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Improved vitrification solutions based on the predictability of vitrification solution toxicity

Fahy, Gregory M, Wowk, Brian, Wu, Jun, Paynter, Sharon·2004·Cryobiology
DOI 10.1016/j.cryobiol.2003.11.004
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Abstract

Long-term preservation of complex engineered tissues and organs at cryogenic temperatures in the absence of ice has been prevented to date by the difficulty of discovering combinations of cryoprotectants that are both sufficiently non-toxic and sufficiently stable to allow viability to be maintained and ice formation to be avoided during slow cooling to the glass transition temperature and subsequent slow rewarming. A new theory of the origin of non-specific cryoprotectant toxicity was shown to account, in a rabbit renal cortical slice model, for the toxicities of 20 vitrification solutions and to permit the design of new solutions that are dramatically less toxic than previously known solutions for diverse biological systems. Unfertilized mouse ova vitrified with one of the new solutions were successfully fertilized and regained 80% of the absolute control (untreated) rate of development to blastocysts, whereas ova vitrified in VSDP, the best previous solution, developed to blastocysts at a rate only 30% of that of controls. Whole rabbit kidneys perfused at -3 degrees C with another new solution at a concentration of cryoprotectant (8.4M) that was previously 100% lethal at this temperature exhibited no damage after transplantation and immediate contralateral nephrectomy. It appears that cryoprotectant solutions that are composed to be at the minimum concentrations needed for vitrification at moderate cooling rates are toxic in direct proportion to the average strength of wat…

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Metadata

Authors
Fahy, G. M., Wowk, B., Wu, J., & Paynter, S.
Venue
Cryobiology
Publisher
Elsevier BV
Published
2004-02
Volume
48
Issue
1
Pages
22-35
ISSN
0011-2240
DOI
10.1016/j.cryobiol.2003.11.004
Cited by
284
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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