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Cited by 76Advanced Electron Microscopy Techniques and ApplicationsSupramolecular Self-Assembly in MaterialsAdvanced Materials Characterization Techniques

Advances in cryogenic transmission electron microscopy for the characterization of dynamic self-assembling nanostructures

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Newcomb, Christina J., Moyer, Tyson J., Lee, Sungsoo S., Stupp, Samuel I.·2012·Current Opinion in Colloid & Interface Science
DOI 10.1016/j.cocis.2012.09.004PMC PMC3510006
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Abstract

Elucidating the structural information of nanoscale materials in their solvent-exposed state is crucial, as a result, cryogenic transmission electron microscopy (cryo-TEM) has become an increasingly popular technique in the materials science, chemistry, and biology communities. Cryo-TEM provides a method to directly visualize the specimen structure in a solution-state through a thin film of vitrified solvent. This technique complements X-ray, neutron, and light scattering methods that probe the statistical average of all species present; furthermore, cryo-TEM can be used to observe changes in structure over time. In the area of self-assembly, this tool has been particularly powerful for the characterization of natural and synthetic small molecule assemblies, as well as hybrid organic-inorganic composites. In this review, we discuss recent advances in cryogenic TEM in the context of self-assembling systems with emphasis on characterization of transitions observed in response to external stimuli.

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  • Cryo-TEM has demonstrated to be quite interesting for determining the impact of polymer concentration on micelles shape.

    “Cryo-TEM has demonstrated to be quite interesting for determining the impact of polymer concentration on micelles shape.”

    From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [176]

    The Introduction says cryo-TEM can visualize self-assembled structures as concentration varies, and Section 3.2 describes its use to examine block-copolymer micelle shapes. The paper does not establish that the micelle-shape changes it describes were caused by changes in polymer concentration; those examples vary solvent composition, mixing time, or other conditions. Page numbers are unavailable in our copy. (LLM, conf=0.84)

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  • TEM images showed spherical micelles at 50 mg/ml, sphere-to-rod transition after dilution to 25 mg/ml, different micellar morphologies including lamellar sheets, shorter rods and smaller spheres at 2 mg/ml, and only longer rods and spheres at 0.5 mg/ml.

    “TEM images showed micelles with spherical shape at a concentration of 50 mg/ml, sphere-to-rod transition after dilution to 25 mg/ml, different micellar morphologies, including lamellar sheets, shorter rods and smaller spheres at concentration of 2 mg/ml, while at 0.5 mg/ml only longer rods and spheres were visualized (Fig. 8).”

    From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [176]

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  • Fairley et al. used Cryo-TEM to confirm that the morphologies found by TEM for PEG5000-b-PCL4100 actually existed in solution.

    “Fairley et al. [176] studied by TEM the assembling behavior of the amphiphilic copolymer PEG 5000 -b-PCL 4100 at concentration of 50 mg/ml, 25 mg/ml, 2 mg/ml and 0.5 mg/ml and used Cryo-TEM to confirm that the morphologies found by TEM actually existed in solution.”

    From Polymeric micelles in drug delivery: An insight of the techniques for their characterization and assessment in biorelevant conditions (2021) · cites this paper as [176]

    Section 2 (Sample Preparation and Instrumentation; page unavailable) explains that cryo-TEM can image hydrated structures without the drying artifacts of conventional TEM. But our copy does not describe Fairley et al., PEG5000-b-PCL4100, or a comparison confirming that this copolymer’s TEM morphologies existed in solution. Searches for those names and the stated concentrations found no relevant passage. (LLM, conf=0.73)

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Cited by

Sorted by citations

Authors
Newcomb, C. J., Moyer, T. J., Lee, S. S., & Stupp, S. I.
Affiliations
Northwestern University (US)
Corresponding author
Stupp, Samuel I.
ORCID
Stupp, Samuel I.
Venue
Current Opinion in Colloid & Interface Science
Publisher
Elsevier BV
Published
2012-12
Volume
17
Issue
6
Pages
350-359
ISSN
1359-0294 (ISSN-L)
DOI
10.1016/j.cocis.2012.09.004
PMC
PMC3510006
OpenAlex
W1988448289
Cited by
76
References
4
Citations by year
2026: 6 · 2025: 3 · 2024: 6 · 2023: 6 · 2022: 5 · 2021: 13 · 2020: 6 · 2019: 3 · 2018: 7 · 2017: 3 · 2016: 5 · 2015: 4 · 2014: 3 · 2013: 6
FWCI
0.57
Type
journal article
Language
en
Open access
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Current Opinion in Colloid & Interface Science (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (2R01DE015920-06, 2R01EB003806-06A2, 1U54CA151880-01)
  • U.S. Department of Energy
  • Office of Basic Energy Sciences, Division of Materials Sciences and Engineering (DE-FG02-00ER45810)
  • DARPA (W911NF-09-1-0044)
  • National Science Foundation (DMR-1006713)
  • National Science Foundation Graduate Research Fellowship
  • Samsung Scholarship Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
  • Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
  • Physical Sciences › Engineering › Biomedical Engineering › Advanced Materials Characterization Techniques
Keywords
cryo-TEM, self-assembly, vitrification, organic-inorganic hybrid materials, structural dynamics

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