fangous, M.-S., González-Álvarez, I., Gorton, R., Greub, G., Hery-Arnaud, G., Hrábak, J., Ingebretsen, A., Lucey, B., Marekoviċ, I., Mediavilla-Gradolph, C., Monté, M. R., O'Connor, J., O'Mahony, J., Opota, O., O'Reilly, B., Orth-Höller, D., Oviaño, M., Palacios, J. J., Palop, B., Pranada, A. B., Quiroga, L., Rodríguez-Temporal, D., Ruiz-Serrano, M. J., Tudó, G., Van den Bossche, A., van Ingen, J., & Rodriguez-Sanchez, B.
- Affiliations
- Bellvitge University Hospital (ES)
- Complexo Hospitalario Universitario A Coruña (ES)
- Institut Scientifique de Santé Publique (BE)
- Hospital de Sant Pau (ES)
- Cork University Hospital (IE)
- Hospital Universitario Central de Asturias (ES)
- University of Lausanne (CH)
- Oslo University Hospital (NO)
- Munster Technological University (IE)
- Hospital Regional Universitario de Málaga (ES)
- Hospital Clínic de Barcelona (ES)
- Hospital General Universitario Gregorio Marañón (ES)
- ORCID
- Venue
- Clinical Microbiology and Infection
- Publisher
- Elsevier BV
- Published
- 2018-06
- Volume
- 24
- Issue
- 6
- Pages
- 599-603
- ISSN
- 1198-743X (ISSN-L)
- OpenAlex
- W2770318071
- Cited by
- 138
- References
- 28
- Citations by year
- 2026: 13 · 2025: 18 · 2024: 9 · 2023: 26 · 2022: 18 · 2021: 16 · 2020: 18 · 2019: 12 · 2018: 7
- FWCI
- 1.32
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Clinical Microbiology and Infection (publishedVersion)
- PubMed (publishedVersion)
- SERVAL (Université de Lausanne) (publishedVersion, other-oa)
- IRIS (submittedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- Topics
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- nontuberculous mycobacteria, MALDI-TOF mass spectrometry, mycobacterial identification, sample processing, mycobacterial cell wall, rapid identification
- MeSH terms
- Bacteriological Techniques, Humans, Mycobacterium Infections, Nontuberculous, Nontuberculous Mycobacteria, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Workflow
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