The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
ESCRTs and Fab1 Regulate Distinct Steps of Autophagy
Open accessAbstract
Eukaryotes use autophagy to turn over organelles, protein aggregates, and cytoplasmic constituents. The impairment of autophagy causes developmental defects, starvation sensitivity, the accumulation of protein aggregates, neuronal degradation, and cell death [1, 2]. Double-membraned autophagosomes sequester cytoplasm and fuse with endosomes or lysosomes in higher eukaryotes [3], but the importance of the endocytic pathway for autophagy and associated disease is not known. Here, we show that regulators of endosomal biogenesis and functions play a critical role in autophagy in Drosophila melanogaster. Genetic and ultrastructural analysis showed that subunits of endosomal sorting complex required for transport (ESCRT)-I, -II and -III, as well as their regulatory ATPase Vps4 and the endosomal PtdIns(3)P 5-kinase Fab1, all are required for autophagy. Although the loss of ESCRT or Vps4 function caused the accumulation of autophagosomes, probably because of inhibited fusion with the endolysosomal system, Fab1 activity was necessary for the maturation of autolysosomes. Importantly, reduced ESCRT functions aggravated polyglutamine-induced neurotoxicity in a model for Huntington's disease. Thus, this study links ESCRT function with autophagy and aggregate-induced neurodegeneration, thereby providing a plausible explanation for the fact that ESCRT mutations are involved in inherited neurodegenerative disease in humans [4].
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Cites
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes
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LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing.
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Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice
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Loss of autophagy in the central nervous system causes neurodegeneration in mice
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A Unified Nomenclature for Yeast Autophagy-Related Genes
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Histone deacetylase inhibitors arrest polyglutamine-dependent neurodegeneration in Drosophila
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“in the ommatidia of the compound eye and was performed essentially as described earlier [46], with the following modifications: Decapitated heads were glued to drops of 1% agarose on a microscope slide with transparent nail varnish.”
MethodRole and Regulation of Starvation-Induced Autophagy in the Drosophila Fat Body
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The FLP recombinase of yeast catalyzes site-specific recombination in the drosophila genome
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Cited by
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition).
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Self-citationRegulation mechanisms and signaling pathways of autophagy
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Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition) 1
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Self-citationRegulation of Mammalian Autophagy in Physiology and Pathophysiology
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Henne, W. M., Buchkovich, N. J., & Emr, S. D. (2011) · Developmental Cell · doi:10.1016/j.devcel.2011.05.015 · Cited by 1,496
Metadata
- Authors
- Rusten, T. E., Vaccari, T., Lindmo, K., Rodahl, L. M. W., Nezis, I. P., Sem-Jacobsen, C., Wendler, F., Vincent, J.-P., Brech, A., Bilder, D., & Stenmark, H.
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2007-10
- Volume
- 17
- Issue
- 20
- Pages
- 1817-1825
- ISSN
- 0960-9822 (ISSN-L)
- OpenAlex
- W2060579362
- Cited by
- 338
- References
- 46
- FWCI
- 12.98
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Rusten, T. E., Vaccari, T., Lindmo, K., Rodahl, L. M. W., Nezis, I. P., Sem-Jacobsen, C., Wendler, F., Vincent, J.-P., Brech, A., Bilder, D., & Stenmark, H. (2007). ESCRTs and Fab1 Regulate Distinct Steps of Autophagy. Current Biology. https://doi.org/10.1016/j.cub.2007.09.032
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
