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Fracture formation in vitrified thin films of cryoprotectants

Rabin, Yoed, Steif, Paul S., Hess, Katherine C., Jimenez-Rios, Jorge L., Palastro, Matthew C.·2006·Cryobiology
DOI 10.1016/j.cryobiol.2006.03.013
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Abstract

As a part of an ongoing effort to study the continuum mechanics effects associated with cryopreservation, the current report focuses on fracture formation in vitrified thin films of cryoprotective agents. The current study combines experimental observations with continuum mechanics analysis. Experimental results have been developed using a new imaging device, termed a "cryomacroscope", which has been recently presented by the current research team. A newly developed liquid nitrogen-based cooling stage is presented in this paper. The samples under investigation are 0.5 ml droplets of cryoprotective agents, having a characteristic diameter of 20 mm and a characteristic thickness of 1.5 mm. Tested samples included dimethyl sulfoxide (DMSO) in a concentration range from 6 to 8.4M, and the cryoprotectant cocktails VS55 and DP6. Some samples contained small bovine muscle segments, having a characteristic dimension of 1mm, in order to study stress concentration effects. Experimental results show that the onset of fracturing in vitrified films of cryoprotectants is very consistent, occurring over a small temperature range. Fracture pattern, however, was affected by the cooling rate. The presence of tissue segments did not affect the onset temperature of fracture, but affected the fracture pattern. The continuum mechanics analysis solidified the hypothesis that fracture is driven by thermal stress, not by temperature per se, and allowed fracture strain to be inferred from observed fra…

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Research works printed but not linked: 21

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Metadata

Authors
Rabin, Y., Steif, P. S., Hess, K. C., Jimenez-Rios, J. L., & Palastro, M. C.
Venue
Cryobiology
Publisher
Elsevier BV
Published
2006-08
Volume
53
Issue
1
Pages
75-95
ISSN
0011-2240
DOI
10.1016/j.cryobiol.2006.03.013
Cited by
59
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

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