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Cited by 97Bacterial biofilms and quorum sensingCellular Mechanics and InteractionsPolymer Surface Interaction Studies

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Alteration of bacterial adhesion induced by the substrate stiffness

Guégan, C., Garderes, J., Le Pennec, Gaël, Gaillard, F., Fay, F., Linossier, I., Herry, J.-M., Fontaine, M.-N. Bellon, Réhel, K. Vallée·2014·Colloids and Surfaces B: Biointerfaces
DOI 10.1016/j.colsurfb.2013.10.010
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Abstract

The aim of this paper is to study the impact of the substrate stiffness on the bacterial adhesion. For this purpose, agarose hydrogels are used as substrates with controlled mechanical properties. Indeed, the elastic modulus of these hydrogels, more precisely the shear storage moduli G', evolves with the agarose concentration (in this study from 0.75% to 3%). Other physico-chemical characteristics of the surface, known to be involved in bacterial adhesion, as hydrophobicity, were confirmed to remain constant. Two marine bacterial strains, a positive Gram Bacillus sp. 4J6 and a negative Gram Pseudoalteromonas sp. D41 were selected. Their retention on the substrates was analysed by confocal laser scanning microscopy and by counting of viable adhered bacteria. It was demonstrated that surface elastic modulus correlated with bacterial retention. Bacteria D41 adhered in higher numbers to rigid surfaces. For 4J6, bacterial adhesion patterns were changed: clusterings were observed on surfaces with lower elastic modulus. Furthermore, a proteomic study, based on the total soluble proteome of D41 strain, highlights an impact of elastic modulus on proteins synthesis. These data demonstrated an adapted response of adhering bacteria on hydrogels of varying mechanical properties.

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References 34 · Cited by 97

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Metadata

Authors
Guégan, C., Garderes, J., Le Pennec, G., Gaillard, F., Fay, F., Linossier, I., Herry, J.-M., Fontaine, M.-N. B., & Réhel, K. V.
Affiliations
  • Université de Bretagne Sud (FR)
  • Station Biologique de Roscoff (FR)
  • AgroParisTech (FR)
ORCID
Venue
Colloids and Surfaces B: Biointerfaces
Publisher
Elsevier BV
Published
2014-02
Volume
114
Pages
193-200
ISSN
0927-7765 (ISSN-L)
DOI
10.1016/j.colsurfb.2013.10.010
OpenAlex
W1987737799
Cited by
97
References
34
Citations by year
2026: 4 · 2025: 9 · 2024: 11 · 2023: 7 · 2022: 9 · 2021: 5 · 2020: 6 · 2019: 10 · 2018: 11 · 2017: 10 · 2016: 4 · 2015: 5 · 2014: 6
FWCI
2.24
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Colloids and Surfaces B Biointerfaces (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
Institut Français de Recherche pour l'Exploitation de la Mer
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
Keywords
bacterial adhesion, substrate stiffness, agarose gel, elastic modulus, shear storage modulus, hydrogel mechanical properties, marine bacteria, Pseudomonas, Pseudoalteromonas, bacterial clustering, bacterial proteome
MeSH terms
Bacillus, Bacterial Adhesion, Bacterial Proteins, Electrons, Movement, Solubility, Mass Spectrometry, Surface Tension, Colony Count, Microbial, Hydrogels, Pseudoalteromonas, Proteomics, Microbial Viability, Elastic Modulus

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CoChat. (2026). Citation check: Alteration of bacterial adhesion induced by the substrate stiffness. https://app.cochat.ai/papers/10.1016/j.colsurfb.2013.10.010
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Guégan, C., Garderes, J., Le Pennec, G., Gaillard, F., Fay, F., Linossier, I., Herry, J.-M., Fontaine, M.-N. B., & Réhel, K. V. (2014). Alteration of bacterial adhesion induced by the substrate stiffness. Colloids and Surfaces B: Biointerfaces. https://doi.org/10.1016/j.colsurfb.2013.10.010

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